Table S1 from A sterilizable, biocompatible, tropoelastin surface coating immobilized by energetic ion activation

The fitting parameters for the curves shown in Fig. 1B

Mechanism of EPC attachment to rhTE.

<p>(A) EPCs attached to 40 μg/ml rhTE in the presence of α-lactose, β-lactose, heparan sulfate, or EDTA. (B) Attachment of EPCs to 40 μg/ml rhTE in the presence of Ca²⁺, Mg²⁺, or Mn²⁺. (C) and (D) Inhibition of EPC attachment and spreading on 40 μg/ml rhTE using antibodies to integrins α₂β₁, α₅β₁, and α_vβ₃. Error bars represent S.E.M. of triplicate measurements.</p

Mechanism of EPC attachment to truncated tropoelastin constructs.

<p>(A) and (B) EPCs attached to 40 μg/ml N25 and N18 respectively, in the presence of α-lactose, β-lactose, heparan sulfate or EDTA. (C) and (D) Attachment of EPCs to 40 μg/ml N25 and N18, respectively, in the presence of Ca²⁺, Mg²⁺, or Mn²⁺. (E) and (F), Inhibition of EPC spreading on 40 μg/ml N25 and N18 respectively, using antibody that inhibits binding to integrin α_vβ₃. Error bars represent S.E.M. of triplicate measurements.</p

Schematic diagram showing full-length rhTE and constructs N18, N25 and N10.

<p>Key domain features are indicated.</p

OEC characterization by flow cytometry.

<p>A) Stained cells are shown as blue histograms, while unstained controls are shown in black. The percentage of positive cells is shown in the top right of each graph. The OECs are CD34/31/54/VEGFR2 positive and CD45/14 negative. B) Representative images of the binding of isothiocyanate-Ulex europaeus agglutinin I lectin binding (ULEX), uptake of acetylated low density lipoprotein (AcLDL) and staining for CD31 by EPCs (bottom row of panel) but not by fibroblasts (top row of panel). Together, these results are indicative of a positive endothelial cell phenotype.</p