The Effect Of Cadmium on Sperm Quality and Fertilization Of Cyprinus carpio L.

The objective of the study was to determine the effect of cadmium on sperm quality and fertilization of C. carpio L. Sperm and eggs were collected by abdomen striping from the mature testis and ovary of C. carpio L. This study used one control and four treatment groups of variation on the cadmium concentration (0, 50, 100, 150, and 200 ppm) with eight replications. Sperm motility (mass motility, mass motility duration, and individual motility duration) and viability were measured after three to four seconds of incubation in the water. The percentage of fertility success was calculated by observing embryo development after the eggs were mixed with sperm and incubated in the water for 72 hours. The success of the fertilization process was indicated by a color change of the egg that darkens after successful fertilization, and white-milk if failed. The data were analyzed using analysis of variance (α = 0.05). The results of this study indicate that exposure of 50 ppm cadmium and control group shown success in term of sperm quality (motility and viability) and fertilization, but at 100 ppm or more decreased the sperm quality and fertilization rate. It can be concluded that cadmium exposure decreases sperm quality and fertility at 100 ppm or higher concentrations

The Effect of Cadmium on Sperm Quality and Fertilization of Cyprinus carpio L.

The objective of the study was to determine the effect of cadmium on sperm quality and fertilization of C. carpio L. Sperm and eggs were collected by abdomen striping from the mature testis and ovary of C. carpio L. This study used one control and four treatment groups of variation on the cadmium concentration (0, 50, 100, 150, and 200 ppm) with eight replications. Sperm motility (mass motility, mass motility duration, and individual motility duration) and viability were measured after three to four seconds of incubation in the water. The percentage of fertility success was calculated by observing embryo development after the eggs were mixed with sperm and incubated in the water for 72 hours. The success of the fertilization process was indicated by a color change of the egg that darkens after successful fertilization, and white-milk if failed. The data were analyzed using analysis of variance (α = 0.05). The results of this study indicate that exposure of 50 ppm cadmium and control group shown success in term of sperm quality (motility and viability) and fertilization, but at 100 ppm or more decreased the sperm quality and fertilization rate. It can be concluded that cadmium exposure decreases sperm quality and fertility at 100 ppm or higher concentrations

The Effect Of Cadmium on Sperm Quality and Fertilization Of Cyprinus carpio L.

The objective of the study was to determine the effect of cadmium on sperm quality and fertilization of C. carpio L. Sperm and eggs were collected by abdomen striping from the mature testis and ovary of C. carpio L. This study used one control and four treatment groups of variation on the cadmium concentration (0, 50, 100, 150, and 200 ppm) with eight replications. Sperm motility (mass motility, mass motility duration, and individual motility duration) and viability were measured after three to four seconds of incubation in the water. The percentage of fertility success was calculated by observing embryo development after the eggs were mixed with sperm and incubated in the water for 72 hours. The success of the fertilization process was indicated by a color change of the egg that darkens after successful fertilization, and white-milk if failed. The data were analyzed using analysis of variance (α = 0.05). The results of this study indicate that exposure of 50 ppm cadmium and control group shown success in term of sperm quality (motility and viability) and fertilization, but at 100 ppm or more decreased the sperm quality and fertilization rate. It can be concluded that cadmium exposure decreases sperm quality and fertility at 100 ppm or higher concentrations

PENGARUH TOKSISITAS KADMIUM TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA SECARA IN VITRO SERTA FERTILITAS IKAN MAS (Cyprinus carpio L.)

Penelitian ini bertujuan untuk mengetahui pengaruh toksisitas kadmium terhadap motilitas dan viabilitas spermatozoa secara in vitro serta fertilitas ikan Mas (Cyprinus carpio L.). Penelitian ini menggunakan spermatozoa dan sel telur dari ikan Mas yang matang gonad. Penelitian ini menggunakan satu kelompok kontrol dan empat kelompok perlakuan dengan variasi konsentrasi Kadmium (50, 100, 150, 200 ppm) serta pengulangan sebanyak delapan kali. Pengambilan data motilitas massa menggunakan skor, sedangkan untuk durasi motilitas adalah detik. Data yang telah didapatkan dianalisis dengan uji varian satu arah (One Way ANOVA), uji Duncan (α=0.05) dan uji Brown Forshyte (α˃0.05). Hasil penelitian ini menunjukkan bahwa tingkat kualitas spermatozoa dan keberhasilan fertilitas pada kelompok kontrol tidak berbeda signifikan dengan kelompok perlakuan Cd dengan konsentrasi 50 ppm, sedangkan penurunan kualitas spermatozoa dan fertilitas terjadi pada konsentrasi kadmium 100 ppm atau lebih. Kesimpulan dari penelitian ini adalah terdapat pengaruh penurunan kualitas spermatozoa dan penurunan fertilitas ikan Mas akibat paparan kadmium pada konsentrasi 100 ppm atau lebih