ASSESSMENT OF THE POTENTIAL FOR DEVELOPMENT OF AGRITOURISM IN BINH DINH PROVINCE, VIETNAM

This study evaluates the potential for agritourism development in Binh Dinh Province, Central Vietnam. The analytical hierarchical process is used to determine the weights of four factors with 25 measurement criteria to assess agritourism potential. The research results show that Binh Dinh Province has great agritourism potential based on four evaluation factors, including (1) local agricultural tourism resources, (2) the trend for agritourism development and the agritourism market, (3) the local community’s willingness, and (4) the supporting activities of the local government. In particular, the local agricultural tourism resources factor received the highest score, followed by the local community’s willingness and the supporting activities of the local government. The trend for agritourism development and the agritourism market was rated the lowest. Therefore, Binh Dinh has viable opportunities to encourage the growth of agritourism to improve farmers’ livelihoodsDu lịch nông nghiệp đem lại nhiều cơ hội cho phát triển kinh tế cũng như đa dạng hóa các loại hình du lịch và cải thiện đời sống sinh kế của người dân. Nghiên cứu sử dụng phương pháp phân tích thứ bậc (AHP) để tính các trọng số của các tiêu chí đánh giá tiềm năng phát triển du lịch nông nghiệp tại các huyện, thị xã phía bắc tỉnh Bình Định. Tiếp theo, kết quả mô hình hóa sơ đồ mạng cho thấy cả 4 địa phương An Lão, Hoài Ân, Phù Mỹ, thị xã Hoài Nhơn đều được đánh giá ở mức điểm cao về tiềm năng phát triển du lịch nông nghiệp trên các khía cạnh tài nguyên du lịch, xu hướng phát triển du lịch, sự ủng hộ của người dân địa phương và sự quan tâm của chính quyền địa phương. Tuy nhiên, yếu tố xu hướng phát triển thị trường du lịch nông nghiệp được đánh giá ở mức điểm thấp hơn. Điều này cũng xuất phát từ việc phát triển du lịch nông nghiệp tại các huyện, thị xã phía Bắc tỉnh Bình Định cũng mới bắt đầu nhận được sự quan tâm trong vài năm gần đây, số lượt khách tham quan các huyện, thị xã phía Bắc tỉnh Bình Định còn ít so với tiềm năng du lịch của địa bàn

Nitrite metabolism of several bacterial strains isolated from abattoir and swine wastewater after biogas treatment

In nitrogen treatment with biological methods, nitrite metabolism is an intermediate process that facilitates other processes involving different bacteria strains. In this study, we isolated two nitrite-oxidising bacteria strains from abattoir wastewater and wastewater from biogas tanks of an industrial pig farm in Ha Tinh province. The bacteria strains grow, develop, and metabolise nitrite at pH 6–8 and 30–37 °C. The samples with the nitrite concentration up to 750 mg·L–1 were oxidised within four days of incubation, and the nitrite metabolism rate was proportional to the concentration of nitrite tested. Under severe conditions (salinity up to 3% NaCl, a low dissolved oxygen level of 0.1 mg·L–1), the two isolated bacterial strains exhibited their effective growth and nitrite metabolism capacity. The results enrich the database of nitrite-oxidising bacteria and are prospective in wastewater treatment

Investigation of anti-inflammatory lignans from the leaves of Symplocos sumuntia Buch-Ham ex D Don (Symplocaceae)

Purpose: To investigate the anti-inflammatory activity of Symplocos sumuntia Buch.-Ham. ex D. Don and identify the main secondary metabolites responsible for this effect.Methods: The in vitro anti-inflammatory activity of the plant extract and isolated compounds was determined in terms of the ability to inhibit the production of nitric oxide (NO), and expressions of iNOS and COX-2 proteins in RAW264.7 cells stimulated by lipopolysaccharide (LPS). Compounds were isolated and identified by spectroscopic methods.Results: The methanol extract of S. sumuntia leaves showed strong inhibitory effects on nitric oxide (NO) production and expression of iNOS and COX-2 in LPS-induced RAW264.7 cells. A phytochemical assay-guided fractionation of the methanol extract of S. sumuntia leaves led to the isolation of four lignans which are arctigenin (1), matairesinol (2), monomethylpinoresinol (3) and pinoresinol (4). These compounds were identified for the first time from S. sumuntia. All four compounds inhibited the production of nitric oxide (NO), with arctigenin showing the most potent activity with half-maximal inhibitory concentration (IC50) value of 4.08 μM.Conclusion: S. sumuntia is a promising source of anti-inflammatory agents, which may clarify to the therapeutic use of this plant in Vietamese traditional medicine.Keywords: Symplocos sumuntia, Symplocos caudata, Lignan, Arctigenin, Anti-inflammator

