Additional file 1: Figure S1. of Influence of weaning methods on the diaphragm after mechanical ventilation in a rat model

Arterial blood gas data in the CMV, PSV and SBT groups. Values are expressed as means ± SD. (JPG 717 kb

Diaphragmatic Outcomes.

<p>A. Transdiaphragmatic pressure measurement: airflow recording showing tracheostomy occlusion with halt of airflow (red arrow), increase in Pdi (black arrow). B. Pdi increases in Sham and Gore-Tex implanted animals following tracheostomy occlusion, C. Increase in Pdi as a % change at rest and following occlusion, D. Right hemi diaphragmatic muscle fiber cross sectional (μm²) area is increased in Gore-Tex implanted animals. <i>Poes</i>: <i>oesophageal pressure</i>, <i>Pab</i>: <i>abdominal pressure</i>, <i>Pdi</i>: <i>transdiaphragmatic pressure</i>.</p

Pulmonary Outcomes.

<p>Chest X-Ray (CXR) showing A. Cobb angle measurement (PA film) B. lateral view in sham operated animals and pulmonary function testing showing C. Flow rate, d. Tidal volume.</p

Diaphragmatic contractile properties.

<p>Diaphragmatic contractile properties.</p

Creation and repair of a left diaphragmatic defect in a 6-week old rabbit.

<p>A. Operative position, B. Left subcostal transverse incision, C. Division of the posterior triangular ligament of the liver, D. Subtotal left diaphragmatic excision with Gore-Tex mesh repair, E. 3*3cm diaphragmatic defect, F. Repair with a 3,5*3,5cm Gore-Tex patch G. Sub-cuticular skin closure.</p

4 HNE / oxyblot / alpha-spectrin

Western blot

Prolonged mechanical ventilation worsens sepsis-induced diaphragmatic dysfunction in the rat - Fig 1

<p><b>Maximal tetanic force (Panel A), maximal twitch force (Panel B) and force-frequency relationship (Panel C) of the diaphragm in the four experimental groups.</b> Control = spontaneous ventilation without endotoxemia; MV = mechanical ventilation without sepsis; SV-LPS = endotoxemia with spontaneous ventilation; MV-LPS = endotoxemia with mechanical ventilation. Results are presented in the form of box plots. Boxes are drawn between the first and third quartiles of the distribution, black bars indicate the median, and whiskers indicate the minimum and maximum values, n = 8 per group. *: P<0.05 versus Control; †: P<0.05 versus MV; $: P< 0.05 versus SV-LPS.</p

Mean arterial blood pressure and arterial blood gases at the end of the 12-h protocol.

<p>Mean arterial blood pressure and arterial blood gases at the end of the 12-h protocol.</p

Prolonged mechanical ventilation worsens sepsis-induced diaphragmatic dysfunction in the rat - Fig 4

<p><b>Relationship between maximum isometric tetanic force of the diaphragm and tumor necrosis factor (TNF)-α (Panel A), interleukin (IL)-1β (Panel B) and IL-6 (Panel C) protein concentration in the diaphragm (left panels) and in the plasma (right panels).</b> Correlation determined by Spearman test.</p

Prolonged mechanical ventilation worsens sepsis-induced diaphragmatic dysfunction in the rat - Fig 3

<p><b>Tumor necrosis factor (TNF)-α (Panel A), interleukin (IL)-1β (Panel B) and IL-6 (Panel C) protein concentration in the diaphragm (left panels) and in the plasma (right panels) in the four experimental groups.</b> Control = spontaneous ventilation without endotoxemia; MV = mechanical ventilation without sepsis; SV-LPS = endotoxemia with spontaneous ventilation; MV-LPS = endotoxemia with mechanical ventilation. Results are presented in the form of box plots as well as individual values. Boxes are drawn between the first and third quartiles of the distribution, black bars indicate the median, and whiskers indicate the minimum and maximum values, n = 8 per group. *: P<0.05 versus Control; †: P<0.05 versus MV; $: P< 0.05 versus SV-LPS.</p