7 research outputs found
Additional file 3: Figure S3. of TP53 gene mutation analysis in chronic lymphocytic leukemia by nanopore MinION sequencing
Sanger analysis on CLL#12 showing the mutation g.7578406CâÂÂ>âÂÂT. It is not detectable by the analysis software due to the low intensity of variant peak. The presence of the peaks for T base both in forward and in reverse sequences confirm the mutation. (TIF 3259 kb
Additional file 2: of TP53 gene mutation analysis in chronic lymphocytic leukemia by nanopore MinION sequencing
Reformatted file of âÂÂgene variation IARC TP53 databaseâ to use in Galaxy workflow for variants annotation. (TXT 1603 kb
Cytokine production profile and lytic activities of <i>Aspergillus-</i>specific T cells.
<p><b>A, B.</b> The frequencies of <i>Aspergillus</i> specific T cells producing IFNÎł, IL-10, IL-4 or IL-17A against the seven recombinant antigens, either as EM (dark gray portion of the columns) or CM (light gray portion of the columns), are shown as mean % positive cells Âą standard deviation, computed over the 22 patients with IA. Results are expressed as percentages of either CD4<sup>+</sup> T cells (A) or of CD8<sup>+</sup> T cells (B). <b>C.</b> White columns represent rates of hyphal damage by <i>Aspergillus</i>-specific T cells expanded from PBMCs stimulated with heat killed <i>Aspergillus</i> conidia. Black columns represent rates of hyphal damage by <i>Aspergillus</i>-specific T cells expanded from PBMCs stimulated with <i>Aspergillus</i> recombinant antigens (GEL1, PEP1, Îą1â3 glucan, β1â3 glucan) at two and twenty two-hour cultures. E/Tâ=âeffector/target cell ratio. <b>D.</b> Rates of hyphal damage by <i>Aspergillus</i>-specific T cells expanded with <i>Aspergillus</i> either heat killed conidia (white columns) or recombinant antigens (GEL1, PEP1, Îą1â3 glucan, β1â3 glucan) (black columns); by the supernatant of cultures of <i>Aspergillus</i>-specific T cell lines only; by antigen presenting cells (APCs); by polymophonuclears (PMNs) and by combinations of the different three cell fractions from three further patients with IA, at two-hour cultures. *â=â<i>P</i><.05. E/Tâ=âeffector/target cell ratio. Results are expressed as mean+âStandard Deviation.</p
Clinical characteristics of the patients.
<p>AMLâ=âacute myeloid leukemia; ALLâ=âacute lymphoblastic leukemia; CLLâ=âchronic lymphocytic leukemia; AlloSCTâ=âallogeneic stem cell transplant; AutoSCTâ=âautologous stem cell transplant; DLCBLâ=âdiffuse large B cell lymphoma; NHLâ=ânon Hodgkin lymphoma; MDSâ=âmyelodysplastic syndrome; CNSâ=âcentral nervous system; IAâ=âproven Invasive Aspergillosis; RSVâ=ârespiratory syncytial virus; CMVâ=âcytomegalovirus; E. coliâ=âEscherichia coli; posâ=âpositive; negâ=ânegative; L-AmBâ=âliposomal amphotericin B.</p
Kinetics of specific T-cell responses to the seven recombinant antigens of <i>Aspergillus</i> by IFN-Îł, IL-10, and IL-4 ELISpot assay in 22 patients with invasive aspergillosis (IA), patient 1 to patient 10.
<p>Kinetics of specific T-cell responses to the seven recombinant antigens of <i>Aspergillus</i> by IFN-Îł, IL-10, and IL-4 ELISpot assay in 22 patients with invasive aspergillosis (IA), patient 1 to patient 10.</p
Kinetics of specific T-cell responses to the seven recombinant antigens of <i>Aspergillus</i> by IFN-Îł, IL-10, and IL-4 ELISpot assay in 22 patients with invasive aspergillosis (IA), patient 11 to patient 22.
<p>Blue columns represent the number of <i>Aspergillus</i>-specific T cells producing IFN-Îł; yellow columns represent the number of <i>Aspergillus</i>-specific T cells producing IL-10; green columns represent the number of <i>Aspergillus</i>-specific T cells producing IL-17A; red columns represent the number of <i>Aspergillus</i>-specific T cells producing IL-4; dark gray background represents T-cell responses in wells with anti-CD3 antibody. Vertical axis shows the number of spot-forming cells (SFCs) per million peripheral blood mononuclear cells (PBMCs); horizontal axis indicates the antigen, which the specific immune responses are directed to and the phase of IA calculated as the number of days elapsed from the radiological diagnosis of the infection (t1, from the radiologic disclosure of the infectious lesions until day +14; t2, from day +15 until day +30; t3, from day +31 to day +45; t4, from day +46 to day 60 days; t4+ >61 days). CRF1â=â ortholog of Crh1p associated in β1,6 glucan-chitin linkages; PEP1â=â aspartic protease; GEL1â=â1,3-β glucanosyltransferase; Îą1â3 gluâ=âÎą1â3 glucan; β1â3 gluâ=âβ1â3 glucan; SOD1â=â superoxide dismutase; and GMâ=âgalactomannan. Ptâ=âpatient. *â=âpatients with unfavorable outcome. <sup>â§</sup>â=âpatients with probable IA. The red dotted line divides the two groups of patients: from pt n° 1 to pt n° 15 with <i>Aspergillus</i>-specific T cells producing IFN-Îł to two or more antigens; from pt n° 16 to pt n° 22 without <i>Aspergillus</i>-specific T cells producing IFN-Îł or with such cells to only one antigen.</p
<i>Aspergillus</i>-specific T-cell responses to the 7 recombinant antigens during the course of Invasive Aspegillosis (IA).
<p><b>A,B,C,D. A.</b> Box plots showing specific immune responses producing IL-10 (yellow columns) to any single recombinant antigen. The horizontal axis represents the antigen, which the specific immune responses are directed to. <b>B.</b> Box plot showing specific immune responses producing IL-10 (yellow columns) against all the 7 recombinant antigens at the four phases of the IA. The horizontal axis represents the different phases of IA. <b>C.</b> Box plot showing specific immune responses producing IFN-Îł (blue columns) to any single recombinant antigen. The horizontal axis represents the antigen, which the specific immune responses are directed to. <b>D.</b> Box plot showing specific immune responses producing IFN-Îł (blue columns) against all the 7 recombinant antigens at the four phases of the IA. The horizontal axis represents the different phases of IA. The vertical axis shows the number of spot-forming cells (SFCs) per million peripheral blood mononuclear cells (PBMCs).The upper horizontal line represents the upper adjacent value. The upper hinge of the boxes represents the 75<sup>th</sup> percentile. The middle horizontal line of the boxes represents the median value. The lower hinge of the boxes represents the 25<sup>th</sup> percentile. The lower horizontal line represents the lower adjacent value. Yellow dots and blue dots are outrange values.</p