Induction of NY-ESO-1 by decitabine and 5-azacytidine.

<p>Photomicrograph showing the ability of decitabine and 5-azacytidine to induce of NY-ESO-1 <i>in vitro</i> in MCF-7 cells.</p

OS by NY-ESO-1 expression in TNBC.

<p>Kaplan-Meier curve showing OS differences in patients with TNBC stratified by NY-ESO-1 expression.</p

PFS by NY-ESO-1 expression in TNBC.

<p>Kaplan-Meier curve showing PFS differences in patients with TNBC stratified by NY-ESO-1 expression.</p

Patient characteristics.

1<p>These are not accurate reflections of the differences between TNBC and ER+/HER2- due to the sampling method.</p>2<p>LVI is lymphovascular invasion.</p

Extent of CD8 infiltration in triple negative tumors.

<p>This figure demonstrates the extent of CD8 infiltration in triple negative breast specimens.</p

NY-ESO-1 expression by IHC compared with a negative IgG control.

<p>Photomicrograph of NY-ESO-1 expression by immunohistochemistry side by side compared with a negative IgG control.</p

The relationship between NY-ESO-1 expression and degree of CD8 tumor infiltrating lymphocytes in TNBC.

<p>This figure shows the relationship between NY-ESO-1 expression and degree of CD8 tumor infiltrating lymphocytes in TNBC.</p

NY-ESO-1 expression frequency by IHC.

<p>NY-ESO-1 expression frequency by IHC.</p

Characteristics of NY-ESO-1 positive TNBC patients without NY-ESO-1 antibodies.

<p>Characteristics of NY-ESO-1 positive TNBC patients without NY-ESO-1 antibodies.</p

Global fitted (dotted line) SPR data of murine MX35 and Rebmab200 binding to immobilized synthetic NaPi2b epitope.

<p>MX35 (A) and Rebmab200 (B) were injected at concentrations ranging from 5 to 80 nM. After a 10 min association phase, the dissociation phase was followed for additional 10 min. Following double subtractive referencing, the curves were plotted using a 1∶1 Langmuir binding model, using Biacore T100 Evaluation Software. The solid line represents the experimental data and the dotted line the mathematical model for the binding of MX35 and Rebmab200 to the synthetic NaPi2b epitope.</p