A pandemic strain of calicivirus threatens rabbit industries in the Americas-4

<p><b>Copyright information:</b></p><p>Taken from "A pandemic strain of calicivirus threatens rabbit industries in the Americas"</p><p>http://www.virologyj.com/content/4/1/96</p><p>Virology Journal 2007;4():96-96.</p><p>Published online 2 Oct 2007</p><p>PMCID:PMC2147015.</p><p></p>-capture ELISA using type-specific HRP-conjugated monoclonal antibodies (MAb). MAb 1H8 is specific for the original RHDV serotype, MAb 3B12 is specific for the new RHDVa pandemic strain, and MAb 2B4 recognizes a shared epitope. The four U.S. RHDV isolates, Mexico 1989 isolate, an Italian isolate, and Korean isolate were compared in comparison with a control liver homogenate derived from an uninfected rabbit (Normal Liver). All U.S. isolates were recognized by MAb 3B12 as belonging to the RHDVa pandemic strain

A pandemic strain of calicivirus threatens rabbit industries in the Americas-0

<p><b>Copyright information:</b></p><p>Taken from "A pandemic strain of calicivirus threatens rabbit industries in the Americas"</p><p>http://www.virologyj.com/content/4/1/96</p><p>Virology Journal 2007;4():96-96.</p><p>Published online 2 Oct 2007</p><p>PMCID:PMC2147015.</p><p></p>ion) is shown (H&E stain, 40× objective). Note the acute hepatocellular necrosis characterized by destruction and disassociation of hepatocytes, loss of cellular organization, and evidence of acidophilic bodies (white arrow head), karyorrhexis (white arrow), and necrotic or apoptotic hepatocytes (black arrow head). . A liver section from the surviving infected rabbit (day 21 post infection) exhibited normal liver morphology (H&E staine, 40× objective). . Transmission electron micrograph showing the ultrastructure of a hepatocyte from a fatally infected rabbit revealed the presence of 26.5 nm +/- 1.9 diameter viral particles with morphology characteristic of caliciviruses. . An example of ultrastructural changes to a hepatocyte from one of the fatally infected rabbits. Note the margination of chromatin (Ch) in the nucleus (Nu), and disruption of cristae in mitochondria (Mt). Often, an abnormal condensation of the endoplasmic reticulum (ER) was observed. The inset shows an abnormally dense reticular network

A pandemic strain of calicivirus threatens rabbit industries in the Americas-3

<p><b>Copyright information:</b></p><p>Taken from "A pandemic strain of calicivirus threatens rabbit industries in the Americas"</p><p>http://www.virologyj.com/content/4/1/96</p><p>Virology Journal 2007;4():96-96.</p><p>Published online 2 Oct 2007</p><p>PMCID:PMC2147015.</p><p></p>RHDV and 1 isolate of a non-pathogenic rabbit calicivirus (RCV) is shown. The top reference sequence for the alignment came from the Brescia 1989 strain (BS89 Italy) and identical amino acids were indicated by a dot. Note the large number of shared amino acid substitutions within the RHDVa clade (shaded blue)

A pandemic strain of calicivirus threatens rabbit industries in the Americas-1

<p><b>Copyright information:</b></p><p>Taken from "A pandemic strain of calicivirus threatens rabbit industries in the Americas"</p><p>http://www.virologyj.com/content/4/1/96</p><p>Virology Journal 2007;4():96-96.</p><p>Published online 2 Oct 2007</p><p>PMCID:PMC2147015.</p><p></p>L W. One thousand bootstrap replicates were subjected to protein distance and UPGMA methods and the consensus phylogenetic tree is shown. The VP60 region of a non-pathogenic rabbit calicivirus (RCV) was used as an outgroup. Two clades, one representing the original RHDV serotype and a second representing the new RHDVa subtype were identified. Bootstrap values greater than 50% are displayed above the tree branches

A pandemic strain of calicivirus threatens rabbit industries in the Americas-2

<p><b>Copyright information:</b></p><p>Taken from "A pandemic strain of calicivirus threatens rabbit industries in the Americas"</p><p>http://www.virologyj.com/content/4/1/96</p><p>Virology Journal 2007;4():96-96.</p><p>Published online 2 Oct 2007</p><p>PMCID:PMC2147015.</p><p></p> monoclonal antibodies. Previous studies and communication from Lorenzo Capucci [35] have determined that monoclonal antibodies 1H8, 2A10, and 1H3 recognize the original serotype of RHDV while antibodies 3D4, 3B12, 2E1, 3D6, and 5D11 recognize RHDVa-specific epitopes. Additional monoclonal antibodies used (6H6, 1F10, 3H6, 6F9, 2B4, and 2G3) were not subtype-specific. The IA-00 isolate (black bars) correlated in antibody recognition profile to a prototype RHDVa strain, Pavia 1997 (grey bars). The Brescia 1989 strain (stippled bars) was used as an original RHDV serotype virus control. Normal liver from an uninfected rabbit served as a negative control (white bars)