wpisac

Comprehensive investigations of the Svisloch River phytoplankton were performed in 2006-2010. Samples were taken all-the-year-round at four sites: reservoirs Drozdy and Lake Komsomolskoye, sites along Vaneyev and Aranskaya streets (within the Minsk municipal section of the river). Species diversity of planktonic cyanobacteria and their contribution to phytoplankton abundance and biomass were determined. Potentially toxic cyanobacteria were detected based on the results of molecular-phylogenetic analysis. They contained a microcystin synthesis gene (mcyE). Obtained sequences belonged to the Microcystis and Anabaena genera, which were the most common in reservoirs worldwide and the most frequent agents of blooms. Several types of microcystins and a nontoxic peptide Oscillamide Y were detected in phytoplankton samples by means of LC/MSВ течение 2006-2010 гг. проводилось комплексное исследование фитопланктона р. Свислочь. Пробы отбирались круглогодично на четырех станциях в черте г. Минска: в зарегулированных водохранилищах Дрозды и Комсомольское озеро и на речных уча- стках в районе улиц Ванеева и Аранская. С помощью световой микроскопии определен видовой состав планктонных цианобактерий, оценен их вклад в численность и биомас- су фитопланктона. В результате молекулярно-филогенетических исследований выявле- ны потенциально токсичные цианобактерии, содержащие ген синтеза микроцистина (mcyE). Полученные последовательности принадлежат представителям родов Microcystis и Anabaena (= subg. Dolichospermum), которые широко распространены в водоемах мира и являются частыми возбудителями токсичных цветений. Несколько типов мик- роцистинов и нетоксичный пептид осцилламид Y были детектированы в пробах фито- планктона с помощью ВЭЖХ-МС

Investigation of the complex antibiotic INA-5812

A concentrate with the antimicrobial activity has been isolated from the culture broth of Streptomyces roseoflavus INA-Ac-5812. Its further fractionation by reversed-phase HPLC has resulted in six fractions. It has been established by MALDI-TOF and ESI-MSn precision mass-spectrometry methods that the main components of the complex antibiotic are several closely related compounds, presumably of a glycopeptide nature. The fraction containing an individual component with a mass of 1845.788 Da has been characterized by UV/Vis absorbance and fluorescence spectra, amino acid analysis, and derivatization with tris(2,6-dimethoxyphenyl)methyl cation. The activity of fractions against pathogenic microbes has been studied. The results allow the supposition that the INA-5812 antibiotic complex is a glyco- or lipoglycopeptide antibiotic of a new type, which is very promising for further study. © 2016, Pleiades Publishing, Ltd

Investigation of the complex antibiotic INA-5812

A concentrate with the antimicrobial activity has been isolated from the culture broth of Streptomyces roseoflavus INA-Ac-5812. Its further fractionation by reversed-phase HPLC has resulted in six fractions. It has been established by MALDI-TOF and ESI-MSn precision mass-spectrometry methods that the main components of the complex antibiotic are several closely related compounds, presumably of a glycopeptide nature. The fraction containing an individual component with a mass of 1845.788 Da has been characterized by UV/Vis absorbance and fluorescence spectra, amino acid analysis, and derivatization with tris(2,6-dimethoxyphenyl)methyl cation. The activity of fractions against pathogenic microbes has been studied. The results allow the supposition that the INA-5812 antibiotic complex is a glyco- or lipoglycopeptide antibiotic of a new type, which is very promising for further study. © 2016, Pleiades Publishing, Ltd