A Preliminary Quantification of the Impacts of Aspen to Conifer Succession on Water Yield Within the Colorado River Basin (A Process Aggravating the Salt Pollution Problem)

Heat pulse velocity techniques were developed for effectively monitoring water movement in aspen (Populus ttremuloides), subalpine fir (Abies lasiocarpa), and Englemann spruce (Picea engelmannii). Once the techniques were perfected, transpiration was monitored in replicated trees of each species for one year. This data was used to modify the plant activity index and the crop coefficient for each species within the model ASPCON, a deterministic, lumped-parameter model describing the hydrology of aspen to conifer succession. Results of the modeling indicate 18.5 cm(7.3 in) net loss of moisture available for stream flow when spruce replace aspen, and a loss of 7.1 cm (2.8 in) when fir forests cover the watershed. The aspen to conifer successional trend is therefore significantly reducing water yields within the Colorado River Basin, water that could be used to dilute salt downstream from the high water-yielding watersheds

Fecal Coliform Release Studies and Development of a Preliminary Nonpoint Source Transport Model for Indicator Bacteria

The effect of grazing on water quality has been documented by bacteriological studies of streams adjacent to grazed areas. Bacterial release from fecal deposits is a parameter of the pollution transport mechanism that is poorly understood. The objective of this study was to determine a fecal coliform release function for cattle fecal deposits. Standard cowpies were rained on with a rainfall simulator, and the fecal coliform counts were determined using the most probably number (MPN) method of enumeration. The fecal deposits were rained on at ages 2 through 100 days. The effects of rainfall intensity and recurrent rainfall were tested. Naturally occurring fecal deposits were also tested to compare their results with the results from the standard cowpies. A log-log regression was found to describe the decline in peak fecal coliform release with fecal deposit age. The 100-day-old fecal deposits produced peak counts of 4,200 fecal coliforms per 100 milliliters of water. This quantity of release is insignificant compared to the release from fresher fecal material. Rainfall intensity had little effect on peak fecal coliform release from fecal deposits that ere 2 or 10 days old. At age 20 days the effect of rainfall intensity was significant; the highest intensity gave the lowest peak counts, and the lowest intensity gave the highest peak counts. The effect of rainfall intensity appears to be related to the dryness of the fecal deposits. Peak fecal coliform counts were significantly lowered by raining on the fecal deposits more than once. This decline was thought to be produced by the loss of bacteria from the fecal deposits during the previous wettings. Standard cowpies produced a peak release regression that was not significantly different from the regression for the natural fecal deposits. Apparently, grossly manipulating the fecal deposits did not significantly change the release patterns

Control of Transcription by Cell Size

Cell size increases significantly with increasing ploidy. Differences in cell size and ploidy are associated with alterations in gene expression, although no direct connection has been made between cell size and transcription. Here we show that ploidy-associated changes in gene expression reflect transcriptional adjustment to a larger cell size, implicating cellular geometry as a key parameter in gene regulation. Using RNA-seq, we identified genes whose expression was altered in a tetraploid as compared with the isogenic haploid. A significant fraction of these genes encode cell surface proteins, suggesting an effect of the enlarged cell size on the differential regulation of these genes. To test this hypothesis, we examined expression of these genes in haploid mutants that also produce enlarged size. Surprisingly, many genes differentially regulated in the tetraploid are identically regulated in the enlarged haploids, and the magnitude of change in gene expression correlates with the degree of size enlargement. These results indicate a causal relationship between cell size and transcription, with a size-sensing mechanism that alters transcription in response to size. The genes responding to cell size are enriched for those regulated by two mitogen-activated protein kinase pathways, and components in those pathways were found to mediate size-dependent gene regulation. Transcriptional adjustment to enlarged cell size could underlie other cellular changes associated with polyploidy. The causal relationship between cell size and transcription suggests that cell size homeostasis serves a regulatory role in transcriptome maintenance.National Institutes of Health (U.S.) (grant GM035010)National Institutes of Health (U.S.) (grant GM040266

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Apparent Sap Velocities in Big Sagebrush

Peak daily sap velocities were rather consistent throughout the year, and were always less than five cm/hr (average of five plants). A 30-variable multiple regression equation involving environmental parameters measured near plants accounted for only 54.05% of variability associated with apparent sap velocity measurements. Big sagebrush plants must, therefore, exert considerable physiological control of transpirational water losses.This material was digitized as part of a cooperative project between the Society for Range Management and the University of Arizona Libraries.The Journal of Range Management archives are made available by the Society for Range Management and the University of Arizona Libraries. Contact [email protected] for further information.Migrated from OJS platform August 202

Fecal Coliform Release Patterns from Fecal Material of Cattle

The study examined the magnitude of a release of indicator bacteria (fecal coliform) from bovine fecal deposits that were rained on by a rainfall simulator at a rate of 6.1 ± 0.3 cm/h for 15 min, as affected by duration of rainfall and age of fecal deposits. Standard fecal deposits were placed on a platform and rained on with the runoff water being sampled at 5, 10, and 15 min. Samples were then examined by the most probable number (MPN) method for the presence of fecal coliforms. Results indicate the potential for bacterial pollution from bovine fecal deposits. An equilibrium in the concentration of fecal coliforms being released from the fecal deposit was reached within 10 min. Fecal deposits \u3c 5 d of age released fecal coliforms on the order of millions/100 mL of water. Concentrations declined to 40,000/100 mL at 30 d of not-rained on age. The decline followed a typical bacterial death curve