Characteristics of the race structure of <i>Leptosphaeria maculans</i> populations at different experimental sites in the UK, France and Germany.

<p>Characteristics of the race structure of <i>Leptosphaeria maculans</i> populations at different experimental sites in the UK, France and Germany.</p

Relationship between relative cultivar seed yield (<i>RY</i>, %) and relative cultivar phoma stem canker severity (<i>RS</i>, %).

<p>The relationship was fitted (broken short line) by the logistic equation () using the data from winter oilseed rape field experiments at 13 sites (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197752#pone.0197752.t001" target="_blank">Table 1</a>) in three growing seasons (2010/2011, 2011/2012, 2012/2013).</p

Relationships between cultivar phoma stem canker severity (<i>S</i>_<i>Ci</i>) and site mean phoma stem canker severity (<i>S</i>_<i>Sj</i>).

<p>The relationships between <i>S</i>_<i>Ci</i> and <i>S</i>_<i>Sj</i> in winter oilseed rape field experiments at 13 sites in three growing seasons (2010/11, 2011/12 and 2012/13). Analysis of position and parallelism showed five distinct groups, with three groups in Fig 3A: Drakkar (broken short line, <i>S</i>_<i>C</i> = 2.53+0.96<i>S</i>_<i>S</i>), Capitol and Bilbao (broken long line, <i>S</i>_<i>C</i> = 0.22+1.14<i>S</i>_<i>S</i>), NK Grandia (solid line, <i>S</i>_<i>C</i> = 0.14+0.88<i>S</i>_<i>S</i>) and with two groups in Fig 3B: Adriana and DK Cabernet (broken long-short line, <i>S</i>_<i>C</i> = -0.50+1.05<i>S</i>_<i>S</i>), Ex-Astrid, Roxet and Excel (dash-dot line, <i>S</i>_<i>C</i> = -0.70+0.92<i>S</i>_<i>S</i>).</p

Comparison of phoma stem canker severity and seed yield between different oilseed rape cultivars grown in winter oilseed rape field experiments at 13 sites over three growing seasons (2010/2011, 2011/2012, 2012/2013).

<p>Comparison of phoma stem canker severity and seed yield between different oilseed rape cultivars grown in winter oilseed rape field experiments at 13 sites over three growing seasons (2010/2011, 2011/2012, 2012/2013).</p

Oilseed rape (<i>Brassica napus</i>) cultivars/lines and <i>Leptosphaeria maculans</i> isolates used in the controlled environment experiments to confirm the presence of <i>Rlm</i> genes or quantitative resistant (QR).

<p>Oilseed rape (<i>Brassica napus</i>) cultivars/lines and <i>Leptosphaeria maculans</i> isolates used in the controlled environment experiments to confirm the presence of <i>Rlm</i> genes or quantitative resistant (QR).</p

Distribution of relative seed yield.

<p>The distribution of relative seed yield for each cultivar from field experiments at 13 sites in three growing seasons (2010/11, 2011/12 and 2012/13). Each box-plot shows the mean (broken line) and median (solid line) relative seed yield. The lower and upper boundaries of the boxes indicate relative seed yield for the 25^th and 75^th percentiles, while whisker bars below and above each box indicate relative seed yield for the 5^th and 95^th percentiles. The black dots below and above each box-plot represent the minimum and maximum seed yields, respectively.</p

The latitude and longitude for field experiment sites and the associated weather stations over three growing seasons (2010/11, 2011/12 and 2012/13).

<p>The latitude and longitude for field experiment sites and the associated weather stations over three growing seasons (2010/11, 2011/12 and 2012/13).</p

Relationship between individual cultivar seed yield (<i>Y</i>_<i>Ci</i>) and site mean seed yield (<i>Y</i>_<i>Sj</i>).

<p>The relationship between <i>Y</i>_<i>Ci</i> and <i>Y</i>_<i>Sj</i> in winter oilseed rape field experiments at 13 sites in three growing seasons (2010/11, 2011/12 and 2012/13). Analysis of position and parallelism showed five distinct groups, with three groups in Fig 5A: Excel (broken short line, <i>Y</i>_<i>C</i> = 0.03+1.18<i>Y</i>_<i>S</i>), NK Grandia and Adriana (solid line, <i>Y</i>_<i>C</i> = -0.01+1.11<i>Y</i>_<i>S</i>), Drakkar (dash-dot line, <i>Y</i>_<i>C</i> = -0.39 + 0.58<i>Y</i>_<i>S</i>) and with two groups in Fig 5B: Ex-Astrid, DK Cabernet and Bilbao (broken long line, <i>Y</i>_<i>C</i> = 0.40+1.02<i>Y</i>_<i>S</i>), Roxet and Capitol (broken long-short line, <i>Y</i>_<i>C</i> = -0.29+0.99<i>Y</i>_<i>S</i>).</p

Phoma stem canker severity in controlled environment experiment.

<p>Cultivar difference in the severity of phoma stem canker scored on a 0–6 scale (A) and the internal length of phoma stem canker symptoms (B) that had developed at 20°C by 49 days after inoculation of leaf petioles of different cultivars/lines with conidial suspensions of <i>Leptosphaeria maculans</i> isolates. For details of isolates, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197752#pone.0197752.t002" target="_blank">Table 2</a>. Error bars are standard errors of the difference. Multiple comparisons were done using the least significance difference at significance level <i>P</i>≤0.05. The values for phoma stem canker severity or the internal length of phoma stem canker symptoms that had different letters differed significantly at <i>P</i>≤0.05.</p

Distribution of relative phoma stem canker severity.

<p>The distribution of relative phoma stem canker severity for each cultivar from winter oilseed rape field experiments at 13 sites in three growing seasons (2010/11, 2011/12 and 2012/13). Each box-plot shows the mean (broken line) and median (solid line) percentage relative phoma stem canker severity. The lower and upper boundaries of the boxes indicate relative phoma stem canker severity for the 25^th and 75^th percentiles, while whisker bars below and above each box indicate relative phoma stem canker severity for the 5^th and 95^th percentiles. The black dots below and above each box-plot represent the minimum and maximum relative phoma stem canker severities, respectively.</p