2 research outputs found
Low temperature co-fired ceramic (LTCC)based biosensor for detection of vanadium using immobilized Arachis hypogaea alkaline phosphatase on multi walled carbon nanotubes ethyl cellulose sponge matrix
916-920Studies on enzyme based thermometric biosensor are limited.
Here, we report on fabrication of an alkaline phosphate based
thermometric biosensor. We designed alkaline phosphatase
inhibition based biosensor for detection of vanadium using
immobilized alkaline phosphatase on multi walled carbon nano
tubes (MWCTs) ethyl cellulose sponge matrix. We isolated
protein from plant source, partially purified and fabricated a
miniature ceramic viz. LTCC (low temperature co-fired ceramics
technology) based biosensor for detection of vanadium. This
biosensor consists of a microreaction chamber with buried
heaters. Alkaline phosphatase has been isolated from the seeds of
‘Arachis hypoghaea’ was studied for its biochemical properties
viz. optimum pH and temperature. The partially purified enzyme
was immobilized using carboxyl-functionalised carbon nanotubes
(CNTs) by cross linking with epichlorohydrin (ECH) along with a
matrix of ethyl cellulose. The developed LTCC based biosensor
on testing indicated its linear response to vanadium concentration
up to 9 mM with a relatively high sensitivity of about
147 nA/mM. Thus, we have demonstrated a LTCC based
biosensor using immobilized alkaline phosphatase for detection of
vanadium
Low temperature co-fired ceramic (LTCC)- based biosensor for detection of vanadium using immobilized Arachis hypogaea alkaline phosphatase on multi walled carbon nanotubes ethyl cellulose sponge matrix
Studies on enzyme based thermometric biosensor are limited. Here, we report on fabrication of an alkaline phosphate based thermometric biosensor. We designed alkaline phosphatase inhibition based biosensor for detection of vanadium using immobilized alkaline phosphatase on multi walled carbon nano tubes (MWCTs) ethyl cellulose sponge matrix. We isolated protein from plant source, partially purified and fabricated a miniature ceramic viz. LTCC (low temperature co-fired ceramics technology) based biosensor for detection of vanadium. This biosensor consists of a microreaction chamber with buried heaters. Alkaline phosphatase has been isolated from the seeds of ‘Arachis hypoghaea’ was studied for its biochemical properties viz. optimum pH and temperature. The partially purified enzyme was immobilized using carboxyl-functionalised carbon nanotubes (CNTs) by cross linking with epichlorohydrin (ECH) along with a matrix of ethyl cellulose. The developed LTCC based biosensor on testing indicated its linear response to vanadium concentration up to 9 mM with a relatively high sensitivity of about 147 nA/mM. Thus, we have demonstrated a LTCC based biosensor using immobilized alkaline phosphatase for detection of vanadium