The Effects of Resveratrol, Metformin, Cold and Strength Training on the Level of Perilipin 5 in the Heart, Skeletal Muscle and Brown Adipose Tissues in Mouse

The high accumulation of lipid droplets in the cell is related to metabolic disorders, such as obesity. Perilipin 5 (Plin5), plays an important role in triglyceride hydrolysis in the lipid droplets. In this study, this protein has been evaluated in different tissues and conditions in mice. Fifty male mice were divided into 5 groups and treated for 45 days with Resveratrol, Metformin, strength training, and 4 °C cold. Brown adipose tissue (BAT), gastrocnemius skeletal muscle and heart were isolated for RNA extraction. The Plin5 gene expression was evaluated, using Real-Time PCR, and the plin5 was analyzed at the protein level, using western blot. In BAT, Resveratrol significantly reduced the plin5 protein level and gene expression (p < 0.05). In heart tissue, Resveratrol and strength training, decreased (p < 0.05) the plin5 expression, but Metformin increased the gene expression (p < 0.05). In skeletal muscle, resveratrol, strength training, cold and Metformin significantly increased the plin5 expression at the gene and protein level (p < 0.05). In BAT, Resveratrol has a greater effect in decreasing lipid deposits, compared with the strength training and cold; thus, it can play a better role in preventing lipid accumulation. In heart tissue, Resveratrol probably decreases insulin resistance, due to the increased expression of plin5 in skeletal muscl

The Relationship of Serum Levels of Gamma Interferon and its Receptor (CD119) to Development of BCG Axillary Lymphadenopathy

Background: Lymphadenitis is the most common complication of BCG vaccination in children. Interferon-gamma (IFN-gamma) plays a key role in immune response to Mycobacterial infections. In this study, the relationship of serum levels of IFN-gamma and its receptor (CD119) to development of Bacillus Calmette Guerin (BCG) axillary lymphadenopathy was investigated. Materials and Methods: In this case-control study, 45 children with axillary lymphadenopathy and 45 healthy children matched by age and sex were included. Two ml peripheral blood was collected in tubes containing anticoagulants. Then, level of IFN-gamma was measured by ELISA and the level of CD119 expression in the peripheral blood mononuclear cell (PBMC) was measured by flow cytometry. Data were analyzed using SPSS software version 22.0. Results: Totally, 90 children were enrolled in this study, which consisted of 30 girls and 60 boys. The mean age of participants was 14.5 +/- 6.5 months in case group and 15.2 +/- 7.1 months in control group. respectively (p=0.61). The level of IFN-gamma was significantly lower in case group than in control group (p0.05). There was no significant relationship of age and sex to BCG (INF and PBMC) lymphadenopathy (p>0.05). Conclusion: Based on the results. IFN-gamma level was significantly lower in the BCG lymphadenopathy group than in the control group. Levels of IFN-gamma R (CD119 cellular level in PBMC) in two groups did not show a significant relationship. Keywords:Bacillus Calmette Guerin; CD119; Children; Interferon gamma; Lymphadenopath

Seroepidemiology of Toxocariasis in Children (5-15 yr Old) Referred to the Pediatric Clinic of Imam Hossein Hospital, Isfahan, Iran.

BACKGROUND: Human toxocariasis, a helminthozoonosis, is due to the migration of Toxocara species larvae into human organisms. Humans, especially children become infected by ingesting of embryonated eggs from soil, dirty hands, and raw vegetables. Seroprevalence of this infection is high in developed countries, especially in rural areas. The aim of this study was to investigate the seroepidemiology of Toxocariasis in children referred to the pediatric clinic of Imam Hossein hospital, Isfahan, Iran. METHODS: In this cross sectional study the sera of children aged 5 to 15 years old, admitted to Imam Hossein Pediatric Hospital were collected during 2013-14. Then the sera were examined for anti Toxocara canis antibodies using commercial ELISA kit. RESULTS: From 427 children, 196 (45.9%) were female and 231(54.1%) were male. 107(25.1%) were from rural and 320 (74.9%) were from urban area. Of them 129 (30.2%) were contacted with dog. One child (0.2%) had hypereosinophilia, 33 (7.7%) eosinophlia, and 6 (1.39%) were positive for T. canis IgG (two male and four female). Four of infected children with T. canis were from urban (1.25%) and two from rural areas (1.9%). There was no significant correlation between education of parents, gender, age, place of living and contact with dog with ELISA results test. CONCLUSION: Toxocariasis is prevalent in the children of Isfahan region. Results suggest a low Toxocara exposure in children in this area. Therefore, more risk factors associated with Toxocara exposure should be identified in the further investigatio

