21 research outputs found
Filarial nematodes used to assess the analytical specificity of the qPCR assay.
<p>Filarial nematodes used to assess the analytical specificity of the qPCR assay.</p
Detection limit of the conventional PCR assay determined by 10-fold serial dilution of genomic DNA of microfilariae and adult of <i>Onchocerca lupi</i>.
<p>Lanes 1–4, from 3.6 ×10<sup>1</sup> pg/2μl to 3.6 ×10<sup>−3</sup> pg/2μl of <i>O</i>. <i>lupi</i> mfs DNA (i.e., from 1 to 1×10<sup>−4</sup> mfs); Lanes 5–15, from 8 ×10<sup>1</sup> ng/2μl to 8 x 10<sup>−3</sup> fg/2μl of <i>O</i>. <i>lupi</i> adult DNA; Line 16, no-DNA control; M, 100 bp DNA marker.</p
A real-time PCR tool for the surveillance of zoonotic <i>Onchocerca lupi</i> in dogs, cats and potential vectors - Fig 2
<p>Standard curves generated from serial dilutions of (A) genomic DNA from adult (from 8 × 10<sup>4</sup> to 8 × 10<sup>−3</sup> fg/2μl of reaction) and microfilariae (B) (from 3.6 ×10<sup>−1</sup> ng/2μl to 3.6 ×10<sup>1</sup> fg/2μl of reaction) of <i>Onchocerca lupi</i>. Each point was tested in triplicate. Slope, efficacy and <i>R</i><sup>2</sup> are reported on the bottom.</p
GenBank accession numbers (AN) of mitochondrial cytochrome <i>c</i> oxidase subunit 1 sequences of filarial nematodes used for primers and TaqMan-probe design.
<p>GenBank accession numbers (AN) of mitochondrial cytochrome <i>c</i> oxidase subunit 1 sequences of filarial nematodes used for primers and TaqMan-probe design.</p
Skin samples tested for <i>Onchocerca lupi</i> by qPCR, divided (Groups 1–5) according to the parasitic load (mfs) microscopically detected.
<p>The mean, minimum, maximum and standard deviation (sd) values of the threshold cycle (Cq), parasite load (Starting Quantity (SQ) value, expressed as ng/μl of DNA for reaction) and microfilariae concentration, assessed by qPCR is reported.</p
Assessment of the specificity of qPCR assay in the detection of <i>Onchocerca lupi</i> DNA.
<p>The amplification plot is represented by the fluorescent signal accordingly to relative fluorescence units (RFU) and threshold cycle.</p
Pathogens screened in this study by conventional (c) and quantitative (q) PCR, with target genes, primers, probes nucleotide sequences and fragment length.
Pathogens screened in this study by conventional (c) and quantitative (q) PCR, with target genes, primers, probes nucleotide sequences and fragment length.</p
Map of the Jemaa-El-Fna square, Marrakech, Morocco.
Blue circle represents Marrakech municipality; Yellow circle represents site where snakes used by charmers are displayed, and site where reptiles are sold by vendors is represented by a red circle. Map prepared using QGIS software—Buenos Aires version (link of the XYZ tile: https://tile.openstreetmap.org).</p
Species of reptiles (scientific and common names) sampled along with numbers and type of owners.
Species of reptiles (scientific and common names) sampled along with numbers and type of owners.</p
Vector-Borne pathogens and oral-fecal pathogens detected in reptiles by blood smear or molecular identification.
Vector-Borne pathogens and oral-fecal pathogens detected in reptiles by blood smear or molecular identification.</p