Extraction protocol for miRNA.

(PDF)</p

Mean, standard deviation (SD) and standard error of the mean (SE) from clinical parameters (blood count, biochemical and urinalysis) of PKD group.

Where Hematocrit (HCT), Red Blood Cell (RBC), Hemoglobin (HGB), Mean corpuscular volume (MCV), Mean corpuscular hemoglobin concentration (MCHC), White blood cell (WBC), Blood Urea Nitrogen (BUN), Phosphorus (P), Potassium (K), Symmetric dimethilarginine (SDMA), Urine Protein/Creatinine Ratio (UPCR), Gamma-Glutamyl Transpeptidase (GGT). (PDF)</p

Cycle threshold values (mean ± SEM) for microRNA derived from feline plasma and serum samples of the PKD group.

Cycle threshold values (mean ± SEM) for microRNA derived from feline plasma and serum samples of the PKD group.</p

microRNAs, stem-loop RT primers, mature microRNA sequences, and physiologic or pathologic correlation [8].

microRNAs, stem-loop RT primers, mature microRNA sequences, and physiologic or pathologic correlation [8].</p

Fold change of serum microRNAs (miR-20a, miR-192, miR-365, miR-16-5p, miR-15b-5p) from domestic cat.

RT-rtPCR assays. Normalized with cel-miR-39-3p. miR-20a is upregulated in PKD group *(p p values were p > 0.05.</p

Quantification protocol for miRNA.

(PDF)</p

Fold change of plasma microRNAs (miR-20a, miR-192, miR-365, miR-16-5p, miR-15b5p) from domestic cat.

RT-rtPCR assays. Normalized with cel-miR-39-3p (p > 0.05).</p

Real time PCR protocol for miRNA.

(PDF)</p

Clinical parameters (blood count, biochemical and urinalysis) of individual samples.

Hematocrit (HCT), Red Blood Cell (RBC), Hemoglobin (HGB), Mean corpuscular volume (MCV), Mean corpuscular hemoglobin concentration (MCHC), White blood cell (WBC), Blood Urea Nitrogen (BUN), Phosphorus (P), Potassium (K), Symmetric dimethilarginine (SDMA), Urine Protein/Creatinine Ratio (UPCR), Gamma-Glutamyl Transpeptidase (GGT). (PDF)</p

Reverse transcription protocol for miRNA.

(PDF)</p