LYVE1 Marks the Divergence of Yolk Sac Definitive Hemogenic Endothelium from the Primitive Erythroid Lineage.

The contribution of the different waves and sites of developmental hematopoiesis to fetal and adult blood production remains unclear. Here, we identify lymphatic vessel endothelial hyaluronan receptor-1 (LYVE1) as a marker of yolk sac (YS) endothelium and definitive hematopoietic stem and progenitor cells (HSPCs). Endothelium in mid-gestation YS and vitelline vessels, but not the dorsal aorta and placenta, were labeled by Lyve1-Cre. Most YS HSPCs and erythro-myeloid progenitors were Lyve1-Cre lineage traced, but primitive erythroid cells were not, suggesting that they represent distinct lineages. Fetal liver (FL) and adult HSPCs showed 35%-40% Lyve1-Cre marking. Analysis of circulation-deficient Ncx1-/- concepti identified the YS as a major source of Lyve1-Cre labeled HSPCs. FL proerythroblast marking was extensive at embryonic day (E) 11.5-13.5, but decreased to hematopoietic stem cell (HSC) levels by E16.5, suggesting that HSCs from multiple sources became responsible for erythropoiesis. Lyve1-Cre thus marks the divergence between YS primitive and definitive hematopoiesis and provides a tool for targeting YS definitive hematopoiesis and FL colonization

LYVE1 as a Marker for Definitive Hematopoietic Stem Cell Ontogeny

The question of the developmental origins of hematopoietic stem cells (HSCs) is an actively investigated topic in the hematopoiesis field. It is an important subject to address because of its potential impact on identifying and treating the root cause of hematopoietic disorders and diseases. It had been found that LYVE1, which is most commonly known as a lymphatic vessel marker, identifies a population of blood vessel endothelial cells in the embryo and hematopoietic cells in the adult mouse (Pham et al. 2010). Over the course of my dissertation research, we sought to determine the contribution of LYVE1-derived cells to the adult (definitive) HSC population. We utilized a lineage tracing system and identified that LYVE1 marks definitive hemogenic endothelium— a subset of endothelial cells that gives rise to hematopoietic cells early in development— in the yolk sac and the AGM. We found that these LYVE1-derived pre-HSCs take longer to mature than their non-LYVE1-derived counterparts and need to receive maturation signals from the intra-embryonic environment in order to become fully functional. These LYVE1-derived cells persist to adulthood and contribute to approximately a third of the adult murine HSC pool. During the course of these studies, we also found the importance of utilizing different reporter systems in order to validate the phenotypes identified using Cre-Lox recombination systems. Overall, the findings in these studies indicate that there are multiple pools of HSCs (Lyve1-derived and not) and that each pool has a unique origin. The heterogeneity of the HSC pool should be considered when differentiating these cells from iPSCs for both research and clinical translational purposes. It should also be something that is considered during the treatment of different hematopoietic disorders, as there may be a healthy and diseased pool of HSCs that corresponds with their developmental origins

LYVE1 as a Marker for Definitive Hematopoietic Stem Cell Ontogeny

The question of the developmental origins of hematopoietic stem cells (HSCs) is an actively investigated topic in the hematopoiesis field. It is an important subject to address because of its potential impact on identifying and treating the root cause of hematopoietic disorders and diseases. It had been found that LYVE1, which is most commonly known as a lymphatic vessel marker, identifies a population of blood vessel endothelial cells in the embryo and hematopoietic cells in the adult mouse (Pham et al. 2010). Over the course of my dissertation research, we sought to determine the contribution of LYVE1-derived cells to the adult (definitive) HSC population. We utilized a lineage tracing system and identified that LYVE1 marks definitive hemogenic endothelium— a subset of endothelial cells that gives rise to hematopoietic cells early in development— in the yolk sac and the AGM. We found that these LYVE1-derived pre-HSCs take longer to mature than their non-LYVE1-derived counterparts and need to receive maturation signals from the intra-embryonic environment in order to become fully functional. These LYVE1-derived cells persist to adulthood and contribute to approximately a third of the adult murine HSC pool. During the course of these studies, we also found the importance of utilizing different reporter systems in order to validate the phenotypes identified using Cre-Lox recombination systems. Overall, the findings in these studies indicate that there are multiple pools of HSCs (Lyve1-derived and not) and that each pool has a unique origin. The heterogeneity of the HSC pool should be considered when differentiating these cells from iPSCs for both research and clinical translational purposes. It should also be something that is considered during the treatment of different hematopoietic disorders, as there may be a healthy and diseased pool of HSCs that corresponds with their developmental origins

Absence of CD11a Expression Identifies Embryonic Hematopoietic Stem Cell Precursors via Competitive Neonatal Transplantation Assay.

