Two canines derived from SCNT.

<p>(A) Canine derived using RIA and (B) ECLI methods.</p

Canine cloned using <i>in vivo</i> matured oocytes as predicted by RIA and ECLI methods.

<p>Canine cloned using <i>in vivo</i> matured oocytes as predicted by RIA and ECLI methods.</p

Classification of canine oocytes.

<p>Oocytes were categorized as immature, mature, and aged.</p

Percentage of oocyte donors at each stage detected by (A) RIA or (B) ECLI methods using P4 concentrations of 4–8 ng/mL or (C) 6–15 ng/mL as ovulation standards.

<p>Pre: preovulation, IM, immature, M, mature and A, aging. Thirty dogs were used for analysis in each group.</p

Comparison of progesterone concentrations (ng/mL) based on RIA and ECLI methods.

<p>(a'–j') Samples were separated based on the RIA data. Colocalization of RIA and ECLI methods were evaluated using the Pearson correlation coefficient. B) Average of RIA and ECLI methods. Data are presented as mean ± SD. The asterisk denotes significant differences (P<0.05).</p

El Diario de Pontevedra : periódico liberal: Ano XXV Número 7145 - 1908 febreiro 12

<p><b>Production of AD transgenic cloned pigs and identification of AD transgenes (A), hAPP, hTau, and hPS1 in cloned pigs: #1, 2 (alive, B), and 3 (stillborn).</b> Expression of APP-Tau and Tau-PS1 in the tissues of AD transgenic pig, determined by real-time RT-PCR (C) and expression of AD-related genes in brain of TG pigs (D). hAPP, human amyloid precursor protein; hTau, human tau protein; hPS1: human presenilin 1. The experiment was repeated three or four independent times.</p

Bio-marker analysis of Tg pigs.

<p>(A) Quantification of Aβ1–40, Aβ1–42, and total Tau in the cortex of control (blank box) and Tg brains (black bar) using ELISA. All three gene expression was elevated in the TG brain. (B) Expression of APP in the cortex region of JNUPIGs confirmed by immunohistochemistry stained with anti-Aβ specific antibody. (C) Astrocyte activation, monitored by GFAP level, in control and Tg brain. GFAP level was highly elevated in TG brain. Images, taken at 400× magnification, are representative of at least four sections per animal. Data were analyzed by independent samples t-test in SPSS (*: p < 0.05, **: p < 0.01). The experiment was repeated three independent times.</p

Full-term development of AD transgenic piglets.

<p>Full-term development of AD transgenic piglets.</p

Predicted multiple insertion sites in chromosome X of transgenic pigs.

<p>The insertion site was determined by NGS analysis of gDNA of a transgenic pig. The transgene was inserted into the region between exons 9 and 10 of <i>BEND2</i>.</p

Preparation of AD transgenic Jeju black pig ear fibroblasts (JB-PEF^AD).

<p>Morphology (A), integration (B), and mRNA expression (C) of AD multi-cistronic vector in JB-PEF^AD cells. Karyotype (D) of JB-PEF^AD cell lines, revealing normal chromosome number (2n = 38). AD multi-cistronic vector: hAPP, human amyloid precursor protein; hTau, human tau protein; PS1, presenilin 1. Scale bars = 20 μm.</p