26 research outputs found
Phylogenetic Groups of Escherichia coli Strains from Patients with Urinary Tract Infection in Iran Based on the New Clermont Phylotyping Method
Objectives.In2013,ClermontclassifiedE.colistrainsintoeightphylogeneticgroupsusinganewquadruplexPCRmethod.Theaims ofthisstudyweretoidentifythephylogeneticgroupsofE.colibasedonthismethodandtoassesstheirantibioticresistancepatterns inBushehr,Iran.Methods.Inthiscross-sectionalstudy,140E.coliisolatesweresubjectedtophylogenetictypingbyaquadruplex PCR method. Antimicrobial susceptibility testing was performed by disk diffusion method. Results. Phylogenetic group B2 was mostpredominant(39.3%),followedbyunknown(27.1%),E(9.3%),CandcladeI(each6.4%),B1(5%),FandD(each2.9%),and A(0.7%).Themostcommonantibioticresistancewasrelatedtoamoxicillin(82.1%)andtheleasttomeropenem(0.7%).82.14%of isolatesweremultipledrugresistant(MDR).AntibioticresistancewasmainlydetectedingroupB2(50%).Conclusions.Ourfindings showedthehighprevalenceofMDRE.coliisolateswithdominanceofgroupB2.About25%ofE.coliisolatesbelongtothenewly describedphylogroupsC,E,F,andcladeI.Suchstudiesneedtobedonealsoinotherregionstoprovidegreaterunderstandingof theantibioticresistancepatternandtheprevalencesofdifferentphylogeneticgroups
Antibacterial activity of ethyl acetate and aqueous extracts of Mentha longifolia L. and hydroalcoholic extract of Zataria multiflora Boiss. plants against important human pathogens
AbstractObjectiveTo determine the potential antibacterial activity of ethyl acetate and aqueous extracts from Mentha longifolia L. (M. longifolia) and hydroalcoholic extract of Zataria multiflora Boiss. (Z. multiflora) against important human pathogens.MethodsPseudomonas aeruginosa, Shigella dysenteriae, Klebsiella pneumoniae (K. pneumonia), Enterobacter cloacae, Salmonella typhi, Proteus mirabilis, Serratia marcescens, Bacillus cereus, Staphylococcus saprophyticus and Staphylococcus aureus were kinds of pathogenic bacteria to determine the antibacterial effect of aqueous and ethyl acetate extracts of M. longifolia and hydroalcoholic extract of Z. multiflora using broth microdiluation method.ResultsThe lowest minimum inhibitory concentration and minimum bactericidal concentration values for K. pneumonia and Pseudomonas aeruginosa (1.25 and 2.5 mg/mL) were observed by the hydroalcoholic extract of Z. multiflora and the lowest minimum inhibitory concentration and minimum bactericidal concentration values for K. pneumonia and Serratia marcescens (2.5 and 5 mg/mL) were observed by the aqueous extracts of M. longifolia.ConclusionsIn conclusion, it seems that Z. multiflora and M. longifolia extracts could inhibit the growth of all of the mentioned bacteria
Investigation of caspase-1 activity and interleukin-1β production in murine macrophage cell lines infected with Leishmania major
AbstractObjectiveTo investigate the caspase-1 dependent inflammatory pathway activity and interleukin-1β (IL-1β) secretion in murine macrophage cell lines J774G8 infected with Leishmania major (L. major) using caspase-1 activity assay and ELISA.MethodsNovy-MacNeal-Nicolle biphasic medium was applied to produce promastigote form of L. major. Metacyclic promastigotes in the stationary phase were applied to infect macrophage. Caspase-1 activity and IL-1β secretion were assessed by the CPP32/caspase-1 fluorometric protease assay and ELISA IL-1β kits, respectively, with time intervals of 6, 18 and 30 h.ResultsOur study showed an increase in caspase-1 activity and IL-1β secretion in infected samples compared to non-infected macrophages. The highest increase in IL-1β production was observed after 6 h of infection.ConclusionsThese results arise that the activation of inflammasome pathway could be one of the innate immunity pathways against L. major
Retrieving ascarid and taeniid eggs from the biological remains of a Neolithic dog from the late 9th millennium BC in Western Iran
Bi-allelic ACBD6 variants lead to a neurodevelopmental syndrome with progressive and complex movement disorders
