2 research outputs found
Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes
Abstract: In vitro tissue culture represents a useful technique for advancing Citrus breeding and propagation. Among in vitro regeneration systems, anther culture is commonly used to produce haploids and doubled haploids for a fast-track producing homozygous lines, in comparison with the traditional self-pollination approach, which involves several generations of selfing. In addition, anthers culture can produce somatic embryos that can also be used for clonal propagation. In this study, two thermal shocks were applied to the anthers of six Citrus genotypes (two clementine and four sweet oranges), just after they were put in culture. The response obtained was different depending on the genotype: both clementines, namely Hernandina and Corsica, produced homozygous and triploid regenerants (microspore-derived embryos), whereas all of the analyzed regenerants from sweet oranges, three cultivars of Tarocco and Moro, produced heterozygous and diploid regenerants similar to the parental genotypes (somatic embryos)
Effect of polyamines on in vitro anther culture of Citrus clementina Hort. ex Tan
The improvement of the induction rate
in Citrus anther culture is important for taking
practical advantage of the haploid potential in
breeding. The influence of polyamines on anther
culture of Citrus clementina, cv Nules, with
particular attention to the free, soluble and insoluble-
conjugated polyamine levels, has been investigated.
Putrescine, spermidine and putrescine plus
spermidine, were added to the standard induction
medium. Before culture, spermidine was the most
abundant among the free polyamines detected in
anthers. The exogenous supply of either putrescine
or spermidine, either independently or combined,
effected greater uptake and accumulation of
polyamines. The addition of 2 mM spermidine to
the medium stimulated gametic embryogenesis in
clementine Nules, whereas putrescine did not
influence embryo production. Regenerants were
mostly tri-haploids; a few doubled-haploids and no
haploid plants were obtained