Vietnam’s Scientific Publications in the Period of COVID-19

The COVID-19 pandemic is considered a global disaster that affects all areas of the world; however, it is also seen as a motivation for domestic and foreign scientists to focus on researching solutions to reduce its damage. This article aims to explore the correlation of scientific publications of countries in Southeast Asia, among research fields in Vietnam and among topics published by Vietnamese educational institutions in the context of a pandemic. 1392 Southeast Asian countries’ publications related to COVID-19 were referenced from the Scopus database, including 123 articles from Vietnam (up to August 27th, 2020). Statistics show that Vietnam ranks fifth in the number of scientific publications with research cooperation of researchers from 20 different countries. Regarding the research fields of Vietnam, medicine is the main research topic, social science ranks third following environmental science. In the field of social science, articles focus on four key topics: epidemic prevention, reduction of pandemic effects on life and socioeconomics, factors related to online learning of students, healthcare for the elderly. From the analysis results, the authors recommend that researchers should pay attention to other topics in the social sciences that have not been published, such as psychological effects of infected or suspected nCovi, the impact of COVID-19 to disadvantaged groups in society..

Tensile, electrical properties and morphology of polyethylene/modified fly ash composites using ultraflow

This paper presents relative melt viscosity, tensile, electrical properties and morphology of high density polyethylene (HDPE)/organo-modified fly ash (MFA) and HDPE/MFA/ultraflow (UTF) composites which were prepared by melt mixing method. Relative melt viscosity of HDPE was decreased with adding MFA and UTF into HDPE. Tensile properties (tensile strength, elongation at break and Young’s modulus) of HDPE/MFA/UTF composites were increased with rising UTF content to 5 wt.% and thereafter, they were dropped with the UTF content more than 5 wt.%. Electric properties (dielectric constant, dielectric loss and volume resistivity) of the HDPE/MFA and HDPE/MFA/UTF composites were investigated. The obtained parameters showed that the HDPE/MFA composites have electric insulation higher than HDPE/MFA/UTF composites. Morphology of the composite materials with and without using UTF was also studied by Field Emission Scanning Electron Microscopy (FESEM) images. The results indicated that the MFA was dispersed more regularly and less agglomerated in HDPE matrix with adding UTF into the HDPE/MFA composites

Simultaneously induced mutations in eIF4E genes by CRISPR/Cas9 enhance PVY resistance in tobacco

Tobacco is an important commercial crop and a rich source of alkaloids for pharmaceutical and agricultural applications. However, its yield can be reduced by up to 70% due to virus infections, especially by a potyvirus Potato virus Y (PVY). The replication of PVY relies on host factors, and eukaryotic translation initiation factor 4Es (eIF4Es) have already been identified as recessive resistance genes against potyviruses in many plant species. To investigate the molecular basis of PVY resistance in the widely cultivated allotetraploid tobacco variety K326, we developed a dual guide RNA CRISPR/Cas9 system for combinatorial gene editing of two clades, eIF4E1 (eIF4E1-S and eIF4E1-T) and eIF4E2 (eIF4E2-S and eIF4E2-T) in the eIF4E gene family comprising six members in tobacco. We screened for CRISPR/Cas9-induced mutations by heteroduplex analysis and Sanger sequencing, and monitored PVY(O) accumulation in virus challenged regenerated plants by DAS-ELISA both in T0 and T1 generations. We found that all T0 lines carrying targeted mutations in the eIF4E1-S gene displayed enhanced resistance to PVY(O) confirming previous reports. More importantly, our combinatorial approach revealed that eIF4E1-S is necessary but not sufficient for complete PVY resistance. Only the quadruple mutants harboring loss-of-function mutations in eIF4E1-S, eIF4E1-T, eIF4E2-S and eIF4E2-T showed heritable high-level resistance to PVY(O) in tobacco. Our work highlights the importance of understanding host factor redundancy in virus replication and provides a roadmap to generate virus resistance by combinatorial CRISPR/Cas9-mediated editing in non-model crop plants with complex genomes

Investigation of salt-tolerant rhizosphere bacteria from seawater-intruding paddy rice field in Vietnam

Salt‐tolerant plant growth‐promoting rhizobacteria (ST‐PGPR) are known as potential tools to improve rice salinity tolerance. In this study, we aimed to investigate the plant growth‐promoting rhizobacteria community richness of the paddy rice fields in Soc Trang and Ben Tre Provinces where were seriously affected by sea level rise. The salinity in the sampling sites ranged from 0.14‰ to 2.17‰ in November 2018, the rainy season. The microbial abundance of samples was evaluated by spreading the samples in tryptic soy agar (TSA) medium supplemented with various concentrations of NaCl. With the increase of salt concentration up to 10% NaCl, a total number of bacteria decreased for all the samples, ranging from 106 to 104 CFU/g, and bacterial colonies were not observed at 30% NaCl. Among a total of 48 salt-resisting bacteria isolated from the rice paddy field mud surrounding the rice root, 22 isolates were able to produce indole-3-acetic acid (IAA: phytohormone for the plant growth). Seventeen out of 48 isolates were able to grow in the medium without nitrogen or phosphor sources. Six isolates having high IAA producing activity, nitrogen fixation and phosphate solubilization were belonged to Bacillus (DT6, LT16, and LHT8), Halobacillus (DT8), Aeromonas (LHT1), and Klebsiella (LHT7) genera. All the sequences of the strains DT6, DT8, LT16, LHT1, LHT7, and LHT8 were registered in the GeneBank with the accession numbers MK335670, MK335671, MK335672, MK335673, MK335674, and MK335675, respectively.

