Assessment of Nutritional Status Based on STRONGkids Tool in Iranian Hospitalized Children

Background & Objective: Malnutrition is very common in hospitalized children and is associated with related clinical consequences such as increased risk of infections, increased muscle loss, impaired wound healing, longer hospital stay and higher morbidity and mortality. The estimated prevalence of acute malnutrition in hospitalized children varies from 6.1 to 40.9% in different countries. The current study was conducted with the aim of evaluating the efficiency of STRONGkids (Screening Tool for Risk On Nutritional Status and Growth) tool for assessing malnutrition in hospitalized children in Iran. Methods: All children older than 28 days admitted to the pediatric hospital (Dr. Sheikh, Mashhad, Iran) were enrolled in this study and the screening tool named STRONGkids was applied for them. The anthropometric measurements were measured by a trained operator using standard methods and equipments. The children were classified in three groups of being at high risk, moderate risk and low risk of malnutrition. Results: According to STRONGkids score; 17% of children were classified as low risk, 75% as moderate risk and 8% as high risk group. According to WFH, HFA and WFA z-scores31.4%, 19.2% and 28% of children were identified as moderately and severely malnourished respectively. According to MUAC cut-offs, 3.4% of children were classified as having moderate malnutrition and there was no child with severe malnutrition. Conclusion: It is very important to recognize the nutritional status of the children as early as possible because of its effects on children’s growth. Therefore, evaluating the nutritional status of the hospitalized children is an essential step in clinical assessment. We suggest to apply the STRONGkids score aside with other clinical and anthropometric data

A Study of Demographic Characteristics, Clinical Manifestations, Radiologic and Lab Findings of Patients Hospitalized with COVID-19 in the South of Iran

Background & Objectives: Coronavirus (COVID-19) is a life -threatening factor all around the world. Identifying the features of the infected population contributes to detecting risk factors and determining more effective treatment goals. The purpose of this study was to investigate demographic features, clinical symptoms, and radiologic findings of hospitalized patients infected with Coronavirus (COVID-19) in Vali-Asr hospital affiliated to Fasa Medical University, located in south of Iran. Materials & Methods: In this cross-sectional study that was conducted in one of Iran southeast hospitals, 50 patients hospitalized in COVID-19 ward were selected through consensus sampling. The study's instrument included a researcher-made checklist. Then, the collected data were analyzed using SPSS software version 22 and statistical descriptive (distribution of frequency and Mean±SD ) and analytical methods (t-test). Results: The mean and standard deviation of patients’ age was 52.88±19.04. The majority of the most common clinical symptom was fever and the most common CTs finding was the GGO (Grand Glossy Opacity) engagement of both lungs. There was no meaningful difference between age, gender, and infection with coronavirus, respectively (P=0.87 and P=0.79). Among the underlying diseases, there was a significant relationship between Chronic Obstructive Pulmonary Disease (COPD) and coronavirus infection (P=0.003). Conclusion: Old age and background diseases (diabetes, chronic obstructive pulmonary disease) are factors that endanger people to COVID-19 more. It is suggested to carry out more studies to investigate the effect of the characteristics of different communities on the infection with COVID-19

Recent Progress in Fill Media Technology for Wet Cooling Towers

Cooling towers are extensively utilized in diverse industries for efficient heat dissipation. Fill media are a critical component, facilitating heat and mass exchange between water and air, impacting overall cooling tower efficiency. Given its vital importance, this study comprehensively reviews recent advancements in fill media technology, illuminating cooling tower technology progress and exploring the effects of different fill media configurations and materials on cooling tower performance. It should be noted that the majority of research is focused on the Range of 2.5 °C to 25 °C and Approach of 1 °C to 9 °C. Through comprehensive analysis and evaluation, the effects of various fill media on heat transfer efficiency, water cooling capacity, and energy consumption are intensively summarized. By understanding these effects, engineers and designers can make rational decisions to optimize cooling tower performance and ensure efficient heat dissipation. Notably, in some reported cases, new fill media enhanced cooling range, effectiveness, and the Merkel number by 28%, 85%, and 131%, respectively. Ultimately, this paper serves as a valuable resource for academics, researchers, and professionals in the field of cooling tower design and thermal management. The insights provided in this study can help industries achieve greater energy efficiency, sustainability, and overall operational excellence

Exploring the association between serum Vitamin D levels and the development of coronary artery lesions in Kawasaki disease - a systematic review

