The effect of preconditioning with high-intensity training on tissue levels of G-CSF, its receptor and C-kit after an acute myocardial infarction in male rats

Background: Exercise training is known as a practical way to increase cardioprotection against stress, and it seems that stem cell recruitment is one of its mechanisms. The purpose of the present study was to investigate the effect of preconditioning with High-intensity interval training (HIIT) on tissue levels of G-CSF, its receptor and C-Kit following acute myocardial infarction in male rats. Methods: Twenty Male Wistar rats were randomly divided into 4 groups of control, MI, HIIT, and HIIT+MI. Training groups performed 2 weeks of high intensity interval training in 4 sections. The first section consisted training in 3 days and 2 sessions in each day (4 � 2 min with 35-40 m/min and 3 � 2 min with 25-30 m/min between high intervals. The second part included 2 days of training (4 � 2 min with 40 to 45 m/min and 3 � 2 min with 28 to 32 m /min). The third part was performed in 3 days with one more repetition. The fourth section consisted 2 days of training and with one more repetition compared to section 3. For induction of myocardial infarction, subcutaneous injection of isoprenaline was used. CK, total CK, LDH, and troponin T were measured in serum and G-CSF, G-CSFR and C-Kit proteins were measured by the Western Blot method in the heart tissue. Results: The results of this study showed that enzymes of CK, total CK, LDH, troponin T had a significant increase in both MI and HIIT+MI groups compared to the other two groups (P < 0.001) and these indices in the MI group were significantly higher than the HIIT+MI group. Also, the results demonstrated that G-CSF, G-CSFR and C-Kit protein expression in the heart tissue significantly increased after MI. As well as, 2 weeks of HIIT training significantly increased G-CSF and C-kit in the training group compared to the control group, but the training caused that these proteins does not increase in HIIT+MI group as much as MI group. Conclusions: Along with other protective pathways, high intensity interval training can increase cardioprotection and decrease heart injuries through the increase in G-CSF, G-CSFR and C-kit level. © 2020 The Author(s)

A novel magnetic HS−-adsorptive nanocomposite photocatalyst (rGO/CoMn2O4-MgFe2O4) for hydrogen fuel production using H2S feed

Synthesis of low-cost, eco-friendly, semiconducting solar-energy materials with excellent photocatalytic activity [high surface area, good reactant adsorption, photon harnessing in the visible region, and low charge recombination] for application in pollutant conversion to hydrogen is of great importance from environmental remediation as well as green energy and fuel production perspectives. In the present work, a magnetic heterojunction of CoMn2O4/MgFe2O4 and reduced graphene oxide (rGO) was synthesized through a combined Hummers’/hydrothermal method. The obtained nanocomposite (rGO/CoMn2O4-MgFe2O4) was employed for photocatalytic conversion of H2S feed into hydrogen fuel. Adsorption studies in the feed solution proved a good capability for the photocatalyst to adsorb HS− reactant from the reaction medium. This effect was ascribed to the presence of the CoMn2O4 component, serving as a strong bisulfide adsorbent. VSM (vibrating sample magnetometry) analysis revealed that the magnetic property of the photocatalyst was due to the MgFe2O4 component. Photocatalytic investigations showed that the addition of rGO to the CoMn2O4/MgFe2O4 nanocomposite not only improves its reactant adsorption capacity, but also increases the photocatalyst surface area, enhances photon absorption, and suppresses the charge (e/h) recombination, which eventually boosts the photocatalyst activity to produce more hydrogen fuel (∼1.5 times)