7 research outputs found

    A history of Indian immigration and settlement in Fiji

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    1v. and supplIn this thesis, the causes, nature and effects of Indian immigration and settlement in Fiji from 1879 to 1919 are studied from documentary and oral data. The subject is treated broadly from several angles, not analysed rigorously in relation to any single hypothesis. Indentured Indian immigrants were introduced into Fiji by the first Governor, Sir Arthur Gordon, who wished to obtain revenue from a limited commercial development of the colony without disrupting Fijian society. This object was successfully achieved but at the price of creating other problems for the future. Investment of Australian capital in the sugar industry ensured the continuance of the immigration. First two paragraphs of abstract)

    Ahmedabad : a study in Indian urban history

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    In what the author describes as a preliminary excursion into Indian urban history, he writes here not about Indian cities in general but about the unique experience of a particular city, Ahmedabad, the capital of Gujarat State in western India and for many years the home of Mahatma Gandhi and Sardar Patel. Ahmedabad, India's sixth city in size and one of the richest, was not a creation of British rule but an old center of trade and industry that adapted to the new age and became "The Manchester of India." Its recovery in the nineteenth century, after a decline in the previous one, contrasted with the experience of other Indian cities, many of which suffered a different fate under British rule. Ahmedabad provides an interesting qualification of many of the statements commonly made about Indian industrialization. Western influence and social change there were limited in the nineteenth century, and the early industrialization of the city was achieved within a society that remained socially and politically conservative. Modern Ahmedabad was the creation of Ahmedabadis, not of outsiders. In no other great city of India can the continuity of past and present be seen so clearly, and it is continuity rather than change that this book emphasizes. Mr. Gillion's account, focusing mainly on the century beginning with the British annexation in 1817, suggests a new way of viewing Indian social and economic history. Indirectly but none the less suggestively, this work reopens the whole question of westernization in India and places it in a fresh perspective

    The Fiji Indians : challenge to European dominance 1920-1946

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    Today the descendants of immigrants from India in Fiji outnumber the original Fijians. They are an integral and vital part of Fiji's multi-racial population. In this book, a sequel to the author's, Fiji's Indian Migrants, Dr Gillion deals with the very important period when there were strikes, boycotts and communal disputes as the Indian community sought dignity, identity and acceptance in its new home

    Limits of life in MgCl<sub>2</sub>-containing environments: chaotropicity defines the window

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    The biosphere of planet Earth is delineated by physico-chemical conditions that are too harsh for, or inconsistent with, life processes and maintenance of the structure and function of biomolecules. To define the window of life on Earth (and perhaps gain insights into the limits that life could tolerate elsewhere), and hence understand some of the most unusual biological activities that operate at such extremes, it is necessary to understand the causes and cellular basis of systems failure beyond these windows. Because water plays such a central role in biomolecules and bioprocesses, its availability, properties and behaviour are among the key life-limiting parameters. Saline waters dominate the Earth, with the oceans holding 96.5% of the planet's water. Saline groundwater, inland seas or saltwater lakes hold another 1%, a quantity that exceeds the world's available freshwater. About one quarter of Earth's land mass is underlain by salt, often more than 100 m thick. Evaporite deposits contain hypersaline waters within and between their salt crystals, and even contain large subterranean salt lakes, and therefore represent significant microbial habitats. Salts have a major impact on the nature and extent of the biosphere, because solutes radically influence water's availability (water activity) and exert other activities that also affect biological systems (e.g. ionic, kosmotropic, chaotropic and those that affect cell turgor), and as a consequence can be major stressors of cellular systems. Despite the stressor effects of salts, hypersaline environments can be heavily populated with salt-tolerant or -dependent microbes, the halophiles. The most common salt in hypersaline environments is NaCl, but many evaporite deposits and brines are also rich in other salts, including MgCl2 (several hundred million tonnes of bischofite, MgCl2·6H2O, occur in one formation alone). Magnesium (Mg) is the third most abundant element dissolved in seawater and is ubiquitous in the Earth's crust, and throughout the Solar System, where it exists in association with a variety of anions. Magnesium chloride is exceptionally soluble in water, so can achieve high concentrations (> 5 M) in brines. However, while NaCl-dominated hypersaline environments are habitats for a rich variety of salt-adapted microbes, there are contradictory indications of life in MgCl2-rich environments. In this work, we have sought to obtain new insights into how MgCl2 affects cellular systems, to assess whether MgCl2 can determine the window of life, and, if so, to derive a value for this window. We have dissected two relevant cellular stress-related activities of MgCl2 solutions, namely water activity reduction and chaotropicity, and analysed signatures of life at different concentrations of MgCl2 in a natural environment, namely the 0.05-5.05 M MgCl2 gradient of the seawater: hypersaline brine interface of Discovery Basin - a large, stable brine lake almost saturated with MgCl2, located on the Mediterranean Sea floor. We document here the exceptional chaotropicity of MgCl2, and show that this property, rather than water activity reduction, inhibits life by denaturing biological macromolecules. In vitro, a test enzyme was totally inhibited by MgCl 2 at concentrations below 1 M; and culture medium with MgCl 2 concentrations above 1.26 M inhibited the growth of microbes in samples taken from all parts of the Discovery interface. Although DNA and rRNA from key microbial groups (sulfate reducers and methanogens) were detected along the entire MgCl2 gradient of the seawater: Discovery brine interface, mRNA, a highly labile indicator of active microbes, was recovered only from the upper part of the chemocline at MgCl2 concentrations of less than 2.3 M. We also show that the extreme chaotropicity of MgCl 2 at high concentrations not only denatures macromolecules, but also preserves the more stable ones: such indicator molecules, hitherto regarded as evidence of life, may thus be misleading signatures in chaotropic environments. Thus, the chaotropicity of MgCl2 would appear to be a window-of-life-determining parameter, and the results obtained here suggest that the upper MgCl2 concentration for life, in the absence of compensating (e.g. kosmotropic) solutes, is about 2.3 M. © Journal compilation © 2007 Society for Applied Microbiology and Blackwell Publishing Ltd