Hematopoietic stem cells (HSCs) are defined by their self-renewal, multipotency, and bone marrow (BM) engraftment abilities. How HSCs emerge during embryonic development remains unclear, but are thought to arise from hemogenic endothelium through an intermediate precursor called "pre-HSCs." Pre-HSCs have self-renewal and multipotent activity, but lack BM engraftability. They can be identified functionally by transplantation into neonatal recipients, or by in vitro co-culture with cytokines and stroma followed by transplantation into adult recipients. While pre-HSCs express markers such as Kit and CD144, a precise surface marker identity for pre-HSCs has remained elusive due to the fluctuating expression of common HSC markers during embryonic development. We have previously determined that the lack of CD11a expression distinguishes HSCs in adults as well as multipotent progenitors in the embryo. Here, we use a neonatal transplantation assay to identify pre-HSC populations in the mouse embryo. We establish CD11a as a critical marker for the identification and enrichment of pre-HSCs in day 10.5 and 11.5 mouse embryos. Our proposed pre-HSC population, termed "11a- eKLS" (CD11a- Ter119- CD43+ Kit+ Sca1+ CD144+), contains all in vivo long-term engrafting embryonic progenitors. This population also displays a cell-cycle status expected of embryonic HSC precursors. Furthermore, we identify the neonatal liver as the likely source of signals that can mature pre-HSCs into BM-engraftable HSCs

MAC2 is a long‐lasting marker of peripheral cell infiltrates into the mouse CNS after bone marrow transplantation and coronavirus infection

Microglia are the primary resident myeloid cells of the brain responsible for maintaining homeostasis and protecting the central nervous system (CNS) from damage and infection. Monocytes and monocyte-derived macrophages arising from the periphery have also been implicated in CNS pathologies, however, distinguishing between different myeloid cell populations in the CNS has been difficult. Here, we set out to develop a reliable histological marker that can assess distinct myeloid cell heterogeneity and functional contributions, particularly in the context of disease and/or neuroinflammation. scRNAseq from brains of mice infected with the neurotropic JHM strain of the mouse hepatitis virus (JHMV), a mouse coronavirus, revealed that Lgals3 is highly upregulated in monocyte and macrophage populations, but not in microglia. Subsequent immunostaining for galectin-3 (encoded by Lgals3), also referred to as MAC2, highlighted the high expression levels of MAC2 protein in infiltrating myeloid cells in JHMV-infected and bone marrow (BM) chimeric mice, in stark contrast to microglia, which expressed little to no staining in these models. Expression of MAC2 was found even 6-10 months following BM-derived cell infiltration into the CNS. We also demonstrate that MAC2 is not a specific label for plaque-associated microglia in the 5xFAD mouse model, but only appears in a distinct subset of these cells in the presence of JHMV infection or during aging. Our data suggest that MAC2 can serve as a reliable and long-lasting histological marker for monocyte/macrophages in the brain, identifying an accessible approach to distinguishing resident microglia from infiltrating cells in the CNS under certain conditions

Effects of long-term and brain-wide colonization of peripheral bone marrow-derived myeloid cells in the CNS.

BackgroundMicroglia, the primary resident myeloid cells of the brain, play critical roles in immune defense by maintaining tissue homeostasis and responding to injury or disease. However, microglial activation and dysfunction has been implicated in a number of central nervous system (CNS) disorders, thus developing tools to manipulate and replace these myeloid cells in the CNS is of therapeutic interest.MethodsUsing whole body irradiation, bone marrow transplant, and colony-stimulating factor 1 receptor inhibition, we achieve long-term and brain-wide (~ 80%) engraftment and colonization of peripheral bone marrow-derived myeloid cells (i.e., monocytes) in the brain parenchyma and evaluated the long-term effects of their colonization in the CNS.ResultsHere, we identify a monocyte signature that includes an upregulation in Ccr1, Ms4a6b, Ms4a6c, Ms4a7, Apobec1, Lyz2, Mrc1, Tmem221, Tlr8, Lilrb4a, Msr1, Nnt, and Wdfy1 and a downregulation of Siglech, Slc2a5, and Ccl21a/b. We demonstrate that irradiation and long-term (~ 6 months) engraftment of the CNS by monocytes induces brain region-dependent alterations in transcription profiles, astrocytes, neuronal structures, including synaptic components, and cognition. Although our results show that microglial replacement with peripherally derived myeloid cells is feasible and that irradiation-induced changes can be reversed by the replacement of microglia with monocytes in the hippocampus, we also observe that brain-wide engraftment of peripheral myeloid cells (relying on irradiation) can result in cognitive and synaptic deficits.ConclusionsThese findings provide insight into better understanding the role and complexity of myeloid cells in the brain, including their regulation of other CNS cells and functional outcomes

Subventricular zone/white matter microglia reconstitute the empty adult microglial niche in a dynamic wave.

Microglia, the brain's resident myeloid cells, play central roles in brain defense, homeostasis, and disease. Using a prolonged colony-stimulating factor 1 receptor inhibitor (CSF1Ri) approach, we report an unprecedented level of microglial depletion and establish a model system that achieves an empty microglial niche in the adult brain. We identify a myeloid cell that migrates from the subventricular zone and associated white matter areas. Following CSF1Ri, these amoeboid cells migrate radially and tangentially in a dynamic wave filling the brain in a distinct pattern, to replace the microglial-depleted brain. These repopulating cells are enriched in disease-associated microglia genes and exhibit similar phenotypic and transcriptional profiles to white-matter-associated microglia. Our findings shed light on the overlapping and distinct functional complexity and diversity of myeloid cells of the CNS and provide new insight into repopulating microglia function and dynamics in the mouse brain