Proteomic analysis in wild type and cisplatin resistant yeast strain of saccharomyces cerevisiae

Research carried out in oncology has allowed the identification and characterization of molecular markers of resistance to antineoplastic drugs. It supposes a great advance and allows the administration of customized chemotherapies to patients. The aim of this work is to carry out a proteomic analysis in the wild type strain and cisplatin resistant strain of Saccharomyces cerevisiae in order to look for possible markers of resistance, as well as the mechanisms involved.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

One hundred of cells generations connect the resistance to drugs and the replicative aging mechanisms

There are researchs that reveal a relationship between acquired resistance to antineoplastic drugs and aging. Therefore, the identification of genes involved in senescence could have a potential therapeutic utility for the prediction and/or prevention of resistance to antineoplastic drugs. The aim of this work is to study the relationship between replicative aging and acquired resistance to Cisplatin and Bleomycin in the yeast Saccharomyces cerevisiae.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

New markers of bleomycin resistance

Resistance to chemotherapy is one of the main problems of this type of therapy. Studies carried out determine the usefulness of predictive biomarkers to improve this treatment. The aim of this work is to carry out a proteomic analysis in the wild type strain and bleomycin resistant strain of Saccharomyces cerevisiae in order to look for possible markers of resistance, as well as the mechanisms involved.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Mechanisms of cross resistance between cisplatin and bleomycin in saccharomyces cerevisiae

Tumor cells can simultaneously acquire resistance against a diverse group of drugs. This phenomenon is called pleotropic resistance or multidrug resistance. This mechanism could explain the tumor resistance observed in chemotherapy treatments with multiple agents. The aim of this work is to study possible mechanisms of cross resistance between Cisplatin and Bleomycin in Saccharomyces cerevisiae.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Resistance to bleomycin increases the chronological life of cells

The identification of genes involved in chronological aging could have a potential utility as molecular markers in the chemotherapy treatment of cancer. The aim of this work is to study the relationship between the chronological aging and the resistance to Bleomycin for the purpose of establish the basic interactions between these phenomenons for further investigation of molecular markers.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Resistance acquisition after seven cycles of in vitro bleomycin exposure

Bleomycin is an antineoplastic drug that causes DNA damage by intra- and inter-strand crosslink and DNA strand breaks. This drug is used to treat several types of cancer. The aim of this work is to study the effect of Bleomycin on S. cerevisiae cells and select bleomycin-resistant cells to study the process of resistance acquisition to establish new protocols in in vitro drugs exposure.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Relationship between chronological aging and acquired resistance to cisplatin in the yeast saccharomyces cerevisiae

The Sir2 gene is associated with an increase in longevity in yeasts, worms, flies and rodents. The human homolog, Sirt1, is also involved in longevity, by inhibiting cellular senescence. Studies carry out in tumor cells of neuroblastoma, osteosarcoma, breast and ovary, resistant and sensitive to antineoplastic drugs, suggest that the increase of Sirt1 expression may represent a general phenomenon associated with resistance to chemotherapy, independent of cell type or drug used to induce resistance. Thus, after analyzing tumor biopsies after treating patients with chemotherapy, high amounts of Sirt1 have been observed. The aim of this work is to study the relationship between chronological aging and acquired resistance to Cisplatin in the yeast Saccharomyces cerevisiae.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Selection of resistance to cisplatin in saccharomyces cerevisiae as a model for antineoplastic drugs studies

The budding yeast (S. cerevisiae) is an excellent eukaryotic model to study the antineoplastic drugs effects, due to the well-characterized metabolic and genetic characteristics and the conserved similarity in molecular mechanisms with other species including human cells. The aim of this work is to study the effect of cisplatin on S. cerevisiae cells and select cisplatin-resistant cells of this organism as a model of study of resistance to antineoplastic drugs.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Intestinal microbiota and colorectal cancer

Colorectal cancer may be influenced by changes in the intestinal microbiota that affect the mucosa and cause an immune response capable of producing inflammatory effects. Although there are still few studies in this regard, it is necessary to emphasize the need to expand the studies on this topic and to state the usefulness of the new technologies based on metagenomics.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Case study of the intestinal microbiota using the XGN-MBI metagenomic assay

The microbiota of the colon and rectum is the most abundant and diverse of the human body, with a density of up to 1-2 kg of weight and a diversity that exceeds a thousand species. Metagenomics can be defined as the application of modern genomic techniques for the direct study of communities of microorganisms in their natural environment The aim of this study is to prove the usefulness of new technologies based on metagenomics, such as the XGN-MBI assay to realize gut microbiota studies.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech