Isolation and Enrichment of Bacteriophages by Membrane Filtration Immobilization Technique

The method described here enables rapid bacteriophage isolation and enrichment of host-specific bacteriophages from an environmental sample. This is achieved by using a simple 0.45-μm Millipore membrane where a specific host is immobilized on the membrane and a sample suspected of containing bacteriophages is exposed to the immobilized cells with the help of a membrane filtration unit. This filtration step facilitates host-specific interaction of bacteriophages with the host and maximization of this interaction using a classic membrane filtration method. Under the effect of vacuum from a vacuum pump, a filter assembly provides a chance for every bacteriophage in the sample to interact with the specific host on the membrane filter. Our technique allows retaining specific bacteriophages on the membrane along with its host cells via adsorption; these adsorbed bacteriophages (along with their hosts) on a filter disc are then enriched in regular nutritive broth, tryptone soya broth (TSB), by incubation. With help of a plaque assay method, host-specific phages of various bacterial species can be isolated, segregated, and enriched. C � 2018 by John Wiley & Sons, Inc

Pressure drop behaviour for high liquid-gas ratios in packed column

221-223<span style="font-size:11.0pt;line-height:115%; font-family:" calibri","sans-serif";mso-ascii-theme-font:minor-latin;mso-fareast-font-family:="" "times="" new="" roman";mso-fareast-theme-font:minor-fareast;mso-hansi-theme-font:="" minor-latin;mso-bidi-font-family:arial;mso-ansi-language:en-us;mso-fareast-language:="" en-us;mso-bidi-language:ar-sa"="">Pressure drop behaviour in packed column with air-water system has been studied experimentally to investigate the performance of packing in the range where the Sherwood parameter... </span

Biointerface Properties of Core-Shell Poly(vinyl alcohol)-hyaluronic Acid Microgels Based on Chemoselective Chemistry

Chemoselective chemistry is one of the main synthetic strategies for the design of bioactive constructs. In this contribution we report on the fabrication of core-shell microgel particles, obtained by "click chemistry" and "inverse emulsion droplets" techniques. Azido and alkyne derivatives of poly(vinyl alcohol) (PVA) in a 1:2 mol ratio of functional groups, respectively, were crosslinked by click chemistry method. The microgel particles were spherical in shape with an average diameter of about 2 μm and with a narrow size distribution. Residual unreacted alkyne groups present on the particle surface were "clicked" with an azido-grafted hyaluronic acid. These microgel particles with a PVA core and a hyaluronic acid shell were tested for bioorthogonality, that is, for the absence of cytotoxicity in the presence of unreacted clickable functionalities and demonstrated a remarkable ability to target adenocarcinoma colon cells (HT- 29) as well as to release locally the antitumor drug, doxorubicin. Internalization process was studied in connection with the presence of hyaluronic acid on the microgel particles surface. In this paper we introduce a concept device based on chemoselective chemistry, which may contribute to the design of micro- and nanoplatforms having controlled and multifunctional structures

Membrane filtration immobilization technique—a simple and novel method for primary isolation and enrichment of bacteriophages

To develop a method for the isolation and enrichment of bacteriophages selectively against specific bacteria coupled with a membrane filtration technique. Methods and Results: Rapid isolation and concentration of host-specific bacteriophages was achieved by exposure of the sample suspected to contain bacteriophages to a specific host immobilized on a 0�45 lm membrane in a membrane filtration unit. The principle behind this method is the exploitation of host-specific interaction of bacteriophages with their host and maximizing this interaction using a classic membrane filtration method. This provides a chance for each bacteriophage in the sample to interact with the specific host on the membrane filter fitted with a vacuum pump. Specific bacteriophages of the host are retained on the membrane along with its host cells due to the effect of adsorption and these adsorbed bacteriophages (along with their hosts) on the filter disc are then amplified and enriched in regular nutritive broth tryptose soya broth by incubation. With the help of the plaque assay method, host-specific phages of various bacterial species were isolated, segregated and enriched. Conclusions: The phage concentration method coupled with membrane filtration immobilization of host bacteria was able to isolate and enrich the host-specific bacteriophages by several fold using a lower quantity of an environmental water sample, or other phage suspensions. Enrichment of phages from single plaques was also achieved. Significance and Impact of the Study: The isolation and detection of hostspecific bacteriophages from a low density bacteriophage water sample in a single step by the use of a simple and basic microbiological technique can be achieved. Enrichment of phages from low phage titre suspensions is also achieved very effectivel

You have full text access to this contentMembrane filtration immobilization technique—a simple and novel method for primary isolation and enrichment of bacteriophages

Aim: To develop a method for the isolation and enrichment of bacteriophages selectively against specific bacteria coupled with a membrane filtration technique. Methods and Results: Rapid isolation and concentration of host-specific bacteriophages was achieved by exposure of the sample suspected to contain bacteriophages to a specific host immobilized on a 0�45 lm membrane in a membrane filtration unit. The principle behind this method is the exploitation of host-specific interaction of bacteriophages with their host and maximizing this interaction using a classic membrane filtration method. This provides a chance for each bacteriophage in the sample to interact with the specific host on the membrane filter fitted with a vacuum pump. Specific bacteriophages of the host are retained on the membrane along with its host cells due to the effect of adsorption and these adsorbed bacteriophages (along with their hosts) on the filter disc are then amplified and enriched in regular nutritive broth tryptose soya broth by incubation. With the help of the plaque assay method, host-specific phages of various bacterial species were isolated, segregated and enriched. Conclusions: The phage concentration method coupled with membrane filtration immobilization of host bacteria was able to isolate and enrich the host-specific bacteriophages by several fold using a lower quantity of an environmental water sample, or other phage suspensions. Enrichment of phages from single plaques was also achieved. Significance and Impact of the Study: The isolation and detection of hostspecific bacteriophages from a low density bacteriophage water sample in a single step by the use of a simple and basic microbiological technique can be achieved. Enrichment of phages from low phage titre suspensions is also achieved very effectively