Amplification results from field samples (Table S1) showing one or two peaks in the melting curve.

<p>Tm1: melting temperature of peak 1, Tm2: melting temperature of peak 2, SD: standard deviation, HRM %C: confidence percentage of HRM analysis, ND: not determined or not recognized, -: No secondary peak.</p

Blastn results of ten insects collected from the field.

<p>For each sample the identity with the standard species used for HRM identification is shown.</p

Source of the sample of the standard species used in the HRM analysis.

<p>-: sample not used.</p

Fourteen standard species included in the study and its respective Tm and HRM analysis.

<p>SD: standard deviation, CI: confidence interval, VC: variability correlation, %C: confidence percentage, %C SD: standard deviation of confidence percentage, Tm Genotype: Genotype according to Tm from all the replicas.</p

Samples from intestinal content and feces at different times after feeding.

<p>H: human, C: chicken, IC: intestinal content, F: feces, CG: chicken genotype, HG: human genotype, M: mixed feeding determined by two peaks in the melting curve, Tm1: melting temperature of peak 1, Tm2: melting temperature of peak 2, SD: standard deviation, HRM %C: confidence percentage HRM, %C SD standard deviation of confidence percentage, ND: not determined, -: No secondary peak.</p

Dissociation curve of 13 of the 14 genotypes included in the study.

<p>Dissociation curve of 13 of the 14 genotypes included in the study.</p

Tm distribution of 14 genotypes included in the study.

<p>Tm: melting temperature, Sp: species.</p