Location map of EBHSV positive <i>Lepus europaeus</i> individuals.

<p>Location map of the 65 <i>Lepus europaeus</i> individuals sampled throughout Denmark that were affected by the virus of the European Brown Hare Syndrome (EBHSV).</p

Groups of Danish brown hares individuals according to the presence of EBHSV and the variation in exon 2 DQA gene.

<p>Numbers (N) and percentages of Danish brown hares assessed for the four groups defined in this study: EBHSV negative (EBHSV⁻), EBHSV positive (EBHSV⁺), susceptible (EBHSV⁺ Dead), resistant (EBHSV⁺ not Dead).</p

Environmental variables used in the analysis.

<p>Environmental variables used in the analysis.</p

Map of Greece showing potential geographic distribution of WNV.

<p>Map of Greece showing potential geographic distribution of WNV, predicted by GIS and MD based on the attributes of the major clusters of reported human cases of 2011 and seropositive wild birds (low altitude, small distance from water). Black dots indicate reported human cases in 2012. Black circles indicate suggested high-risk areas for WNV further dispersion in following years.</p

Map of Greece showing WNV laboratory-confirmed human cases and seropositive resident wild birds, 2010–2012.

<p>Map of Greece showing the distribution of WNV laboratory-confirmed human cases and seropositive resident wild bird samples for the 2010–2012 period. Red, yellow and green dots indicate human cases reported in 2010, 2011 and 2012 respectively. Black dots indicate seropositive resident wild birds detected during the same period. Text boxes refer to available avian samples (resident and migratory) per each region.</p

Phylogenetic tree of the EBHSV isolates.

<p>Phylogenetic tree resulting from the Bayesian analysis, clustering the seven Danish isolates identified in this study combined with sequences available from the EMBL database. The topology of the clusters was similar for the NJ tree. Numbers on branches at the internodes of the clusters correspond to posterior probabilities from the Bayesian analysis. At the end of the branches the designation and origin of the EBHS viruses studied. Probabilities below 50% are omitted.</p

Percentages indicating the occurrence of the homogygous and heterozygous <i>DQA</i> genotypes.

<p>Percentages indicating the occurrence of the homogygous and heterozygous <i>DQA</i> genotypes in the four groups of <i>Lepus europaeus</i> sampled in Denmark. Bars indicate the occurrence of each genotype in non affected or affected animals. Pies above bars indicate the occurrence of each genotype among the affected <i>L. europeaus</i> individuals in susceptible animals found dead with lesions typical to the EBHS in various organs or resistant not dead animals.</p

Descriptive statistics of positive human cases 2010–2011.

<p>*<i>Statistically significant reduction in 2011 (5 years) (p-value = 0.024<0.05).</i></p>+<p><i>Independence between 2010 and 2011 (p-value = 0.000<0.001).</i></p

DQA alleles (residues 8–76) of <i>Lepus europaeus.</i>

<p>DQA alleles (residues 8–76) of <i>Lepus europaeus.</i> Polymorphic residues are in bold. Residues contributing to the formation of binding pockets P1, P6 and P9 are indicated. Shaded residues are putative TCR contacts. Crosses indicate residues with hydrogen bonds to the peptide. The boxed area determines αβ pairing.</p

Available avian samples: species, migratory status and number of samples per region.

<p>Available avian samples: species, migratory status and number of samples per region.</p