Molecular epidemiology of African sleeping sickness

Human sleeping sickness in Africa, caused by Trypanosoma brucei spp. raises a number of questions. Despite the widespread distribution of the tsetse vectors and animal trypanosomiasis, human disease is only found in discrete foci which periodically give rise to epidemics followed by periods of endemicity A key to unravelling this puzzle is a detailed knowledge of the aetiological agents responsible for different patterns of disease--knowledge that is difficult to achieve using traditional microscopy. The science of molecular epidemiology has developed a range of tools which have enabled us to accurately identify taxonomic groups at all levels (species, subspecies, populations, strains and isolates). Using these tools, we can now investigate the genetic interactions within and between populations of Trypanosoma brucei and gain an understanding of the distinction between human- and nonhuman-infective subspecies. In this review, we discuss the development of these tools, their advantages and disadvantages and describe how they have been used to understand parasite genetic diversity, the origin of epidemics, the role of reservoir hosts and the population structure. Using the specific case of T.b. rhodesiense in Uganda, we illustrate how molecular epidemiology has enabled us to construct a more detailed understanding of the origins, generation and dynamics of sleeping sickness epidemics

Turbulence and turbulent mixing in natural fluids

Turbulence and turbulent mixing in natural fluids begins with big bang turbulence powered by spinning combustible combinations of Planck particles and Planck antiparticles. Particle prograde accretions on a spinning pair releases 42% of the particle rest mass energy to produce more fuel for turbulent combustion. Negative viscous stresses and negative turbulence stresses work against gravity, extracting mass-energy and space-time from the vacuum. Turbulence mixes cooling temperatures until strong-force viscous stresses freeze out turbulent mixing patterns as the first fossil turbulence. Cosmic microwave background temperature anisotropies show big bang turbulence fossils along with fossils of weak plasma turbulence triggered as plasma photon-viscous forces permit gravitational fragmentation on supercluster to galaxy mass scales. Turbulent morphologies and viscous-turbulent lengths appear as linear gas-proto-galaxy-clusters in the Hubble ultra-deep-field at z~7. Proto-galaxies fragment into Jeans-mass-clumps of primordial-gas-planets at decoupling: the dark matter of galaxies. Shortly after the plasma to gas transition, planet-mergers produce stars that explode on overfeeding to fertilize and distribute the first life.Comment: 23 pages 12 figures, Turbulent Mixing and Beyond 2009 International Center for Theoretical Physics conference, Trieste, Italy. Revision according to Referee comments. Accepted for Physica Scripta Topical Issue to be published in 201

Leadership in a Turnaround Situation and a Multicultural Environment

Leadership style in a turnaround situation, especially in a multicultural environment, is very important to the success of the organization. As the organization is transformed to a successful one, the leader may have to adapt his or her style. This case focuses on the leadership of a new CEO recruited to overcome a crisis situation in a small community bank. The CEO also faced the additional challenges of being from a different culture than most of his employees and of interacting with the founder and previous CEO who had quite a different leadership style. In the process of turning the bank around and implementing a new strategy, the new CEO also transformed his leadership style in order to reduce anxiety and gain the respect and confidence of the employees, the board of directors, and the community

The influence of the preparation method of NiOx photocathodes on the efficiency of p-type dye-sensitised solar cells

Improving the efficiency of p-type dye-sensitized solar cells (DSCs) is an important part of the development of high performance tandem DSCs. The optimization of the conversion efficiency of p-DSCs could make a considerable contribution in the improvement of solar cells at a molecular level. Nickel oxide is the most widely used material in p-DSCs, due to its ease of preparation, chemical and structural stability, and electrical properties. However, improvement of the quality and conductivity of NiO based photocathodes needs to be achieved to bring further improvements to the solar cell efficiency. The subject of this review is to consider the effect of the preparation of NiO surfaces on their efficiency as photocathodes. (C) 2015 Elsevier B.V. All rights reserved

A reconfigurable real-time morphological system for augmented vision

There is a significant number of visually impaired individuals who suffer sensitivity loss to high spatial frequencies, for whom current optical devices are limited in degree of visual aid and practical application. Digital image and video processing offers a variety of effective visual enhancement methods that can be utilised to obtain a practical augmented vision head-mounted display device. The high spatial frequencies of an image can be extracted by edge detection techniques and overlaid on top of the original image to improve visual perception among the visually impaired. Augmented visual aid devices require highly user-customisable algorithm designs for subjective configuration per task, where current digital image processing visual aids offer very little user-configurable options. This paper presents a highly user-reconfigurable morphological edge enhancement system on field-programmable gate array, where the morphological, internal and external edge gradients can be selected from the presented architecture with specified edge thickness and magnitude. In addition, the morphology architecture supports reconfigurable shape structuring elements and configurable morphological operations. The proposed morphology-based visual enhancement system introduces a high degree of user flexibility in addition to meeting real-time constraints capable of obtaining 93 fps for high-definition image resolution

Increasing trap stiffness with position clamping in holographic optical tweezers

We present a holographic optical tweezers system capable of position clamping multiple particles. Moving an optical trap in response to the trapped object's motion is a powerful technique for optical control and force measurement. We have now realised this experimentally using a Boulder Nonlinear Systems Spatial Light Modulator (SLM) with a refresh rate of 203Hz. We obtain a reduction of 44% in the variance of the bead's position, corresponding to an increase in effective trap stiffness of 77%. This reduction relies on the generation of holograms at high speed. We present software capable of calculating holograms in under 1ms using a graphics processor unit. © 2009 Optical Society of America

Top-Down Fragmentation of a Warm Dark Matter Filament

We present the first high-resolution n-body simulations of the fragmentation of dark matter filaments. Such fragmentation occurs in top-down scenarios of structure formation, when the dark matter is warm instead of cold. In a previous paper (Knebe et al. 2002, hereafter Paper I), we showed that WDM differs from the standard Cold Dark Matter (CDM) mainly in the formation history and large-scale distribution of low-mass haloes, which form later and tend to be more clustered in WDM than in CDM universes, tracing more closely the filamentary structures of the cosmic web. Therefore, we focus our computational effort in this paper on one particular filament extracted from a WDM cosmological simulation and compare in detail its evolution to that of the same CDM filament. We find that the mass distribution of the halos forming via fragmentation within the filament is broadly peaked around a Jeans mass of a few 10^9 Msun, corresponding to a gravitational instability of smooth regions with an overdensity contrast around 10 at these redshifts. Our results confirm that WDM filaments fragment and form gravitationally bound haloes in a top-down fashion, whereas CDM filaments are built bottom-up, thus demonstrating the impact of the nature of the dark matter on dwarf galaxy properties.Comment: 7 pages, 7 figures, replaced with MNRAS accepted version (minor revisions