Abstract Background Kawasaki Disease (KD) involves arterial inflammation, primarily affecting the coronary arteries and leading to coronary artery lesions. Recent advancements in understanding the immunomodulatory roles of vitamin D have prompted investigations into the potential correlation between serum vitamin D levels and the risk of coronary artery lesions (CAL) in KD. This review aims to explore this association. Methods A systematic search utilizing relevant keywords related to Kawasaki disease and coronary artery lesions was conducted across four databases (PubMed, Embase, Scopus, and Web of Science). The quality of the incorporated studies was assessed utilizing the Newcastle-Ottawa Scale. The study protocol is registered in PROSPERO under the registry code CRD42024493204. Results In a review of five studies involving 442 KD patients and 594 healthy controls, KD patients generally had lower serum vitamin D levels compared to controls, with mixed findings on the association with coronary artery lesions and IVIG resistance. While three studies supported lower vitamin D in KD, one showed no significant difference. Regarding CAL, one study found lower vitamin D, another found higher levels associated with CAL, and two found no significant difference. Conclusions Overall, the evidence is inconclusive, but there’s a trend suggesting potential benefits of sufficient vitamin D levels in Kawasaki disease rather than evidence refuting any association with clinical outcomes

Application of High-Throughput Sequencing for Comprehensive Virome Profiling in Grapevines Shows Yellows in Iran

A comprehensive study on the whole spectrum of viruses and viroids in five Iranian grapevine cultivars was carried out using sRNA libraries prepared from phloem tissue. A comparison of two approaches to virus detection from sRNAome data indicated a significant difference in the results and performance of the aligners in viral genome reconstruction. The results showed a complex virome in terms of viral composition, abundance, and richness. Thirteen viruses and viroids were identified in five Iranian grapevine cultivars, among which the grapevine red blotch virus and grapevine satellite virus were detected for the first time in Iranian vineyards. Grapevine leafroll-associated virus 1 (GLRaV1) and grapevine fanleaf virus (GFLV) were highly dominant in the virome. However, their frequency and abundance were somewhat different among grapevine cultivars. The results revealed a mixed infection of GLRaV1/grapevine yellow speckle viroid 1 (GYSVd1) and GFLV/GYSVd1 in grapevines that exhibited yellows and vein banding. We also propose a threshold of 14% of complete reconstruction as an appropriate threshold for detection of grapevine viruses that can be used as indicators for reliable grapevine virome profiling or in quarantine stations and certification programs

Evaluation of the Expression of Infection-Related Long Noncoding RNAs among COVID-19 Patients: A Case-Control Study

Background and Aims. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a worldwide pandemic, activates signaling cascades and leads to innate immune responses and secretion of multiple chemokines and cytokines. Long noncoding RNAs (lncRNAs) have a crucial role in inflammatory pathways. Through our search on the PubMed database, we discovered that existing research has primarily focused on examining the regulatory impacts of five lncRNAs in the context of viral infections. However, their role in regulating other conditions, including SARS-CoV-2, has not been explored. Therefore, this study aimed to investigate the expression pattern of lncRNAs in the peripheral blood mononuclear cells (PBMC) and their potential roles in SARS-CoV-2 infection. Potentially significant competing endogenous RNA (ceRNA) networks of these five lncRNAs were found using online in-silico techniques. Methods. Ethylenediaminetetraacetic acid (EDTA) blood samples of the control group consisted of 45 healthy people, and a total of 53 COVID-19-infected patients in case group, with a written informed consent, was collected. PBMCs were extracted, and then, the RNA extraction and complementary DNA (cDNA) synthesis was performed. The expression of five lncRNAs (lnc ISR, lnc ATV, lnc PAAN, lnc SG20, and lnc HEAL) was assessed by real-time PCR. In order to evaluate the biomarker roles of genes, receiver operating characteristic (ROC) curve was drawn. Results. Twenty-four (53.3%) and 29 (54.7%) of healthy and COVID-19-infected participants were male, respectively. The most prevalent symptoms were as follows: cough, general weakness, contusion, headache, and sore throat. The results showed that three lncRNAs, including lnc ISR, lnc ATV, and lnc HEAL, were expressed dramatically higher in the case group compared to healthy controls. According to ROC curve analysis, lnc ATV has a higher AUC and is a better biomarker to differentiate COVID-19 patients from the healthy controls. Then, using bioinformatics methods, the ceRNA network of these lncRNAs enabled the identification of mRNAs and miRNAs with crucial functions in COVID-19. Conclusion. The considerable higher expression of ISR, ATV, and HEAL lncRNAs and the significant area under curve (AUC) in ROC curve demonstrate that these RNAs probably have a potential role in controlling the host innate immune responses and regulate the viral replication of SARS-CoV-2. However, these assumptions need further in vitro and in vivo investigations to be confirmed

Short-term effects of particle size fractions on circulating biomarkers of inflammation in a panel of elderly subjects and healthy young adults

