Anti-LRP4 detection by immunoprecipitation of pools of sera.

<p>Supernatants from LRP4ecto HEK293T cells were immune-precipitated with the indicated pools (p) of sera that scored positive at FACS analysis: pool#1 and #2 from dSN-MG, Musk-MG pool and AChR-MG pool. Immuno-complexes were subdued to western blotting and probed with anti-c-Myc. Aliquots of total supernatants from EGFP-HEK293T and from LRP4ecto HEK293T cells were blotted alongside with immune-precipitates as negative and positive control, respectively. The specific, uppermost band is pointed by the arrow.</p

Anti-LRP4 detection by FACS analysis.

<p>Each of 101 MG s and 85 control sera was tested on both parental untransfected (shaded area) and on LRP4fl-HEK293T transfected cells (full line). None of the 45 NHS but only 14 MG sera showed a clear cut shift of mean fluorescence value with a ratio transfected/untransfected > 1.5. We show the immunoreactivity of one NHS, one dSN-MG (sample#8), one AChR-MG (sample#9) and one MuSK-MG serum (sample#14) (A,B,C,D respectively) as representative plots.</p

Anti-LRP4 immunoreactivity in 101 Italian myasthenic patients (MG) and controls.

<p>*:CI: Confidence Interval</p><p>Anti-LRP4 immunoreactivity in 101 Italian myasthenic patients (MG) and controls.</p

Expression of recombinant LRP4.

<p>(A) Flow cytofluorimetric analysis of parental untransfected HEK293T cells labeled with the rabbit anti-LRP4 antiserum (shaded area), compared to LRP4fl-HEK293T transfected cells (full line). (B) Precipitated supernatants from EGFP-HEK293T LRP4ecto-HEK293T cells were analyzed by anti-c-Myc immunoblotting; the band corresponding to the ecto-LRP4-myc tag fusion protein is indicated by arrow.</p