Characterization of Ag-Doped p-Type SnO Thin Films Prepared by DC Magnetron Sputtering

Crystalline structure and optoelectrical properties of silver-doped tin monoxide thin films with different dopant concentrations prepared by DC magnetron sputtering are investigated. The X-ray diffraction patterns reveal that the tetragonal SnO phase exhibits preferred orientations along (101) and (110) planes. Our results indicate that replacing Sn2+ in the SnO lattice with Ag+ ions produces smaller-sized crystallites, which may lead to enhanced carrier scattering at grain boundaries. This causes a deterioration in the carrier mobility, even though the carrier concentration improves by two orders of magnitude due to doping. In addition, the Ag-doped SnO thin films show a p-type semiconductor behavior, with a direct optical gap and decreasing transmittance with increasing Ag dopant concentration

Evaluation of Xpert MTB/RIF and MODS assay for the diagnosis of pediatric tuberculosis

BACKGROUND: Tuberculosis (TB) in children is rarely confirmed due to the lack of effective diagnostic tools; only 10 to 15% of pediatric TB is smear positive due to paucibacillary samples and the difficulty of obtaining high-quality specimens from children. We evaluate here the accuracy of Xpert MTB/RIF in comparison with the Micoroscopic observation drug susceptibility (MODS) assay for diagnosis of TB in children using samples stored during a previously reported evaluation of the MODS assay. METHODS: Ninety-six eligible children presenting with suspected TB were recruited consecutively at Pham Ngoc Thach Hospital in Ho Chi Minh City Viet Nam between May to December 2008 and tested by Ziehl-Neelsen smear, MODS and Mycobacterial growth Indicator (MGIT, Becton Dickinson) culture. All samples sent by the treating clinician for testing were included in the analysis. An aliquot of processed sample deposit was stored at −20°C and tested in the present study by Xpert MTB/RIF test. 183 samples from 73 children were available for analysis by Xpert. Accuracy measures of MODS and Xpert were summarized. RESULTS: The sensitivity (%) in detecting children with a clinical diagnosis of TB for smear, MODS and Xpert were 37.9 [95% CI 25.5; 51.6], 51.7 [38.2; 65.0] and 50.0 [36.6; 63.4], respectively (per patient analysis). Xpert was significantly more sensitive than smear (P=0.046). Testing of additional samples did not increase case detection for MODS while testing of a second sputum sample by Xpert detected only two additional cases. The positive and negative predictive values (%) of Xpert were 100.0 [88.0; 100.0] and 34.1 [20.5; 49.9], respectively, while those of MODS were 96.8 [83.3; 99.9] and 33.3 [19.6; 49.5]. CONCLUSION: MODS culture and Xpert MTB/RIF test have similar sensitivities for the detection of pediatric TB. Xpert MTB RIF is able to detect tuberculosis and rifampicin resistance within two hours. MODS allows isolation of cultures for further drug susceptibility testing but requires approximately one week to become positive. Testing of multiple samples by xpert detected only two additional cases and the benefits must be considered against costs in each setting. Further research is required to evaluate the optimal integration of Xpert into pediatric testing algorithms

Deficient Mismatch Repair Proteins in Gastric Mixed Neuroendocrine Non-Neuroendocrine Neoplasm: A Rare Case Report

Mixed neuroendocrine non-neuroendocrine neoplasm (MiNEN) is a rare type of gastric carcinoma with controversial diagnosis and treatment. Recent data implies that deficiency mismatch repair proteins inducing microsatellite instability are considered one of the potential drivers of this disease. Hence, we report a stomach MiNEN with MMR protein loss. An admitted 60-year-old woman complained of epigastric pain. The pathological analysis of the gastro-endoscopic biopsy specimen revealed gastric adenocarcinoma. The radiological staging was cT3N1M0; therefore, she received D2 distal gastrectomy. Suspecting neuroendocrine component admix with adenocarcinoma part on the resected specimen microscopy, applying biomarkers including AE 1/3, synaptophysin, and chromogranin A to confirm the diagnosis of MiNEN. The neuroendocrine part was classified as neuroendocrine tumor grade 2 with Ki 67 at 16.5%. To further understand the molecular characterization of this disease, we evaluated mismatch protein expression by staining MLH1, MSH2, MSH6, and PMS2 antibodies. Interestingly, both components lost MLH1 and PMS2 proteins. Her radical surgery followed oxaliplatin/capecitabine adjuvant chemotherapy. The patient is still well after eight cycles of chemotherapy. dMMR gastric MiNENs and dMMR gastric cancer share many clinical and genetic characteristics. Further studies are necessary to survey the role of dMMR in the prognosis and treatment of this entity