Gz, a guanine nucleotide-binding protein with unique biochemical properties

Cloning of a complementary DNA (cDNA) for Gz alpha, a newly appreciated member of the family of guanine nucleotide-binding regulatory proteins (G proteins), has allowed preparation of specific antisera to identify the protein in tissues and to assay it during purification from bovine brain. Additionally, expression of the cDNA in Escherichia coli has resulted in the production and purification of the recombinant protein. Purification of Gz from bovine brain is tedious, and only small quantities of protein have been obtained. The protein copurifies with the beta gamma subunit complex common to other G proteins; another 26- kDa GTP-binding protein is also present in these preparations. The purified protein could not serve as a substrate for NAD-dependent ADP- ribosylation catalyzed by either pertussis toxin or cholera toxin. Purification of recombinant Gz alpha (rGz alpha) from E. coli is simple, and quantities of homogeneous protein sufficient for biochemical analysis are obtained. Purified rGz alpha has several properties that distinguish it from other G protein alpha subunit polypeptides. These include a very slow rate of guanine nucleotide exchange (k = 0.02 min^-1), which is reduced greater than 20-fold in the presence of mM concentrations of Mg2+. In addition, the rate of the intrinsic GTPase activity of Gz alpha is extremely slow. The hydrolysis rate (kcat) for rGz alpha at 30 degrees C is 0.05 min^-1, or 200-fold slower than that determined for other G protein alpha subunits. rGz alpha can interact with bovine brain beta gamma but does not serve as a substrate for ADP-ribosylation catalyzed by either pertussis toxin or cholera toxin. These studies suggest that Gz may play a role in signal transduction pathways that are mechanistically distinct from those controlled by the other members of the G protein family

Measurables of CPCP Violation in Bd→DCP0KSB_d\to D^0_{CP}K_S at a BB-meson Factory

In the context of the standard electroweak model, we emphasize that $B_d\rightarrow D^0_{CP}K_S$ ($D^0_{CP}$ denotes a $CP$ eigenstate of $D^0$ or $\bar{D}^0$) can compete with $B_d\rightarrow \pi^+\pi^-$ in studying $CP$ violation and probing the Cabibbo-Kobayashi-Maskawa unitarity triangle. We discuss the measurables of direct and indirect $CP$ asymmetries in $B^0_d$ vs $\bar{B}^0_d\rightarrow D^0_{CP}K_S$ under the circumstance of an asymmetric $B$-meson factory running on the $\Upsilon(4S)$ resonance, and show that both the weak and strong phases are experimentally determinable even in the presence of unknown final-state interactions.Comment: 6 Postscript pages, accepted for publication in IL Nuovo Cimento A as a "Note Brevi

G protein beta gamma subunits synthesized in Sf9 cells. Functional characterization and the significance of prenylation of gamma

Heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) consist of a nucleotide-binding alpha subunit and a high- affinity complex of beta and gamma subunits. There is molecular heterogeneity of beta and gamma, but the significance of this diversity is poorly understood. Different G protein beta and gamma subunits have been expressed both singly and in combinations in Sf9 cells. Although expression of individual subunits is achieved in all cases, beta gamma subunit activity (support of pertussis toxin-catalyzed ADP-ribosylation of rGi alpha 1) is detected only when beta and gamma are expressed concurrently. Of the six combinations of beta gamma tested (beta 1 or beta 2 with gamma 1, gamma 2, or gamma 3), only one, beta 2 gamma 1, failed to generate a functional complex. Each of the other five complexes has been purified by subunit exchange chromatography using Go alpha-agarose as the chromatographic matrix. We have detected differences in the abilities of the purified proteins to support ADP- ribosylation of Gi alpha 1; these differences are attributable to the gamma component of the complex. When assayed for their ability to inhibit calmodulin-stimulated type-I adenylylcyclase activity or to potentiate Gs alpha-stimulated type-II adenylylcyclase, recombinant beta 1 gamma 1 and transducin beta gamma are approximately 10 and 20 times less potent, respectively, than the other complexes examined. Prenylation and/or further carboxyl-terminal processing of gamma are not required for assembly of the beta gamma subunit complex but are indispensable for high affinity interactions of beta gamma with either G protein alpha subunits or adenylylcyclases

