Schematic representation of the distribution (%) of different KRAS mutations.

<p>Schematic representation of the distribution (%) of different KRAS mutations.</p

Pie charts representing the frequencies (%) of alterations of the examined genes in this cohort.

<p>Percentage of alterations for all the samples of the cohort (All NSCLC) and between the different histological types adenocarcinoma (AdCa), squamous cell carcinomas (Squamous), and other types (NOS and Other).</p

Histological Classification of 956 NSCLC samples

<p>A. Classification of Resection Samples B. Classification of Small Biopsies/cytology samples and C. Metastasis Samples according to IASLC/ATS/ERS 2011, WHO 2015.</p

Frequencies of alterations between different groups of adenocarcinomas (NL = Normal, MT = Mutant, AMPL = gene amplification).

<p>Frequencies of alterations between different groups of adenocarcinomas (NL = Normal, MT = Mutant, AMPL = gene amplification).</p

KRAS mutations distributed amongst different Adenocarcinoma groups.

<p>KRAS mutations distributed amongst different Adenocarcinoma groups.</p

EGFR mutations distributed amongst different Adenocarcinoma groups.

<p>EGFR mutations distributed amongst different Adenocarcinoma groups.</p

Clinicopathological and demographic characteristics of NSCLC patients.

<p>Clinicopathological and demographic characteristics of NSCLC patients.</p

Western blot analysis of H1X, H3K9me3 and H4K20me3 expression levels in astroglial cells SVG p12 and glioma cell line T98G.

<p>(A). Inhibition of SUV39H1 protein levels after treatment with 200 nM and 400 nM chaetocin by Western blot (B). MTT proliferation assays performed in glioma cells at 12 h and 24 h following treatment with chaetocin, indicating reduced cell proliferation following suppression of SUV39H1. All values represent means ± standard deviation (SD) of four parallel wells (C). Clonogenic assays of T98G cells performed following 12h treatment with chaetocin (200nM, 400nM). Colony counts (%) were evaluated relative to untreated control for T98G chaetocin-treated cells. Colony counts were done in triplicate of three independents experiments. Both colony formation and migration of T98G cells was reduced following chaetocin treatment compared to controls.</p

Characteristics of primary antibodies used in immunohistochemical (IHC) analysis.

<p>Characteristics of primary antibodies used in immunohistochemical (IHC) analysis.</p

Correlations among H1x, SETDB1, H3K4me3, H3K9me3 and SUV39H1 H-score in the entire cohort (Results of Spearman correlation coefficient). NS: not significant).

<p>Correlations among H1x, SETDB1, H3K4me3, H3K9me3 and SUV39H1 H-score in the entire cohort (Results of Spearman correlation coefficient). NS: not significant).</p