Genomic DNA extraction from sapwood of Pinus roxburghii for polymerase chain reaction studies

A method for extraction of genomic DNA from sapwood tissues of mature tall trees of Pinus roxburghii, where collection of needle tissues is extremely difficult has been standardized. The extracted DNA was comparable to that obtained from the needle tissue in terms of yield and purity. The yield of extracted DNA ranged from 6.98 to 19.668 μg / 100 mg tissue and A260 / A280 ratio ranged from 1.70 to 1.87. The polymerase chain reaction (PCR) amplification of the DNA extracted from sapwood tissue using random amplification of polymorphic DNA (RAPD), inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) markers was similar to that of DNA extracted from the needle tissues.Keywords: Pinus roxburghii, DNA extraction, sapwood, random amplification of polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), simple sequence repeat (SSR)African Journal of Biotechnology Vol. 12(15), pp. 1732-173

Molecular characterization of six populations of Acorus calamus L. using randomly amplified polymorphic DNA (RAPD) markers

Studies were undertaken to identify genetic relationship in six different populations of Acorus calamus L. through randomly amplified polymorphic DNA (RAPD) markers. A total of 574 DNA fragments ranging from 281 to 1353 bp were amplified using 10 selected primers. The number of amplification products produced by a primer ranged from as low as six to a maximum of 13, with an average of nine bands per primer. The cluster analysis revealed three major clusters; the first cluster contained sample collected from Lucknow and Paonta Uttaralkand, India. The second cluster had single sample from Bangalore India and the third cluster contained sample collected from Solan, Nauni and Hissar, India. The similarity coefficient value ranged from 0.97 to 0.88. The highest similarity coefficient (0.97) was detected between samples collected from Nauni and Hissar as well as between Solan and Hissar and the lowest (0.88) was detected between the pairs Lucknow and Bangalore, Lucknow and Solan, Bangalore and Solan. The level of polymorphism in our study was very low which showed that samples used for analysis would have close relationship.Key words: Randomly amplified polymorphic DNA (RAPD), similarity coefficient, polymorphism, Acorus calamus, primer