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    Sequence Analysis of 96 Genomic Regions Identifies Distinct Evolutionary Lineages within CC156, the Largest <i>Streptococcus pneumoniae</i> Clonal Complex in the MLST Database

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    <div><p>Multi-Locus Sequence Typing (MLST) of <i>Streptococcus pneumoniae</i> is based on the sequence of seven housekeeping gene fragments. The analysis of MLST allelic profiles by eBURST allows the grouping of genetically related strains into Clonal Complexes (CCs) including those genotypes with a common descent from a predicted ancestor. However, the increasing use of MLST to characterize <i>S. pneumoniae</i> strains has led to the identification of a large number of new Sequence Types (STs) causing the merger of formerly distinct lineages into larger CCs. An example of this is the CC156, displaying a high level of complexity and including strains with allelic profiles differing in all seven of the MLST loci, capsular type and the presence of the Pilus Islet-1 (PI-1). Detailed analysis of the CC156 indicates that the identification of new STs, such as ST4945, induced the merging of formerly distinct clonal complexes. In order to discriminate the strain diversity within CC156, a recently developed typing schema, 96-MLST, was used to analyse 66 strains representative of 41 different STs. Analysis of allelic profiles by hierarchical clustering and a minimum spanning tree identified ten genetically distinct evolutionary lineages. Similar results were obtained by phylogenetic analysis on the concatenated sequences with different methods. The identified lineages are homogenous in capsular type and PI-1 presence. ST4945 strains were unequivocally assigned to one of the lineages. In conclusion, the identification of new STs through an exhaustive analysis of pneumococcal strains from various laboratories has highlighted that potentially unrelated subgroups can be grouped into a single CC by eBURST. The analysis of additional loci, such as those included in the 96-MLST schema, will be necessary to accurately discriminate the clonal evolution of the pneumococcal population.</p> </div

    CC156 strain panel used in this study.

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    <p>For each strain name, ST, serotype/serogroup, country of isolation, number of MLST alleles in common with ST156 and ST4945, data source, strain source and lineage (as identified by 96-MLST hierarchical clustering, <i>see</i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061003#pone-0061003-g002" target="_blank">Figure 2</a>) are indicated.</p

    Minimum Spanning Tree analysis based on 96-MLST allelic profiles identifies seven distinct lineages by imposing a maximum threshold of 75 different loci.

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    <p>The Minimum Spanning Tree analysis was performed by using PHYLOVIZ on the 96-MLST alleles of the 66 strains considered in this study. The lineages identified by applying the threshold of 75/96 different loci are highlighted with shadowed shapes and named according to the lineage identification of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061003#pone-0061003-g002" target="_blank">Figure 2</a>.</p
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