The acyl-CoA-binding domain-containing protein 6 (ACBD6) is ubiquitously expressed, plays a role in the acylation of lipids and proteins, and regulates the N-myristoylation of proteins via N-myristoyltransferase enzymes (NMTs). However, its precise function in cells is still unclear, as is the consequence of ACBD6 defects on human pathophysiology. Utilizing exome sequencing and extensive international data sharing efforts, we identified 45 affected individuals from 28 unrelated families (consanguinity 93%) with bi-allelic pathogenic, predominantly loss-of-function (18/20) variants in ACBD6. We generated zebrafish and Xenopus tropicalis acbd6 knockouts by CRISPR/Cas9 and characterized the role of ACBD6 on protein N-myristoylation with YnMyr chemical proteomics in the model organisms and human cells, with the latter also being subjected further to ACBD6 peroxisomal localization studies. The affected individuals (23 males and 22 females), with ages ranging from 1 to 50 years old, typically present with a complex and progressive disease involving moderate-to-severe global developmental delay/intellectual disability (100%) with significant expressive language impairment (98%), movement disorders (97%), facial dysmorphism (95%), and mild cerebellar ataxia (85%) associated with gait impairment (94%), limb spasticity/hypertonia (76%), oculomotor (71%) and behavioural abnormalities (65%), overweight (59%), microcephaly (39%) and epilepsy (33%). The most conspicuous and common movement disorder was dystonia (94%), frequently leading to early-onset progressive postural deformities (97%), limb dystonia (55%), and cervical dystonia (31%). A jerky tremor in the upper limbs (63%), a mild head tremor (59%), parkinsonism/hypokinesia developing with advancing age (32%), and simple motor and vocal tics were among other frequent movement disorders. Midline brain malformations including corpus callosum abnormalities (70%), hypoplasia/agenesis of the anterior commissure (66%), short midbrain and small inferior cerebellar vermis (38% each), as well as hypertrophy of the clava (24%) were common neuroimaging findings. acbd6-deficient zebrafish and Xenopus models effectively recapitulated many clinical phenotypes reported in patients including movement disorders, progressive neuromotor impairment, seizures, microcephaly, craniofacial dysmorphism, and midbrain defects accompanied by developmental delay with increased mortality over time. Unlike ACBD5, ACBD6 did not show a peroxisomal localisation and ACBD6-deficiency was not associated with altered peroxisomal parameters in patient fibroblasts. Significant differences in YnMyr-labelling were observed for 68 co- and 18 post-translationally N-myristoylated proteins in patient-derived fibroblasts. N-Myristoylation was similarly affected in acbd6-deficient zebrafish and Xenopus tropicalis models, including Fus, Marcks, and Chchd-related proteins implicated in neurological diseases. The present study provides evidence that bi-allelic pathogenic variants in ACBD6 lead to a distinct neurodevelopmental syndrome accompanied by complex and progressive cognitive and movement disorders
Effect of Different Levels of Temperature and Drought Stress on Germination Characteristics of Roman Nettle (Urtica pilulifera)
In order to investigate the effect of different levels of temperature and drought stress on seed germination parameters of nettle roman (Urtica pilulifera L.), an experiment was conducted as factorial in a completely randomized design with three replications at the Department of Agronomy, College of Agriculture, University of Guilan, in 2014. Treatments included different temperature levels (5, 10, 15, 20 and 25 °C) and drought stress levels (0 (control), -2, -4,-6 and -8 bars). The results showed that treatments and their interactions had a significant effect on germination percentage (GP), germination rate (GR), root length and root dry weight. The highest GP (88%), GR (1.8 seed/day) and root dry weight (0.06 g) were obtained as the result of the interaction between the temperature of 25°C and no-stress condition. In this study, with an increase in drought stress and a decrease in temperature, GP, GR, root dry weight, root length and shoot length decreased. The impact of drought stress on GR and GP was lower at 20°C, compared with the temperature of 25°C. A decrease in water potential from -4 to -6 bars at 20°C caused a reduction of 50% and 30% in GP and GR, respectively. However, these reductions at 25°C were 75% and 70%, respectively. According to the results, it could be stated that the significant reduction threshold of seed germination parameters was -2 bars.
Highlights:
The germination characteristics of Roman nettle were studied under drought stress and temperature.
