Immunofluorescence staining of neutrophils by anti-PTX3 Abs.

<p>Slides containing 4 biochips as substrate ethanol-fixed (upper left), formol-aceton fixed neutrophils (upper right) methanol-fixed neutrophils (lower left) and HEp2 cells (lower right) were incubated with <b>A</b>, a serum from a patient with anti-PTX3 aAbs and neither MPO, PR3, BPI, lactoferrine, elastase nor cathepsin G ANCA, <b>B</b>, an anti-PTX3 polyclonal antibody or <b>C-E</b>, control sera for p-ANCA (<b>C</b>), a-ANCA (<b>D)</b>, c-ANCA (<b>E</b>). <b>A, C-E</b>, results are representative of the results obtained with one out of four sera. B, Results are representative of one out of three experiments.</p

Longitudinal analysis of anti-PTX3 aAbs.

<p>Anti-PTX3 aAbs were analysed by ELISA in 14 patients with active disease (onset or relapse) and at remission. Results are expressed in OD values. Dotted line corresponds to mean + 2 SD titres of anti-PTX3 aAbs in HS sera; full line corresponds to the mean OD in each group of patients. Each line represents one patient. *p<0.05.</p