Diurnal expression pattern of Cryptochrome (A) and Phytochrome (B) transcripts analyzed by QRT-PCR in <i>wt</i>, <i>cry1a</i>- and <i>CRY2OX</i> GA₃-treated tomato plants.

<p>Results are presented as a ratio after normalization with β-actin. Yellow and dark bars along the horizontal axis represent light and dark periods, respectively. Time points are measured in hours from dawn (zeitgeber Time [ZT]); data at ZT24 constitute a replotting of those at ZT0. The control data, of gene expression in the absence of hormone applications, are reproduced, for clarity, from those in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030121#pone.0030121.s001" target="_blank">Figure S1</a>. Data shown are the average of two biological replicates, with error bars representing SEM. Hormone-treated plant transcripts significantly different from the corresponding ones of control plants are marked with a * (Student's t test, P≤0.05), two ** (Student's t test, P≤0.01) and three *** (Student's t test, P≤0.001).</p

Diurnal expression pattern of <i>CAB4</i> (A) and <i>GIGANTEA</i> (B) transcripts analyzed by QRT-PCR in <i>wt</i>, <i>cry1a</i>- and <i>CRY2OX</i> hormone-treated tomato plants.

<p>Results are presented as a ratio after normalization with β-actin. Yellow and dark bars along the horizontal axis represent light and dark periods, respectively. Time points are measured in hours from dawn (zeitgeber Time [ZT]); data at ZT24 constitute a replotting of those at ZT0. The control data, of gene expression in the absence of hormone applications, are reproduced, for clarity, from those in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030121#pone.0030121.s001" target="_blank">Figure S1</a>. Data shown are the average of two biological replicates, with error bars representing SEM. Hormone-treated plant transcripts significantly different from the corresponding ones of control plants are marked with a * (Student's t test, P≤0.05), two ** (Student's t test, P≤0.01) and three *** (Student's t test, P≤0.001).</p

Diurnal expression pattern of Cryptochrome (A) and Phytochrome (B) transcripts analyzed by QRT-PCR in <i>wt</i>, <i>cry1a</i>- and <i>CRY2OX</i> IAA-treated tomato plants.

<p>Results are presented as a ratio after normalization with β-actin. Yellow and dark bars along the horizontal axis represent light and dark periods, respectively. Time points are measured in hours from dawn (zeitgeber Time [ZT]); data at ZT24 constitute a replotting of those at ZT0. The control data, of gene expression in the absence of hormone applications, are reproduced, for clarity, from those in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030121#pone.0030121.s001" target="_blank">Figure S1</a>. Data shown are the average of two biological replicates, with error bars representing SEM. Hormone-treated plant transcripts significantly different from the corresponding ones of control plants are marked with a * (Student's t test, P≤0.05), two ** (Student's t test, P≤0.01) and three *** (Student's t test, P≤0.001). Data from control plants are replotted from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030121#pone-0030121-g002" target="_blank">Figure 2</a>.</p

Number of transcription patterns altered in at least three points per cycle, by ZEA, GIB, AUX and ABA phyto-hormones in <i>wt</i>, <i>cry1a-</i> and <i>CRY2OX</i> genotypes.

<p>We considered four cryptochrome (CRYs (4)) and five phytochrome (PHYs (5)) gene transcripts. In the squares is indicated the number of altered patterns for each hormone.</p

Diurnal expression pattern of Cryptochrome (A) and Phytochrome (B) transcripts analyzed by QRT-PCR in <i>wt</i>, <i>cry1a</i>- and <i>CRY2OX</i> ABA-treated tomato plants.

<p>Results are presented as a ratio after normalization with β-actin. Yellow and dark bars along the horizontal axis represent light and dark periods, respectively. Time points are measured in hours from dawn (zeitgeber Time [ZT]); data at ZT24 constitute a replotting of those at ZT0. The control data, of gene expression in the absence of hormone applications, are reproduced, for clarity, from those in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030121#pone.0030121.s001" target="_blank">Figure S1</a>. Data shown are the average of two biological replicates, with error bars representing SEM. Hormone-treated plant transcripts significantly different from the corresponding ones of control plants are marked with a * (Student's t test, P≤0.05), two ** (Student's t test, P≤0.01) and three *** (Student's t test, P≤0.001). Data from control plants are replotted from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030121#pone-0030121-g002" target="_blank">Figure 2</a>.</p

Concentrations of provitamin A carotenoids, lutein, phytoene, phytofluene and α-tocopherol in fetal bovine serum and Caco-2 intestinal cells before and after 4h incubation with diluted mixed micelles generated during <i>in vitro</i> digestion of GP17 and GP30 boiled tubers.

<p>Concentrations of provitamin A carotenoids, lutein, phytoene, phytofluene and α-tocopherol in fetal bovine serum and Caco-2 intestinal cells before and after 4h incubation with diluted mixed micelles generated during <i>in vitro</i> digestion of GP17 and GP30 boiled tubers.</p

Carotenoid and α-tocopherol contents in raw and boiled wild-type and golden potato (GP) tubers.

<p>Carotenoid and α-tocopherol contents in raw and boiled wild-type and golden potato (GP) tubers.</p

Contribution of a 150 g serving of boiled wild type and golden potatoes to the estimated average requirement (EAR)^* for retinol activity equivalents (RAE) and vitamin E [44].

<p>Contribution of a 150 g serving of boiled wild type and golden potatoes to the estimated average requirement (EAR)^{<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0187102#t004fn001" target="_blank">*</a>} for retinol activity equivalents (RAE) and vitamin E [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0187102#pone.0187102.ref044" target="_blank">44</a>].</p

Relative accumulation (%) of provitamin A carotenes, lutein, carotenoid precursors and αTC by Caco-2 cells.

<p>Data are mean ± SD; n = 6 monolayers of Caco-2 cells incubated with the mixed micelle fraction generated by simulated digestion of a pooled sample prepared from 10 tubers from 5 plants of each genotypes. Different letters above bars indicate that means differ significantly (<i>p <</i> 0.01).</p

Bioaccessible provitamin A carotenoids, lutein, phytoene, phytofluene and α-tocopherol in digested potato tubers (μg/g DW).

<p>Bioaccessible provitamin A carotenoids, lutein, phytoene, phytofluene and α-tocopherol in digested potato tubers (μg/g DW).</p