Chronic adolescent stress increases exploratory behavior but does not appear to change the acute stress response in adult male C57BL/6 mice

Chronic stress exposure in adolescence can lead to a lasting change in stress responsiveness later in life and is associated with increased mental health issues in adulthood. Here we investigate whether the Chronic Social Instability (CSI) paradigm influences the behavioral and molecular responses to novel acute stressors in mice, and whether it alters physiological responses influenced by the noradrenergic system. Using large cohorts of mice, we show that CSI mice display a persistent increase in exploratory behaviors in the open field test alongside small but widespread transcriptional changes in the ventral hippocampus. However, both the transcriptomic and behavioral responses to novel acute stressors are indistinguishable between groups. In addition, the pupillometric response to a tail shock, known to be mediated by the noradrenergic system, remains unaltered in CSI mice. Ultra-high performance liquid chromatography analysis of monoaminergic neurotransmitter levels in the ventral hippocampus also shows no differences between control or CSI mice at baseline or in response to acute stress. We conclude that CSI exposure during adolescence leads to persistent changes in exploratory behavior and gene expression in the hippocampus, but it does not alter the response to acute stress in adulthood and is unlikely to alter the function of the noradrenergic system

3He in Planetary Nebulae: A Challenge to Stellar Evolution Models

The discrepancy between the observed abundances of 3He in the ISM and those predicted by stellar and galactic chemical evolution remains largely unexplained. In this paper, we attempt to shed some light on this unsolved problem by presenting a quantitative comparison of the 3He abundances recently measured in six planetary nebulae (PNe) with the corresponding predictions of stellar evolution theory. The determination of the mass of the PNe progenitors allows us to dismiss, to a good degree of confidence, the hypothesis that the abundance of 3He in the envelope of all low-mass stars is strongly reduced with respect to the standard theoretical values by some mixing mechanism acting in the latest phases of stellar evolution. The abundance versus mass correlation, allowance made for the limitation of the sample, is in fact found to be fully consistent with the classical prediction of stellar evolution. We examine the implications of this result on the galactic evolution of 3He with the help of a series of models with standard and non-standard nucleosynthesis prescriptions. The results are found to be consistent with the observed galactic abundances only if the vast majority of low-mass stars follows non-standard prescriptions. This implies that either the sample of PNe nebulae under exam is highly biased, or the solution to the 3He problem lies elsewhere.Comment: 13 pages, LaTeX, 4 Figures. To be published in The Astrophysical Journa

Chronic adolescent stress increases exploratory behavior but does not appear to change the acute stress response in adult male C57BL/6 mice

Chronic stress exposure in adolescence can lead to a lasting change in stress responsiveness later in life and is associated with increased mental health issues in adulthood. Here we investigate whether the Chronic Social Instability (CSI) paradigm influences the behavioral and molecular responses to novel acute stressors in mice, and whether it alters physiological responses influenced by the noradrenergic system. Using large cohorts of mice, we show that CSI mice display a persistent increase in exploratory behaviors in the open field test alongside small but widespread transcriptional changes in the ventral hippocampus. However, both the transcriptomic and behavioral responses to novel acute stressors are indistinguishable between groups. In addition, the pupillo-metric response to a tail shock, known to be mediated by the noradrenergic system, remains unaltered in CSI mice. Ultra-high performance liquid chromatography analysis of monoaminergic neurotransmitter levels in the ventral hippocampus also shows no differences between control or CSI mice at baseline or in response to acute stress. We conclude that CSI exposure during adolescence leads to persistent changes in exploratory behavior and gene expression in the hippocampus, but it does not alter the response to acute stress in adulthood and is unlikely to alter the function of the noradrenergic system.ISSN:2352-289