Estabelecimento de uma coleção de plasmídeos para transformação genética de eucalipto.

Editores técnicos: Marcílio José Thomazini, Elenice Fritzsons, Patrícia Raquel Silva, Guilherme Schnell e Schuhli, Denise Jeton Cardoso, Luziane Franciscon. EVINCI. Resumos

Integrated Diamond Optics for Single Photon Detection

Optical detection of single defect centers in the solid state is a key element of novel quantum technologies. This includes the generation of single photons and quantum information processing. Unfortunately the brightness of such atomic emitters is limited. Therefore we experimentally demonstrate a novel and simple approach that uses off-the-shelf optical elements. The key component is a solid immersion lens made of diamond, the host material for single color centers. We improve the excitation and detection of single emitters by one order of magnitude, as predicted by theory.Comment: 10 pages, 3 figure

Blood flow drives lumen formation by inverse membrane blebbing during angiogenesis in vivo

How vascular tubes build, maintain and adapt continuously perfused lumens to meet local metabolic needs remains poorly understood. Recent studies showed that blood flow itself plays a critical role in the remodelling of vascular networks, and suggested it is also required for the lumenization of new vascular connections. However, it is still unknown how haemodynamic forces contribute to the formation of new vascular lumens during blood vessel morphogenesis. Here we report that blood flow drives lumen expansion during sprouting angiogenesis in vivo by inducing spherical deformations of the apical membrane of endothelial cells, in a process that we have termed inverse blebbing. We show that endothelial cells react to these membrane intrusions by local and transient recruitment and contraction of actomyosin, and that this mechanism is required for single, unidirectional lumen expansion in angiogenic sprouts. Our work identifies inverse membrane blebbing as a cellular response to high external pressure. We show that in the case of blood vessels such membrane dynamics can drive local cell shape changes required for global tissue morphogenesis, shedding light on a pressure-driven mechanism of lumen formation in vertebrates

Efficient coupling of photons to a single molecule and the observation of its resonance fluorescence

Single dye molecules at cryogenic temperatures display many spectroscopic phenomena known from free atoms and are thus promising candidates for fundamental quantum optical studies. However, the existing techniques for the detection of single molecules have either sacrificed the information on the coherence of the excited state or have been inefficient. Here we show that these problems can be addressed by focusing the excitation light near to the absorption cross section of a molecule. Our detection scheme allows us to explore resonance fluorescence over 9 orders of magnitude of excitation intensity and to separate its coherent and incoherent parts. In the strong excitation regime, we demonstrate the first observation of the Mollow triplet from a single solid-state emitter. Under weak excitation we report the detection of a single molecule with an incident power as faint as 150 attoWatt, paving the way for studying nonlinear effects with only a few photons.Comment: 6 figure

Model of the Belousov-Zhabotinsky reaction

The article describes results of the modified model of the Belousov-Zhabotinsky reaction, which resembles rather well the limit set observed upon experimental performance of the reaction in the Petri dish. We discuss the concept of the ignition of circular waves and show that only the asymmetrical ignition leads to the formation of spiral structures. From the qualitative assumptions on the behavior of dynamic systems, we conclude that the Belousov-Zhabotinsky reaction likely forms a regular grid.Comment: 17 pages, 12 figure

Identificação e clonagem de promotores raiz-específicos de eucalipto.

A utilização de promotores tecido - específicos é uma das principais estratégias para direcionar a expres são de transgenes a determinados órgãos ou tipos celulares . Por isso, o objetivo deste trabalho foi a clonagem de promotores raiz - específicos , a partir de dados de RNA - Seq obtidos de bibliotecas de raiz, xilema e folha de eucalipto. Dois genes com padrão d e expressão preferencial em raiz foram inicialmente selecionados para isolamento da região promotora. Fragmentos de 1kb foram clonados em vetor binário pCAMBIA2301, fusionados ao gene repórter da beta - glucuronidase (GUS) , para transformação de Populus trem ula x Populus alba via Agrobacterium tumefaciens .Edição dos anais do 3º Simpósio de Bioquímica e Biotecnologia, 2013. Londrina

Genômica e biotecnologia aplicadas a adaptação a mudanças climáticas.

Mudanças climáticas globais e seus impactos na agricultura. Pesquisa e desenvolvimento de tecnologias transgênicas. Genômica funcional para descoberta de genes de valor agronômico. Bancos de dados genômicos. Genômica comparativa. Análise filogenética de famílias gênicas. Aplicações de tecnologias de sequenciamento de próxima geração. Seleção em larga escala de eventos transgênicos. A Unidade Mista de Pesquisa em Genômica Aplicada a Mudanças Climáticas (UMiP GenClima)

`In pursuit of the Nazi mind?' the deployment of psychoanalysis in the allied struggle against Germany

This paper discusses how psychoanalytic ideas were brought to bear in the Allied struggle against the Third Reich and explores some of the claims that were made about this endeavour. It shows how a variety of studies of Fascist psychopathology, centred on the concept of superego, were mobilized in military intelligence, post-war planning and policy recommendations for ‘denazification’. Freud's ideas were sometimes championed by particular army doctors and government planners; at other times they were combined with, or displaced by, competing, psychiatric and psychological forms of treatment and diverse studies of the Fascist ‘personality’. This is illustrated through a discussion of the treatment and interpretation of the deputy leader of the Nazi Party, Rudolf Hess, after his arrival in Britain in 1941