Neutrophil adhesion onto untreated (Control) and TNFα-treated WT and HO-2^−/− aortic endothelial cells (mAEC).

<p>Neutrophil adherence is measured as the fluorescence intensity associated with mAEC and is given in relative fluorescence units (RFU). Results are the mean ± SE; n = 3; **p<0.005 from WT mAEC; †p<0.05 from untreated HO-2^−/− mAEC.</p

Real-time PCR analysis of HO-1 mRNA expression in neutrophils purified from peripheral blood of control and neutrophil (N)-depleted WT and HO-2^−/− mice 2 days after injury.

<p>Results are the mean ± SE; n = 3; *p<0.01 from neutrophils purified from control WT mice; **p<0.05 from neutrophils purified from neutrophil-depleted HO-2^−/− mice.</p

Infiltration of Inflammatory cells in the cornea at day 2 after epithelial injury.

<p>(<b>A</b>) Representative H&E staining of corneas from uninjured and injured control and neutrophil-depleted WT and HO-2^−/− mice 2 days after injury. (<b>B</b>) Number of neutrophils in peripheral blood of control and neutrophil (N)-depleted WT and HO-2−/− mice 2 days after injury. Results are mean ± SE; n = 3; *p<0.0001 from control WT; **p<0.0001 from control HO-2^−/− mice; † p<0.05 from control WT.</p

Effect of neutrophil depletion on wound healing.

<p>(<b>A</b>) Representative images of fluorescein-stained corneas at day 0 and day 2 after injury in control and neutrophil (N)-depleted WT and HO-2^−/− mice. (<b>B</b>) Wound closure as percent change from day 0. Results are mean ± SE; n = 5–7; *p<0.005 from control WT mice; **p<0.005 from control HO-2^−/− mice; † p<0.01 from control WT mice; ‡ p<0.005 from neutrophil-depleted WT mice.</p

Immunofluoescence analysis of (A) Gr-1- and (B) CD68-positive cells in corneas from control and neutrophil-depleted WT and HO-2^−/− mice 2 days after injury.

<p>Images were taken at 63× magnification and are representative of three separate analyses.</p

Real-time PCR analysis of mRNA expression of (A) midkine (Mdk) and (B) VE-Cadherin in untreated aortic endothelial cells (mAEC) from WT and HO-2^−/−.

<p>Results are the mean ± SE; n = 4 to 5; *p<0.005 from WT mAEC.</p

Saliva levels of S100B in neonates with normal, BG/W score 1–2 and BG/W score 3–4.

<p>The box plots represent the medians and interquartile ranges for each group. ^*^*P<0.001 global BG/W vs severe BG/W injury; ^*P<0.001 vs normal MRI group.</p

Mean saliva S100B concentrations (µg/mL) expressed as MoM [lower and upper 95% Confidence Interval (CI)] at birth (T0) and at 4 (T1), 8 (T2), 12 (T3), 16 (T4), 20 (T5), 24 (T6), 48 (T7), 72 (T8) and 96 (T9) hours after birth in Reference Group (n = 244) and in asphyxiated full-term newborns with good (Group A) or severe (Group B) neurological outcome at 12-months follow-up.

<p>^*P<0.001 vs Healthy Group and asphyxiated full-term newborns with good neurological prognosis (Group A).^*P<0.001 vs Group B values at 48, 72 and, 96 hours</p><p>Salivary S100B concentrations were significantly higher in neonates belonging to Group B at all monitoring time-points (p<0.001, for all).</p><p>Statistical evaluation of differences among Groups at each time point was performed by using the Kruskal-Wallis test followed by the Dunn’s post test</p><p>Mean saliva S100B concentrations (µg/mL) expressed as MoM [lower and upper 95% Confidence Interval (CI)] at birth (T0) and at 4 (T1), 8 (T2), 12 (T3), 16 (T4), 20 (T5), 24 (T6), 48 (T7), 72 (T8) and 96 (T9) hours after birth in Reference Group (n = 244) and in asphyxiated full-term newborns with good (Group A) or severe (Group B) neurological outcome at 12-months follow-up.</p

Perinatal clinical characteristics and outcomes in asphyxiated newborns with normal (Group A) or abnormal (Group B) neurological outcome and in healthy subjects (Reference Group, R Group).

<p>Values are expressed as mean ± SD. n.p.: not performed.</p><p>^*P<0.01 vs controls.</p><p>Perinatal clinical characteristics and outcomes in asphyxiated newborns with normal (Group A) or abnormal (Group B) neurological outcome and in healthy subjects (Reference Group, R Group).</p

Laboratory parameters recorded at birth in asphyxiated newborns with normal (Group A) or abnormal (Group B) neurological outcome and in healthy subjects (Reference Group, R Group).

<p>Values are expressed as mean ± SD.</p><p>^*P<0.0001 vs controls.</p><p>Laboratory parameters recorded at birth in asphyxiated newborns with normal (Group A) or abnormal (Group B) neurological outcome and in healthy subjects (Reference Group, R Group).</p