In drought stress of -4 bars, germination percentage and germination rate decreased significantly
Assessment of Third Generation Cephalosporin (Ceftazidime and Ceftriaxone) Resistant Escherichia Coli Strains Isolated from Zahedan Hospitals by Tracing the TEM Gene
Escherichia coli is a Gram-negative, facultative anaerobic, rod-shaped bacte- rium and member of the Enterobacteriaceae family. E. coli is common in various infections, including hospital-acquired urinary tract infections. Ceftriaxone and ceftazidime are most commonly-used antibiotics to treat infections caused by Ente- robacteriaceae. The purpose of this study was to determine the antimicrobial resis- tance pattern of E. coli strains isolated from patients referred to the selected hospit- als in Zahedan by tracing the blaTEM beta-lactamase gene. Over a 12 month period, 200 clinical samples were examined. Antibiotic susceptibility was deter- mined by disk diffusion test and microdilution method and the presence of bla TEM gene was evaluated by PCR. 130 isolates were potentially extended-spectrum beta- lactamase-producing and 72 isolates contained the TEM gene. The results of the present study indicate a high rate of antibiotic resistance among E. coli isolates to ceftriaxone and ceftazidime. Therefore, it is recommended to perform antibiogram tests before prescribing antibiotic therapy. Keywords: Ecsherichia coli, Disk Diffusion Test, Extended Spectrum Beta- lactamases (ESBLs
Prediction of malaria cases in the southeastern Iran using climatic variables:An 18-year SARIMA time series analysis
Objective: To predict future trends in the incidence of malaria cases in the southeast of Iran as the most important area of malaria using Seasonal Autoregressive Integrated Moving Average (SARIMA) model, and to check the effect of meteorological variables on the disease incidence. Methods: SARIMA method was applied to fit a model on malaria incidence from April 2001 to March 2018 in Sistan and Baluchistan province in southeastern Iran. Climatic variables such as temperature, rainfall, rainy days, humidity, sunny hours and wind speed were also included in the multivariable model as covariates. Then, the best fitted model was adopted to predict the number of malaria cases for the next 12 months. Results: The best-fitted univariate model for the prediction of malaria in the southeast of Iran was SARIMA (1,0,0)(1,1,1)12 [Akaike Information Criterion (AIC)=307.4, validation root mean square error (RMSE)=0.43]. The occurrence of malaria in a given month was mostly related to the number of cases occurring in the previous 1 (p=1) and 12 (P=1) months. The inverse number of rainy days with 8-month lag ( =0.329 2) and temperature with 3-month lag ( =-0.002 6) were the best predictors that could improve the predictive performance of the univariate model. Finally, SARIMA (1,0,0)(1,1,1)12 including mean temperature with a 3-month lag (validation RMSE=0.414) was selected as the final multivariable model. Conclusions: The number of malaria cases in a given month can be predicted by the number of cases in the prior 1 and 12 months. The number of rainy days with an 8-month lag and temperature with a 3-month lag can improve the predictive power of the model.</p
Prediction of malaria cases in the southeastern Iran using climatic variables:An 18-year SARIMA time series analysis
Objective: To predict future trends in the incidence of malaria cases in the southeast of Iran as the most important area of malaria using Seasonal Autoregressive Integrated Moving Average (SARIMA) model, and to check the effect of meteorological variables on the disease incidence. Methods: SARIMA method was applied to fit a model on malaria incidence from April 2001 to March 2018 in Sistan and Baluchistan province in southeastern Iran. Climatic variables such as temperature, rainfall, rainy days, humidity, sunny hours and wind speed were also included in the multivariable model as covariates. Then, the best fitted model was adopted to predict the number of malaria cases for the next 12 months. Results: The best-fitted univariate model for the prediction of malaria in the southeast of Iran was SARIMA (1,0,0)(1,1,1)12 [Akaike Information Criterion (AIC)=307.4, validation root mean square error (RMSE)=0.43]. The occurrence of malaria in a given month was mostly related to the number of cases occurring in the previous 1 (p=1) and 12 (P=1) months. The inverse number of rainy days with 8-month lag ( =0.329 2) and temperature with 3-month lag ( =-0.002 6) were the best predictors that could improve the predictive performance of the univariate model. Finally, SARIMA (1,0,0)(1,1,1)12 including mean temperature with a 3-month lag (validation RMSE=0.414) was selected as the final multivariable model. Conclusions: The number of malaria cases in a given month can be predicted by the number of cases in the prior 1 and 12 months. The number of rainy days with an 8-month lag and temperature with a 3-month lag can improve the predictive power of the model.</p
The Cytoplasmic and Periplasmic Expression Levels and Folding of Organophosphorus Hydrolase Enzyme in Escherichia coli
This is an queer theoretical intersectional feminist study about sex workers experiences and the prejudices they face in the Netherlands. Dutch sex workers do no get the same chances and opportunities as other workers in society. So, the overarching research problem of this study is the consequences of certain discourses that confine Dutch sex workers in their lives. The thesis aims that people will critically reflect upon this study and that they will take into account the different discourses and the non-uniformity of sex workers and sex work, in other words, to create awareness and a better understanding of the complex, diverse and various groups of sex workers and the sex industry. This study consists of two research methods: literature review and interview study. The literature review consists of the previous research and the theoretical framework. The theoretical framework presents discourse and stigma as part of respectability. Discourse as systems of thinking, which effects and affects. Also this study presents respectability, in relation to the so-called non-respectable bodies of sex workers and the missing respect and dignity towards sex workers, as well as stigma as part of respectability, as the experience deviant from the normative discourse. The interview study consists of four semi-structured interviews, conducted both online and face-to-face with four Dutch participants. The method thematic content analysis was applied to code the interview transcripts and divide the content into themes and sub-themes. The three main themes are: the discourses around sex work, the consequences of the discourses around sex work and sex work as a form of labour. Under these main themes I present related sub-themes. In the analysis the participants share their stories about the negative attitude from people towards their profession, this negative attitude manifested itself in different ways. According to the participants, this negative attitude, or so-called stigma, confines sex workers in their personal and professional lives