Systemic inflammation biomarkers have been associated with risk of cardiovascular morbidity and mortality. We aimed to clarify associations of acute exposure to particulate matter (PM10 (PM < 10 μm), PM2.5-10 (PM 2.5-10 μm), PM2.5 (PM < 2.5 μm), PM1-2.5 (PM 1-2.5 μm), and PM1 (PM < 1 μm)) with systemic inflammation using panels of elderly subjects and healthy young adults. We followed a panel of 44 nonsmoking elderly subjects living in a retirement home and a panel of 40 healthy young adults living in a school dormitory in Tehran city, Iran from May 2012 to May 2013. Blood biomarkers were measured one every 7-8 weeks and included white blood cells (WBC), high sensitive C-reactive protein (hsCRP), tumor necrosis factor-soluble receptor-II (sTNF-RII), interleukin-6 (IL-6), and von Willebrand factor (vWF). We measured hourly indoor and outdoor exposure to PM10, PM2.5-10, PM2.5, PM1-2.5, and PM1 mass concentration to derive weighted averages of personal exposure based on simultaneously collected time-activity data. The random intercept linear mixed effects model was used for data analysis. We observed significant positive associations for WBC and IL-6 with exposure to PM10, PM2.5-10, PM2.5, PM1-2.5, and PM1; sTNF-RII with PM2.5, PM1-2.5, and PM1; hsCRP with PM2.5 and PM1; and vWF with PM10 and PM2.5-10, PM2.5, and PM1-2.5 mass concentration in elderly subjects from the current-day and multiday averages. For healthy young adults, we found significant positive associations for WBC and IL-6 with exposure to PM10, PM2.5-10, PM2.5, and PM1-2.5, but no with PM1. The results showed that increase of hsCRP, sTNF-RII, and vWF were not significantly associated with any of the PM sizes investigated in the healthy young subjects. Our results provided some evidence that short-term exposure to PM10, PM2.5-10, PM2.5, PM1-2.5, and PM1 was associated with inflammation and coagulation blood markers, but associations were depended on PM size and also differed across the various time lag

Bioaerosol exposure and circulating biomarkers in a panel of elderly subjects and healthy young adults

Numerous studies have found that risk of cardiovascular diseases is associated with increased blood levels of circulating markers of systemic inflammation. We investigated associations of acute exposure to bioaerosols (bacteria and fungi) with blood markers of inflammation and coagulation using panels of elderly subjects and healthy young adults. We conducted a panel study of 44 nonsmoker elderly subjects in a retirement communities and a panel study of 40 healthy young adults living in a school dormitory within Tehran city, Iran. Blood sample biomarkers were measured weekly over 6 weeks and including high sensitive C-reactive protein (hsCRP), tumor necrosis factor-soluble receptor-II (sTNF-RII), von Willebrand factor (vWF), white blood cells (WBC) count and interleukin-6 (IL-6). We found significant positive associations for IL-6 and WBC with exposure to Aspergillus spp. (As), Cladosporium spp. (Cl), Penicillium spp. (Pe), total fungi (TF) and Micrococcus spp. (MI); vWF with Cl and MI; sTNF-RII with Staphylococcus spp. (ST) in healthy young adults from the current-day and multiday averages. For elderly subjects, we observed significant positive associations for hsCRP, sTNF-RII and WBC with exposure to MI, but not with ST and total bacteria (TB). Our results showed the strongest significant positive associations for IL-6 with MI, ST and TB in elderly people. In addition, IL-6 was also positively associated with As, Cl and Pe in elderly. Also, the results showed that increase of vWF was significantly associated with bacterial and fungal aerosols, except Bacillus spp. (BA) at some lags in elderly subjects. Pooled results support the pivotal role of bioaerosols in increasing the level of some of inflammatory biomarkers, especially IL-6 and WBC in healthy young adults but possibly also in elderly people.</p

Rearing and Biology of Phlebotomus sergenti, the Main Vector of Anthroponotic Cutaneous Leishmaniasis in Iran

Background: Establishment of sand flies laboratory colonies is essential to understand various biological aspects of Phlebotominae sand flies. The aims of the current study were to establish the colony of Phlebotomus sergenti Parrot (1917), the main vector of anthroponotic cutaneous leishmaniasis in old world, and to study biological parameters of this species. Methods: The sand flies were reared at 26–28 °C temperature, 14:10 (light: dark) photoperiod and 70–80% relative humidity. Larval diet was a composted mixture of rabbit faces and rabbit pellets which is prepared through a special process. First to fifth generations of P. sergenti were used to define biological parameters. Results: Results showed that, P. sergenti blood feeding percentage were 42% on chicken, 21% on BALB/c and 37% on golden hamster. Average time of blood digestion, egg incubation, 1st instar larva, pupa and adult emerging was recorded at 3.4, 8.7, 15, 33.3 and 41.2 days after blood feeding, respectively. Mean number of laid eggs was 55.1 and retained eggs were 35 per a female. Fecundity and production rate were 61.6%, and 42.2% respectively. Average longevity recorded at 15.2 days for females and 14.8 days for males. Conclusion: Colony of P. sergenti has been established for the first time in Iran. Average interval time from egg to adult of this species was 32.5 days. Chicken and golden hamster were recommended as a blood source for colony initiation and routine blood feeding, respectively.