    Concomitant osmotic and chaotropicity-induced stresses in Aspergillus wentii: compatible solutes determine the biotic window

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    Whereas osmotic stress response induced by solutes has been well-characterized in fungi, less is known about the other activities of environmentally ubiquitous substances. The latest methodologies to define, identify and quantify chaotropicity, i.e. substance-induced destabilization of macromolecular systems, now enable new insights into microbial stress biology (Cray et al. in Curr Opin Biotechnol 33:228–259, 2015a, doi:10.1016/j.copbio.2015.02.010; Ball and Hallsworth in Phys Chem Chem Phys 17:8297–8305, 2015, doi:10.1039/C4CP04564E; Cray et al. in Environ Microbiol 15:287–296, 2013a, doi:10.1111/1462-2920.12018). We used Aspergillus wentii, a paradigm for extreme solute-tolerant fungal xerophiles, alongside yeast cell and enzyme models (Saccharomyces cerevisiae and glucose-6-phosphate dehydrogenase) and an agar-gelation assay, to determine growth-rate inhibition, intracellular compatible solutes, cell turgor, inhibition of enzyme activity, substrate water activity, and stressor chaotropicity for 12 chemically diverse solutes. These stressors were found to be: (i) osmotically active (and typically macromolecule-stabilizing kosmotropes), including NaCl and sorbitol; (ii) weakly to moderately chaotropic and non-osmotic, these were ethanol, urea, ethylene glycol; (iii) highly chaotropic and osmotically active, i.e. NH4NO3, MgCl2, guanidine hydrochloride, and CaCl2; or (iv) inhibitory due primarily to low water activity, i.e. glycerol. At ≤0.974 water activity, Aspergillus cultured on osmotically active stressors accumulated low-Mr polyols to ≥100 mg g dry weight−1. Lower-Mr polyols (i.e. glycerol, erythritol and arabitol) were shown to be more effective for osmotic adjustment; for higher-Mr polyols such as mannitol, and the disaccharide trehalose, water-activity values for saturated solutions are too high to be effective; i.e. 0.978 and 0.970 (25 ºC). The highly chaotropic, osmotically active substances exhibited a stressful level of chaotropicity at physiologically relevant concentrations (20.0–85.7 kJ kg−1). We hypothesized that the kosmotropicity of compatible solutes can neutralize chaotropicity, and tested this via in-vitro agar-gelation assays for the model chaotropes urea, NH4NO3, phenol and MgCl2. Of the kosmotropic compatible solutes, the most-effective protectants were trimethylamine oxide and betaine; but proline, dimethyl sulfoxide, sorbitol, and trehalose were also effective, depending on the chaotrope. Glycerol, by contrast (a chaotropic compatible solute used as a negative control) was relatively ineffective. The kosmotropic activity of compatible solutes is discussed as one mechanism by which these substances can mitigate the activities of chaotropic stressors in vivo. Collectively, these data demonstrate that some substances concomitantly induce chaotropicity-mediated and osmotic stresses, and that compatible solutes ultimately define the biotic window for fungal growth and metabolism. The findings have implications for the validity of ecophysiological classifications such as ‘halophile’ and ‘polyextremophile’; potential contamination of life-support systems used for space exploration; and control of mycotoxigenic fungi in the food-supply chain
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