14 research outputs found
O CUIDADO EM SAÚDE E AS IMPLICAÇÕES PARA OS CUIDADORES DOMICILIARES
RESUMO: O artigo trata da temática do cuidado domiciliar em saúde, modalidade de atendimento que tem crescido em vários países, impulsionada pelas transições demográficas e epidemiológicas dos últimos anos. Com foco em duas experiências, o trabalho apresenta os resultados de uma pesquisa com o objetivo de analisar o perfil dos cuidadores de dois programas de atenção em saúde domiciliar sendo, um público e outro privado desenvolvido em cidades localizadas na Região da Grande Florianópolis. Trata-se de uma pesquisa qualitativa com aplicação de entrevistas com cuidadores de pessoas em internação domiciliar. Como principais resultados aponta-se que a família tem sido cada vez mais responsabilizada pelo cuidado em saúde, principalmente as mulheres alterando a dinâmica familiar que implica nas condições objetivas de vida
Induction of apoptosis by SR in normoxic and hypoxic conditions on REH cells.
<p>Cells were treated with 0 to 30 µM SR for 24 h. The % of apoptotic cells recorded in the untreated cultures was subtracted from that observed in cultures treated with SR. Data are presented as mean ± SEM of at least four different experiments.</p
Effect of SR on p53, Bax and Bcl-2 mRNA relative expression calculated through the 2<sup>-ΔΔC</sup>t method and determined in normoxic and hypoxic conditions on REH cells.
<p>Cells were treated with 0 to 30 µM SR for 6, 24 or 48 h. Data are presented as mean ± SEM of at least four different experiments.</p
Cell-cycle distribution following 24 h culture in the absence or presence of SR in normoxic (A) and hypoxic (B) conditions on REH cells.
<p>Data are means ± SEM of four independent experiments.</p
Effect of SR on ROS levels determined in normoxic (A) and hypoxic (B) conditions on REH cells.
<p>Cells were treated for 30–360 min with SR to analyze the oxidation state of the cell by using DCFH-DA as fluorogenic probe. Results are expressed as DCF (2′,7′-dichlorodihydrofluorescein) fluorescence (% of control) and are means ± SEM of four independent experiments.</p
SR induces DNA damage in REH cells.
<p>Cells were treated with increasing concentrations of, or for increasing time intervals with, SR and immediately assayed for DNA breakage with FHA. A) DNA single strand breakage induced by 3 h SR treatment in REH cells. B) DNA single strand breakage induced by 30 µM SR in REH cells as a function of incubation time. Data are presented as mean ± SEM of three different experiments. C) and D) Representative, digitally pseudocolored (ICA look up table of the Image J software) micrographs of FHA-processed control (C) or 30 µM SR-treated (D) REH cells are also shown: note the wide halos in D as compared to C. Also shown (panel A, inset) the extent of DNA double strand breakage caused by SR (30 µM for 3 h) or etoposide (1 µg/mL for 3 h) in REH cells. Data are expressed as NSF (nuclear diffusion factor), which represents the ratio between the total area of the halo and nucleus and that of the nucleus.</p
Clinical features of patients.
a<p>a combination drug protocol used as induction chemotherapy and consisting of three days of anthracyclines and seven days of cytarabine;</p>b<p>allogeneic HSCT: transplantation of allogeneic hematopoietic stem cells.</p
Clinical features of patients.
a<p>WBC: number of cells/mm<sup>3</sup> whole blood;</p>b<p>internal tandem duplication.</p
Effect of different inhibitors on <i>Hemidesmus</i>-induced [Ca<sup>2+</sup>]<sub>i</sub> raise.
<p>Effects of nifedipine (A), econazole (B), aristolochic acid (C) and thapsigargin (D) on <i>Hemidesmus</i>-induced [Ca<sup>2+</sup>]<sub>i</sub> raise. Data are means ± SEM of three independent experiments.</p
Effects of Hemidesmus on Bax-to-Bcl-2 ratio and cell proliferation.
<p>Bax-to-Bcl-2 ratio (A), cell proliferation (B), cell-cycle distribution (C–E), and cyclin A2, cyclin E, CDK2, and p21 protein levels (F) following 24 h culture in the absence or presence of <i>Hemidesmus</i>. Data are means ± SEM of four independent experiments.</p