Spectroscopic scan at 420 nm of a 150 bp PCR product (1 µg) containing two mismatches in the same amplicon after treatment with potassium permanganate (1 mM final concentration) at room temperature

<p><b>Copyright information:</b></p><p>Taken from "Detection of 100% of mutations in 124 individuals using a standard UV/Vis microplate reader: a novel concept for mutation scanning"</p><p>Nucleic Acids Research 2006;34(6):e45-e45.</p><p>Published online 22 Mar 2006</p><p>PMCID:PMC1409816.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> The top trace (red) shows the oxidation rate of the mismatched duplex, the middle traces (blue and green) show the oxidation rate of the matched duplex and the bottom trace (brown) shows the oxidation rate of the no-template control sample

Spectroscopic scan at 420 nm of 150 bp PCR products (1 µg), representing all four classes of mismatches, after treatment with potassium permanganate (1 mM final concentration) at room temperature ()

<p><b>Copyright information:</b></p><p>Taken from "Detection of 100% of mutations in 124 individuals using a standard UV/Vis microplate reader: a novel concept for mutation scanning"</p><p>Nucleic Acids Research 2006;34(6):e45-e45.</p><p>Published online 22 Mar 2006</p><p>PMCID:PMC1409816.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> G.T/A.C mismatch at position −57 (). C.T/A.G mismatch at position −43 (). C.C/G.G mismatch at position −76 (). A.A/T.T mismatch at position −2. In each scan, the top trace (red) shows the oxidation rate of the mismatched duplex, the middle trace (blue) shows the oxidation rate of the matched duplex and the bottom trace (brown) shows the oxidation rate of the no-template control sample

MOESM4 of HIV integration and the establishment of latency in CCL19-treated resting CD4+ T cells require activation of NF-ÎşB

Additional file 4: Figure S4. Integration site selection and gene activation in chemokine treated cells. A, Gene expression was determined by Illumina bead array in unactivated, CCL19-treated or PHA-IL2 activated CD4+ T cells after 6 or 72 h. The ratio of expression of genes at the sites of integration was determined in each in vitro condition. B, Expression of individual genes at the site of HIV integration in CCL19-treated resting CD4+ T cells (x-axis) compared to unactivated (y-axis; upper panel) or PHA-IL2 activated CD4+ T cells (y-axis; lower panel). C, The distance of integration sites to specific genomic elements including LINE, H4K20me3 and H4R3me following HIV infection of unactivated, CCL19-treated and PHA-IL2 activated CD4+ T cells, or CD4+ T cells from HIV-infected patients on cART or randomly selected sites. Log distance is shown as box plots (median and quartiles) with violin plot of the kernel distribution. The means are shown as a red horizontal line