Schematic diagram of the metabolic pathway and relative levels of the major compounds detected in <i>C. pruinosa</i> extracted with 70% MeOH and 100% <i>n-</i>hexane plus D₂O.

<p>This was modified from pathways presented in KEGG database (<a href="http://www.genome.jp/kegg/" target="_blank">http://www.genome.jp/kegg/</a>). ANOVA was performed to assess the statistical significance of differences between samples (p<0.05). Data are mean values with error bars representing standard deviation values. Different letters in bars represent the difference of statistical significance of metabolites levels. The superscript numbers in compounds represent analysis methods. 1: NMR analysis of D₂O extracts, 2: GC-MS analysis of 70% methanol extracts, 3: GC-MS analysis of n-hexane extracts.</p

Metabolites identified in 70% methanol extractsof <i>C. bassiana</i> fruiting bodies using GC-MS.

*<p>No annotation in enrichment analysis.</p

The list of metabolites identified by ¹H NMR and GC-MS analysis.

<p>The list of metabolites identified by ¹H NMR and GC-MS analysis.</p

Metabolites identified in 100% <i>n</i>-hexane extracts of <i>C. bassiana</i> fruiting bodies using GC-MS.

*<p>No annotation in enrichment analysis.</p

Assignment of ¹H NMR spectral peaks for <i>C. pruinosa</i> mycelia using D₂O extracts.

<p>Assignment of ¹H NMR spectral peaks for <i>C. pruinosa</i> mycelia using D₂O extracts.</p

PLS-DA-derived score plots from 70% methanol (A), and 100% <i>n</i>-hexane (B) extracts of cultivated <i>C</i>. <i>bassiana</i> at various development stages.

<p>stage 1; □, stage 2; •, stage 3; △, stage 4; ♦.</p

PCA score plots of <i>C. pruinosa</i> mycelia cultivated under various conditions.

<p>(A) D₂O extracts characterized using NMR analysis. (B) 70% methanol extracts and (C) 100% <i>n</i>-hexane extracts characterized using GC-MS analysis. PCA, Principal component analysis.</p

Chromatographic data of the identified compounds from the 100% <i>n</i>-hexane extract of <i>C. pruinosa</i> mycelia analyzed using GC-MS.

<p>Chromatographic data of the identified compounds from the 100% <i>n</i>-hexane extract of <i>C. pruinosa</i> mycelia analyzed using GC-MS.</p

Morphological characteristics of <i>C. pruinosa</i> mycelia cultivated under various conditions.

<p>Left photo in each condition: front of plate. Right photo in each condition: back of plate. (A) S+L condition; (B) S+D condition; (C) L+D condition; (D) SL+D condition; (E) SF+D condition, (F) N+D condition.</p

Antioxidant ability and total phenolic content of the 70% methanol extracts of <i>C. pruinosa</i> mycelium grown in different cultivation conditions.

<p>GAE: gallic acid equivalents.</p