Nitrous Oxide Flux from Poultry-Manured Erosion Plots and Grass Filters after Simulated Rain

Adding carbon-rich materials to fields, like manure, may enhance denitrification. Grass filters, which are used to trap surface runoff from these fields, may also provide a carbon-rich environment that favors water infiltration and denitrification. Nitrous oxide (N2O) may be evolved these settings. It is a radiatively important trace gas and intermediate in the denitrification pathway and several other microbial processes. We measured N2O flux, after simulated rain, using a soil cover technique in poultry-manured plots and grass filters receiving their runoff. Intact soil cores were used to relate the N2O flux to the denitrification potential of the plots. Nitrous oxide fluxes were smaller in grass filters than in manured plots, even though more denitrifying bacteria were present. The average N2O flux in the three most dynamic erosion plots was 755 µg N2O-N m−2h−1, which was 39% of the maximal denitrification rate measured in acetylene-blocked, NO−3-amended soil cores. Nitrous oxide flux immediately after rainfall was greater than N2O flux measurements reported for similar agricultural settings

Trapping Fecal Bacteria and Sediment in Surface Runoff From Cropland Treated With Poultry Litter

Between 1991 and 1994 the broiler population exploded in Kentucky as the poultry industry began to expand. The Kentucky Department of Agriculture predicts that within four years annual broiler production could exceed 275 million birds. This may be good for Kentucky\u27s economy but it carries some important environmental consequences. If expansion continues as anticipated, the estimated waste production from broilers for processing could reach 300,000 tons per year (assuming each broiler house produces 150,000 birds per year and the yearly manure and litter production per house is approximately 160 tons)

Filter Strip Length and Fecal Bacteria Trapping from Poultry Waste - An Update

Cheap, efficient, and environmentally sound waste disposal will be needed as Kentucky\u27s broiler industry expands. The filter strip length needed to protect water resources from contaminants in surface runoff is a pressing issue in waste management and water quality. In a previous Soil Science News and Views (Vol. 15, No. 8) we reported that grass filter strips as short as 15 feet can trap over 90% of the fecal bacteria eroding from land-applied and incorporated poultry waste during runoff following rainstorms. In this update, we provide some additional information and conclusions from that study on filter strip length, based on comparisons of filter strips receiving equal amounts of surface runoff

Nitrous Oxide Flux from Poultry-Manured Erosion Plots and Grass Filters after Simulated Rain

Adding carbon-rich materials to fields, like manure, may enhance denitrification. Grass filters, which are used to trap surface runoff from these fields, may also provide a carbon-rich environment that favors water infiltration and denitrification. Nitrous oxide (N2O) may be evolved these settings. It is a radiatively important trace gas and intermediate in the denitrification pathway and several other microbial processes. We measured N2O flux, after simulated rain, using a soil cover technique in poultry-manured plots and grass filters receiving their runoff. Intact soil cores were used to relate the N2O flux to the denitrification potential of the plots. Nitrous oxide fluxes were smaller in grass filters than in manured plots, even though more denitrifying bacteria were present. The average N2O flux in the three most dynamic erosion plots was 755 µg N2O-N m−2h−1, which was 39% of the maximal denitrification rate measured in acetylene-blocked, NO−3-amended soil cores. Nitrous oxide flux immediately after rainfall was greater than N2O flux measurements reported for similar agricultural settings

Persistence of antibiotic resistance genes in beef cattle backgrounding environment over two years after cessation of operation.

Confined animal feeding operations can facilitate the spread of genes associated with antibiotic resistance. It is not known how cattle removal from beef cattle backgrounding operation affects the persistence of antibiotic resistance genes (ARGs) in the environment. We investigated the effect of cessation of beef cattle backgrounding operation on the persistence and distribution of ARGs in the beef cattle backgrounding environment. The study was conducted at a pasture-feedlot type beef cattle backgrounding operation which consisted of feeding and grazing areas that were separated by a fence with an access gate. Backgrounding occurred for seven years before cattle were removed from the facility. Soil samples (n = 78) from 26 georeferenced locations were collected at the baseline before cattle were removed, and then one year and two years after cattle were removed. Metagenomic DNA was extracted from the soil samples and total bacterial population (16S rRNA), total Enterococcus species and class 1 integrons (intI1), and erythromycin (ermB and ermF), sulfonamide (sul1 and sul2) and tetracycline (tetO, tetW and tetQ) resistance genes were quantified. Concentrations of total bacteria, Enterococcus spp., class 1 integrons, and ARGs were higher in the feeding area and its immediate vicinity (around the fence and the gate) followed by a gradient decline along the grazing area. Although the concentrations of total bacteria, Enterococcus spp., class 1 integrons and ARGs in the feeding area significantly decreased two years after cattle removal, their concentrations were still higher than that observed in the grazing area. Higher concentrations over two years in the feeding area when compared to the grazing area suggest a lasting effect of confined beef cattle production system on the persistence of bacteria and ARGs in the soil