High resolution melt curves of a mutant DNA sample (<i>gyrA</i> D94G) serially diluted at concentrations of 100%, 50%, 25%, 12.5%, and 6.25%, and mixed with wildtype DNA.

<p>Wildtype samples are shown in black and samples with mutations are shown in color. Changes in melt curve shape demonstrating the presence of mutations were observed in samples with 100%, 50% and 25% mutant DNA. Experiments were performed in duplicate.</p

Primer sequences used for fluoroquinolone and streptomycin resistance HRM detection assays.

a<p>F: Forward, R: Reverse.</p>b<p>Nucleotide position is relative to the transcriptional start site of each gene.</p>c<p>Amplicon range of the HRM primers does not include the primer regions.</p

Primer sequences used to sequence <i>rpsL</i>, <i>rrs</i>, and <i>gyrA</i>.

a<p>Length, number of nucleotides.</p>b<p>%GC, number of G's and C's in the primer as a percentage of the total number of nucleotides.</p>c<p>F, forward; R, reverse.</p

Representative high resolution melt curves of (A) <i>gyrA</i> with Thr-95 control, (B) <i>gyrA</i> with Ser-95 control, (C) <i>rpsL</i>, and (D, E, F) <i>rrs</i> fragments 1, 2, 3 respectively, demonstrating the change in melt curve shape caused by mutations.

<p>Wildtype samples are shown in black and samples with mutations are shown in color. Experiments were performed in duplicate.</p

Sensitivity and specificity of the drug resistance detection HRM assays.

a<p>[Number of drug-resistant isolates with mutations]/[number of drug-resistant isolates with mutations+number of drug-resistant isolates without mutation].</p>b<p>Statistical calculations were performed with the free software available from <a href="http://www.measuringusability.com/wald.htm" target="_blank">http://www.measuringusability.com/wald.htm</a> using the Adjusted Wald method.</p>c<p>[Number of drug-susceptible isolates without mutations]/[number of drug-susceptible isolates with mutations+number of drug-susceptible isolates without mutation].</p

Difference in time to result between conventional culture-based method and the Verigene Test.

<p>^a Difference in time between BC-GP or BC-GN result and final culture-based identification and drug susceptibility test results</p><p>^b Only the isolates with concordant results from cultured-based method and the Verigene test were included for the time to result assessment.</p><p>Difference in time to result between conventional culture-based method and the Verigene Test.</p

Resistance gene profile in Gram negative organisms collected in this study.

<p>^a species identified by 16s Sequencing</p><p>Resistance gene profile in Gram negative organisms collected in this study.</p

Performance of BC-GP for identification of Gram positive organisms.

<p>^<i>a</i><i>95% confidence interval was calculated using Adjusted Wald Method (</i><a href="http://www.measuringu.com/wald.htm" target="_blank"><i>http</i>:<i>//www</i>.<i>measuringu</i>.<i>com/wald</i>.<i>htm</i></a>)</p><p>^b Misidentifed as <i>S</i>. <i>pneumoniae</i></p><p>Performance of BC-GP for identification of Gram positive organisms.</p

The overall concordance in bacterial identification between Verigene test and culture-based method in different study sites.

<p>QMH: Queen Mary Hospital; PMH: Princess Margaret Hospital; UCH: United Christian Hospital; PYNEH: Pamela Youde Nethersole Eastern Hospital; 95%CI: 95% confidence interval</p><p>^a 95% confidence interval was calculated using Adjusted Wald Method (<a href="http://www.measuringu.com/wald.htm" target="_blank">http://www.measuringu.com/wald.htm</a>)</p><p>The overall concordance in bacterial identification between Verigene test and culture-based method in different study sites.</p

Performance of Verigene Test in detection of drug resistant organisms.

<p>MRSA: Methicillin resistant <i>Staphylococcus aureus</i>; MRSE: Methicillin resistant <i>Staphylococcus epidermidis;</i> VRE: Vancomycin resistant Enterococcus; ESBL: Extended spectrum beta-lactamase; MDR <i>Acinetobacter</i>: Multidrug resisant <i>Acinetobacter</i></p><p>^a 95% confidence interval was calculated using Adjusted Wald Method (<a href="http://www.measuringu.com/wald.htm" target="_blank">http://www.measuringu.com/wald.htm</a>)</p><p>Performance of Verigene Test in detection of drug resistant organisms.</p