Effect of 1,25-(OH)D(10M) on IL-6-related proliferation of confluent Caco-2/AQ cells

<p><b>Copyright information:</b></p><p>Taken from "Clone-specific expression, transcriptional regulation, and action of interleukin-6 in human colon carcinoma cells"</p><p>http://www.biomedcentral.com/1471-2407/8/13</p><p>BMC Cancer 2008;8():13-13.</p><p>Published online 18 Jan 2008</p><p>PMCID:PMC2257953.</p><p></p> Culture time was 72 h. Cell growth was assayed by [H]thymidine incorporation into cellular DNA (normalised to total protein). Data are expressed as means ± SD, = 4 – 7. Statistically significant differences from controls: *, < 0.05; **, < 0.01 (Student's -test)

Basal and stimulated expression of IL-6 mRNA and protein in human colon carcinoma cell clones

<p><b>Copyright information:</b></p><p>Taken from "Clone-specific expression, transcriptional regulation, and action of interleukin-6 in human colon carcinoma cells"</p><p>http://www.biomedcentral.com/1471-2407/8/13</p><p>BMC Cancer 2008;8():13-13.</p><p>Published online 18 Jan 2008</p><p>PMCID:PMC2257953.</p><p></p> Additions to the culture medium were: 10M indomethacin, 10M PGE, 10M 1,25-(OH)D, 10M 17β-E, 5 ng/ml IL-1β. Upper part: Representative RT-PCR amplifications of mRNA transcripts specific for (culture time 4 h). Expression of epithelial cell marker CK8 is shown for comparison. Lower part: IL-6 release into medium during 24 h culture period. Data are means ± SD, ≥ 4. Note that changes in IL-6 secretion by COGA-13 cells are given on a logarithmic scale. Statistically significant differences: *, < 0.05; **, < 0.01; ***, < 0.001 (Student's -test)

(A) Effect of hIL-6 on growth rate of confluent Caco-2/AQ, COGA-1A, and COGA-13 cells

<p><b>Copyright information:</b></p><p>Taken from "Clone-specific expression, transcriptional regulation, and action of interleukin-6 in human colon carcinoma cells"</p><p>http://www.biomedcentral.com/1471-2407/8/13</p><p>BMC Cancer 2008;8():13-13.</p><p>Published online 18 Jan 2008</p><p>PMCID:PMC2257953.</p><p></p> Cellular proliferation was evaluated from [H]thymidine incorporation into DNA after 72 h incubation with rhIL-6 (0–100 ng/ml). Data are means ± SD (= 4 – 16) and expressed as multiples of IL-6-free controls. Statistically significant differences from controls: **, < 0.01; ***, < 0.001 (Student's -test). (B) Time-course of mRNA expression in confluent Caco-2/AQ, COGA-1A, and COGA-13 clones during incubation with 100 ng/ml rhIL-6. Expression of the epithelial cell marker CK8 is shown for comparison. (C) Densitometric evaluation of expression of in relation to CK8 as shown in (B): basal expression ratios in zero time controls were set to 1