Evaluation of the Effects of Photobiomodulation on Partial Osteotomy in Streptozotocin-Induced Diabetes in Rats

Objective: We examined the effects of photobiomodulation (PBM) on stereological parameters, and gene expression of Runt-related transcription factor 2 (RUNX2), osteocalcin, and receptor activator of nuclear factor kappa-B ligand (RANKL) in repairing tissue of tibial bone defect in streptozotocin (STZ)-induced type 1 diabetes mellitus (TIDM) in rats during catabolic response of fracture healing. Background data: There were conflicting results regarding the efficacy of PBM on bone healing process in healthy and diabetic animals. Materials and methods: Forty-eight rats have been distributed into four groups: group 1 (healthy control, no TIDM and no PBM), group 2 (healthy test, no TIDM and PBM), group 3 (diabetic control, TIDM and no PBM), and group 4 (diabetic test, no TIDM and PBM). TIDM was induced in the groups 3 and 4. A partial bone defect in tibia was made in all groups. The bone defects of groups second and fourth were irradiated by a laser (890 nm, 80 Hz, 1.5 J/cm2 ). Thirty days after the surgery, all bone defects were extracted and were submitted to stereological examination and real-time polymerase chain reaction (RT-PCR). Results: PBM significantly increased volumes of total callus, total bone, bone marrow, trabecular bone, and cortical bone, and the numbers of osteocytes and osteoblasts of callus in TIDM rats compared to those of callus in diabetic control. In addition, TIDM increased RUNX2, and osteocalcin in callus of tibial bone defect compared to healthy group. PBM significantly decreased osteocalcin gene expression in TIDM rats. Conclusions: PBM significantly increased many stereological parameters of bone repair in an STZ-induced TIDM during catabolic response of fracture healing. Further RT-PCR test demonstrated that bone repair was modulated in diabetic rats during catabolic response of fracture healing by significant increase in mRNA expression of RUNX2, and osteocalcin compared to healthy control rats. PBM also decreased osteocalcin mRNA expression in TIDM rats

Study of three common ApoB gene mutations in possible familial hypercholesterolemia patients in Cheharmahal va Bakhtiari province, Iran, 2003

Background and aim: Familial hypercholesterolemia (FH) is an autosomal dominant disorder caused mainly by mutations in the low-density lipoprotein receptor (LDLR) and apolipoprotein B (ApoB).Until now, the molecular basis of FH has been demonstrated in detail in many populations , but there is still very limited molecular data concerning FH in Iran . The aim of this study was to investigate frequency of 3 common Apo-B100 gene mutations in an Iranian population. Method: In this descriptive –lab based study a total of 30 non-related possible FH subjects from Cheharmahal va Bakhtiari were studies. All samples were tested for 3 common Apo-B100 gene mutations including R3500Q, R3500W and R3531C using PCR-RFLP procedure. Result: None of the common Apo-B100 mutations R3500Q, R3500W and R3531C were detected in samples examined using PCR-RFLP. Conclusion: Our data indicated that Apo-B100 gene mutations have not contribution to FH in samples studied here. However, we examined 3 common Apo-B100 mutations in only 30 patients, and to determine the role of mutations of this gene in developing FH in Cheharmahal va Bakhtiari province, more FH samples/populations needed to be investigated