An Improved Method of Potato Seed Treatment

Half of the seed potatoes that are planted each spring are infected with some plant disease. Black leg, Black scurf, Common scab, and Dry rots are the commonest of these diseases. They cut down the stand, injure the roots, mar the potatoes and reduce the yield. Illustration on cover shows the seed potatoes from a 2 1/2 bushel sack bought in the open market, sorted into two lots: those free from disease and those not free. Note that there are more diseased potatoes than healthy ones. \u27!\u27his is not an unusual case; in fact, it is somewhat better than the average. Such potatoes can be made into good seed by treating them, but seed treatment will not make good seed out of culls. The little potatoes and those badly diseased should be discarded for seed purposes

τ→ρππν\tau\to\rho\pi\pi\nu decays

Effective chiral theory of mesons is applied to study the four decay modes of $\tau\to\rho\pi\pi\nu$. Theoretical values of the branching ratios are in agreement with the data. The theory predicts that the $a_{1}$ resonance plays a dominant role in these decays. There is no new parameter in this study.Comment: 12 pages and one figur

Direct Syntheses of Amides From Long-Chained Aliphatic Amines

An examination has been made of direct procedures for the synthesis of amides. Dodecylamine, tetradecylamine, hexadecylamine and octadecylamine have been treated with a variety of aliphatic and aromatic acids. Direct heating of the amine and acid, in open containers and at elevated temperatures, gave quite satisfactory yields of amides. This direct procedure has been resolved into two stages. First, a mixture of amine and acid, dissolved or suspended in warm petroleum ether, gives promptly and in good yield the corresponding salt. These salts can be used for the characterization of amine and acid. Second, when the salts are heated they are converted to the amides. As an example, n-octadecyl amine and acetic acid gives a 94 per cent yield of salt melting at 84.5-85°. Upon heating this salt at 225° for fifteen minutes there is a quantitative conversion to n-octadecylacetamide (m.p., 78-78.5°)

epsilon'/epsilon in the Standard Model

In order to provide an estimate of eps'/eps several effective theories and physical effects have to be disentangled. In this talk I discuss how it is possible to predict eps '/eps taking into account all sources of large logs. The numerical result one obtains, \eps '/\eps \sim (1.7\pm 0.6) \cdot 10^{-4}, is in good agreement with present measurements.Comment: Talk presented at QCD2000, Montpellier july 200

Isospin Structure of Penguins And Their Consequences in BB Physics

Isospin structure of gluon mediated or strong penguin is significantly altered when the full electroweak corrections are included. This has the consequence that some previous analyses which relied on a simple isospin structure in charmless $B$ decays become inapplicable. We present the general Hamiltonian in next-to-leading order QCD, and illustrate our conclusion quantitatively for $B\rightarrow \pi\pi$ and $B\rightarrow K\pi$ decays in the factorization approximation. Some remarks on CP asymmetries in $B$ decays are also made.Comment: Revtex, 11 pages, typos in eq.(17) were correcte

Transition between nuclear and quark-gluon descriptions of hadrons and light nuclei

We provide a perspective on studies aimed at observing the transition between hadronic and quark-gluonic descriptions of reactions involving light nuclei. We begin by summarizing the results for relatively simple reactions such as the pion form factor and the neutral pion transition form factor as well as that for the nucleon and end with exclusive photoreactions in our simplest nuclei. A particular focus will be on reactions involving the deuteron. It is noted that a firm understanding of these issues is essential for unraveling important structure information from processes such as deeply virtual Compton scattering as well as deeply virtual meson production. The connection to exotic phenomena such as color transparency will be discussed. A number of outstanding challenges will require new experiments at modern facilities on the horizon as well as further theoretical developments.Comment: 37 pages, 17 figures, submitted to Reports on Progress in Physic