Evaluation of Naked Barley Landraces for Agro-morphological Traits

Naked barley (Hordeum vulgare var. nudum L.) is a traditional, culturally important, climate-resilient winter cereal crop of Nepal. Evaluation of the naked barely genotypes for yield and disease is fundamental for their efficient utilization in plant breeding schemes and effective conservation programs. Therefore, to identify high yielding and yellow rust resistant landraces of naked barley for hilly and mountainous agro-ecosystem, twenty naked barley landraces collected from different locations of Nepal, were evaluated in randomized complete block design (RCBD) with three replications during winter season of 2016 and 2017 at Khumaltar, Lalitpur, Nepal. Combined analysis of variances revealed that NGRC04902 (3.46 t/ha), NGRC00886 (3.28 t/ha), NGRC02309 (3.21 t/ha) and NGRC06026 (3.10 t/ha) were the high yielding landraces and statistically at par with the released variety 'Solu Uwa' (3.15 t/ha). The landraces namely NGRC00837 (ACI Value: 1.86) was found resistant to yellow rust diseases. Landraces NGRC06034 (131.7 days) and NGRC02363 (130.8 days) were found early maturing and NGRC02306 (94.36 cm) was found dwarf landraces among tested genotypes. These landraces having higher yield and better resistance to yellow rust need to be deployed to farmers' field to diversify the varietal options and used in resistant breeding program to improve the productivity of naked barley for Nepalese farmers

Agro-morphological Diversity of High Altitude Bean Landraces in the Kailash Sacred Landscape of Nepal

Many varieties of bean are widely grown across diverse agro-ecological zones in Nepal. And opportunities exist for improving the crops and enhancing their resilience to various biotic and abiotic stressors. In this context, an experiment was conducted from June to October 2016 in Khar VDC of Darchula district to study the phenotypic traits of nine landraces of bean (Phaseolus vulgaris L.). The bean landraces were planted using randomized complete block design in three sites (Dhamidera, Dallekh and Sundamunda villages), with three replications in each site for their comparative analysis. The study considered the following phenotypic traits: days to emergence, days to 50% flowering, days to 90% pod maturity, number of nodes, pod length, pod width, number of pods, number of seeds per pod and weight and grain yield for 100 seeds. Kruskal-Wallis test showed significant differences in the landraces both within and among locations. KA-17-08-FB and KA-17-04-FB were late  flowering (63 and 65 days respectively) compared to other landraces whereas KA-17-07-FB flowered earliest (within 42 days). In all three sites, three landraces namely KA-17-07-FB, KA-17-04-FB and KA-17-06-FB were found to be relatively more resistant to pest and diseases than other landraces. Eight out of nine landraces in Dhamidera and Dallekh villages and seven out of nine in Sundamunda village produced seeds greater than 1.0 t/ha. Among the nine varieties KA-17-02-FB was the highest yielding variety, with an average yield of 3.8 t/ha. This study is useful for identifying suitable landraces for future promotion based on their maturity, grain yield, diseases resistance and other qualitative and quantitative characteristics

Plant density and life history traits of <i style="">Aconitum spicatum</i> in North-central Nepal:effects of elevation and anthropogenic disturbances

Increasing cross-border trade of medicinal and aromatic plants (MAPs) has put heavy pressure on a considerable number of species in the Himalayas. One of the threatened species in Nepal is Aconitum spicatum. Unfortunately for this species and for many others, our knowledge on population ecology and performance across the distribution range is insufficient, hindering the formulation of species-specific management plans. We therefore studied density and population structure of A. spicatum and assessed variation in its life history traits among three populations (subalpine, lower alpine and alpine) along an elevation gradient (3,000–4,200 m a.s.l.) in Annapurna Conservation Area, north-central Nepal. The results show that human disturbances and topographic factors contributed to the variation in density and life history traits. The overall density ranged between 0.56 ± 0.09 (Mean ± SE) and 2.48 ±  0.24 plants/m2 with highest mean density in the lower alpine and lowest in the subalpine population. The subalpine population was also characterized by lower investment in reproductive structures with lowest seed mass and low seed viability and fecundity. Among the environmental variables tested, harvesting, animal droppings and fire appeared to be the most important factors affecting density of different life stages of A. spicatum. The prevailing harvesting pattern is destructive as it involves uprooting of the whole plant and this appears to be a main reason for low recruitment and reduced density of the subalpine population. The level of disturbance decreased with increasing elevation. In terms of reproductive effort, the alpine population performed best. Our results indicate that the viability of A. spicatum populations depends on controlling over-harvesting and pre-mature harvesting of tubers and protecting younger life stages from grazing, trampling and fire. We therefore recommend that when formulating management guidelines, measures aiming to mitigate such anthropogenic disturbances should be considered

Assessment of Pesticide Use in Major Vegetables From Farmers\u27 Perception and Knowledge in Dhading District, Nepal

A field study was carried out to assess the pesticide use status in major vegetable crops from farmers\u27 perception and knowledge in Dhading, Nepal in 2019. Field study was carried with 100 commercial farmer\u27s using semi-structure questionnaire by face to face interview. This study was analyzed by categorization of farmers into small holder (51) and large holder (49) groups on the basis of mean area of vegetable cultivation (6.48 ropani). The highest amount of pesticides is needed in tomato in both large holders and small holders according to the farmer\u27s experience. Among the study farmer\u27s, 41% of them spray the pesticides by making a cocktail or mixed method and 56%&nbsp; follow the waiting period of 3-5 days in both of the cases. A significant positive correlation was found at 5% level of significance between the knowledge and practice pattern of waiting period of the pesticides and negative correlation was found between the Personal Protective equipment score and health hazard score. Headache was the major health hazards faced by the farmers which was higher in small holders (66.7%) as compared to the large holders (46.9%). Mask was the most used PPE by the farmer\u27s i.e. by 83% in overall. Fourty three percent of the farmer\u27s throw the pesticide containers in secret place after using of it.The use of PPE was seen lower in small holders as compared to the large holders. This study reveals the necessities of suitable program and policies regarding the knowledge, safe handling and use of pesticide among the farmer\u27s level

Genetic Relationship among Nepalese Rice Landraces and Cultivars based on RAPD Markers

Genetic information of any genotype is necessary to manage and utilize them in conservation and breeding program. A total of 28 RAPD markers were used to relate the genetic structure among 50 Nepalese rice genotypes consisting of 29 landraces, 12 breeding lines and 9 released cultivars. Some of them are aromatic and blast resistance. Only four primers (P41, P60, P109 and P141) amplified the DNA of these genotypes with scorable bands. Primer 60 produced the highest number of bands (8). The highest number of present bands (6) was shown by primer 41 in 10 rice genotypes. Grouping of these genotypes based on the adaptation to agro-climatic zone was not observed, probably due to low percentage coverage of genome by four primers.&nbsp; Most of the genotypes grouped in two clusters. Kali Marsi and IR-24 formed separate individual cluster. Mansara and Jarneli were the most similar landraces (0.96). Churenodhan and Pranpyuri were the most closely related with Masuli. Only one genotype NR-285-18 has fallen in the first quadrant by principal component (PC) analysis and the fourth quadrant was empty. The highest contribution in PC1 was from the second band of primer 41. This RAPD information can be used for selecting lines and for blast resistance breeding.Key words: Genetic distance; rice; RAPDDOI: http://dx.doi.org/10.3126/njb.v2i1.5635Nepal Journal of Biotechnology Jan.2012, Vol.2(1): 16-2