Molecular characterization of familial hypercholesterolemia in Iranian patients

Abstract Familial hypercholesterolemia (FH) is an autosomal dominant disorder of lipoprotein metabolism caused mainly by mutations in the low-density lipoprotein receptor (LDLR) and apolipoprotein B 100 (APOB) genes. Until now, the molecular basis of FH has been demonstrated in detail in many populations, but there is still very limited Molecular data concerning FH in Iran. The aim of this study was to characterize the LDLR and APOB gene mutations in an Iranian population. A total of 30 non-related Iranian possible FH subjects were studied. Diagnosis of FH was based on the Dutch Lipid Clinic Network diagnostic criteria. All samples were initially tested for three common APOB gene mutations including R3500Q, R3500 W and R3531C using PCR-RFLP assay. Subsequently, promoter and coding region of the LDLR gene was screened by PCRSSCP analysis and positive results were confirmed by DNA sequencing. Four previously reported polymorphisms 1413G [A, 1725C [T, 1773T [C and 2140 ? 5G[A were found in *17% (5/30) of population studied. Moreover, no variation was found in APOB gene. Our data indicated that LDLR and APOB gene mutations have not contribution to possible FH in Iranian population studied here. However, we examined three common APOB mutations and LDLR in only 30 patients, and to determine the role of these genes in developing FH in Iran, more FH samples and populations needed to be investigated for the mutations of the related gene

CAN RELAXATION BE USED TO ACHIEVE WEIGHT CONTROL IN YOUTHS?

&nbsp; Abstract INTRODUCTION: Childhood obesity is a cultural and medical problem and is usually a refractory to treatment. The objective of this study was to assess the effects of parental therapy behavior and relaxation methods on children in controlling childhood obesity. methods: In this randomized controlled clinical trial, 90 obese children aged 10-17 years were randomly divided into three groups of equal number. All three groups received similar diet and exercise recommendations. The first group of children attended 15 relaxation sessions. Parents of the second group participated in 15 behavior therapy sessions. The third group was considered as control. Data were analyzed by SPSS&nbsp;13. Mean changes of weight, BMI and WC were calculated. results: Mean BMI decreased in all three groups after the intervention. This decrease was more remarkable, but not significant in the first group. Mean weight decreased in the first group but increased in the second group. Mean WC decreased in all three groups. This decrease was more obvious, but not significant in the first group. CONCLUSIONS: Stress leads to sympathetic system arousal which it associated with many pathologic conditions. Elicitation of relaxation response can help individuals embrace healthy lifestyle choices. Relaxation and parent behavior therapy can be considered as useful methods of controlling childhood obesity. &nbsp; &nbsp; Keywords: Obesity, children and adolescents, relaxation, behavior therapy, parents.</div

The protocol of a population-based prospective cohort study in southwest of Iran to analyze common non-communicable diseases: Shahrekord cohort study

Abstract Background Prospective cohort studies are considered ideal choices to study multiple outcomes and risk factors for Non-communicable diseases (NCDs). Our aim is to set-up the protocol and analyze risk factors, incidence rates, prevalence, trends, and the models of environmental and genetic determinants of NCDs and their outcomes as well as interaction among such determinants. Methods Shahrekord cohort study (SCS) that is a population-based prospective, study on a cohort consisting of people aged 35-70 years started in November 2015 in Iran. The sample size of the original cohort is at least 10,000 people. Annual follow-ups (200,000 person-year) of the cohort were designed to be conducted up to 2036. Exposures (a detailed demographic, socioeconomic, general health, quality of life, physical activity, anthropometric indexes, stress, health literacy, social capital, nutrition and eating habits, lifestyle, occupational history, living place, blindness, deafness, electrocardiography, lung capacities, blood pressure, sleep, smoking and alcohol, contact to animals, physical examinations and medical history, dental health, used drugs and supplements, glucose and lipid profiles) were measured by relevant standard methods and questionnaires. Incidence of common NCDs (cardiovascular diseases, cancer, gastrointestinal, respiratory, renal, hepatic, accidents, injury and neurological diseases), trend of risk factors, hospitalization, disability, and death were considered the outcomes of the cohort. The definition of disease was determined based on the International Classification of Diseases 10th version (ICD-10). Routine hematologic and biochemical tests were conducted and an all-inclusive biobank (blood, hair, nail, and urine specimens) of the cohort was stored for future studies. All steps of data collection and examinations are directly monitored by the quality control team. Discussion The SCS is a unique study conducted in southwest of Iran that is a notable work given the climate conditions and ethnicity population (especially in Bakhtiari) of this region. By providing high quality the protocol and introduce it, the SCS can serve as a solid foundation for management and researchers in southwest of Iran. The SCS provides prerequisites for collaboration and regional, national, and international studies on NCDs. Data are available at the modeling in health research center, Shahrekord University of Medical Sciences, Shahrekord, Iran, for any collaboration

Intensified Th9 Response is Associated with the Immunopathogenesis of Active Ulcerative Colitis

Abstract Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the large intestine histologically characterized by indistinct sustained inflammatory responses. Genetical susceptibility and environmental factors’ effects play the roles in disease occurrence and it can be life threatening if remains untreated. It seems that intensification of inflammatory responses in this condition is not restricted to a specific cell line of T lymphocytes. Our aim was to determine the number of T helper 9 (Th9) cells in inflamed colonic biopsies of UC patients. We also correlated it with interleukin (IL)-9 protein level in addition to certain genes expressions associated with Th9 phenotype. Methods: Expression of CD4 and IL-9 were evaluated by immunohistochemical staining. Enzyme linked immunosorbent assay (ELISA) was performed to determine the colonic expression of IL-9 protein and finally mRNA expressions of interferon regulatory factor 4 (Irf4), Smad2, and Smad3 were measured by real-time polymerase chain reaction (RT-PCR) as critical transcription factors of Th9 differentiation. Results: Number of Th9 cells was significantly increased in inflamed samples as compared with normal tissues. Also quantitative measurement of IL-9 by ELISA and mRNA expressions of Irf4, Smad2, and Smad3 showed notable correlative enhancements in patient’s samples. Conclusion: Function and number of Th9 cells are up-regulated in the inflamed mucosa of UC patients as with the protein secretion of IL-9 and mRNA expressions of Irf4, Smad2, and Smad3, so Th9 cells and IL-9 may become remarkable therapeutic targets for IBD treatment in the future. © 2018 Taylor & Franci

The protocol of a population-based prospective cohort study in southwest of Iran to analyze common non-communicable diseases: Shahrekord cohort study

Background: Prospective cohort studies are considered ideal choices to study multiple outcomes and risk factors for Non-communicable diseases (NCDs). Our aim is to set-up the protocol and analyze risk factors, incidence rates, prevalence, trends, and the models of environmental and genetic determinants of NCDs and their outcomes as well as interaction among such determinants. Methods: Shahrekord cohort study (SCS) that is a population-based prospective, study on a cohort consisting of people aged 35-70 years started in November 2015 in Iran. The sample size of the original cohort is at least 10,000 people. Annual follow-ups (200,000 person-year) of the cohort were designed to be conducted up to 2036. Exposures (a detailed demographic, socioeconomic, general health, quality of life, physical activity, anthropometric indexes, stress, health literacy, social capital, nutrition and eating habits, lifestyle, occupational history, living place, blindness, deafness, electrocardiography, lung capacities, blood pressure, sleep, smoking and alcohol, contact to animals, physical examinations and medical history, dental health, used drugs and supplements, glucose and lipid profiles) were measured by relevant standard methods and questionnaires. Incidence of common NCDs (cardiovascular diseases, cancer, gastrointestinal, respiratory, renal, hepatic, accidents, injury and neurological diseases), trend of risk factors, hospitalization, disability, and death were considered the outcomes of the cohort. The definition of disease was determined based on the International Classification of Diseases 10th version (ICD-10). Routine hematologic and biochemical tests were conducted and an all-inclusive biobank (blood, hair, nail, and urine specimens) of the cohort was stored for future studies. All steps of data collection and examinations are directly monitored by the quality control tea