Improved Parameters and New Lensed Features for Q0957+561 from WFPC2 Imaging

New HST WFPC2 observations of the lensed double QSO 0957+561 will allow improved constraints on the lens mass distribution and hence will improve the derived value of H$_0$. We first present improved optical positions and photometry for the known components of this lens. The optical separation between the A and B quasar images agrees with VLBI data at the 10 mas level, and the optical center of the primary lensing galaxy G1 coincides with the VLBI source G' to within 10 mas. The best previous model for this lens (Grogin and Narayan 1996) is excluded by these data and must be reevaluated. Several new resolved features are found within 10\arcsec of G1, including an apparent fold arc with two bright knots. Several other small galaxies are detected, including two which may be multiple images of each other. We present positions and crude photometry of these objects.Comment: 7 pages including 2 postscript figures, LaTeX, emulateapj style. Also available at http://www.astro.lsa.umich.edu:80/users/philf/www/papers/list.htm

Stock Assessment of Queensland East Coast black jewfish (Protonibea diacanthus), Australia, with data to December 2021

Black jewfish (Protonibea diacanthus) are caught on the east coast of Queensland by commercial, recreational, charter and Indigenous fishers. The fishery is focused around Central Queensland, and has recently experienced a large shift in commercial effort and gear types. The species was historically considered a byproduct species within the inshore net fishery, but has now become a targeted line caught species. In Australia, black jewfish are found from Exmouth Gulf in Western Australia, north and east across Northern Australia, to the east coast of Queensland. Research suggests that stocks cover hundreds of kilometres. This is the first stock assessment of the Queensland East Coast stock. The stock assessment was conducted on calendar years and included input data through to December 2021. Eight model scenarios were run, covering different combinations of recreational dead catch and population steepness (productivity parameter). Base case (preferred) scenario results suggested that biomass declined gradually from the 1940s to the 1980s and has increased slightly since then. The spawning stock level at the beginning of 2022 for base-case scenarios was estimated to be between 56% and 99% with a median estimate of 79%. The estimates from other scenarios ranged from 80% to 92%. Despite high uncertainty around the exact level of biomass, the model outputs indicate that the biomass is probably at or above the target reference point of 60% unfished biomass

Schwarzschild black hole lensing

We study strong gravitational lensing due to a Schwarzschild black hole. Apart from the primary and the secondary images we find a sequence of images on both sides of the optic axis; we call them {\em relativistic images}. These images are formed due to large bending of light near r = 3M (the closest distance of approach r_o is greater than 3M). The sources of the entire universe are mapped in the vicinity of the black hole by these images. For the case of the Galactic supermassive ``black hole'' they are formed at about 17 microarcseconds from the optic axis. The relativistic images are not resolved among themselves, but they are resolved from the primary and secondary images. However the relativistic images are very much demagnified unless the observer, lens and source are very highly aligned. Due to this and some other difficulties the observation of these images does not seem to be feasible in near future. However, it would be a great success of the general theory of relativity in a strong gravitational field if they ever were observed and it would also give an upper bound, r_o = 3.21 M, to the compactness of the lens, which would support the black hole interpretation of the lensing object.Comment: RevTex, 5 eps files are included, observational difficulties are discussed and there are some changes in presentatio

Simultaneous X-ray and UV spectroscopy of the Seyfert 1 galaxy NGC 5548.II. Physical conditions in the X-ray absorber

We present the results from a 500 ks Chandra observation of the Seyfert 1 galaxy NGC 5548. We detect broadened emission lines of O VII and C VI in the spectra, similar to those observed in the optical and UV bands. The source was continuously variable, with a 30 % increase in luminosity in the second half of the observation. No variability in the warm absorber was detected between the spectra from the first 170 ks and the second part of the observation. The velocity structure of the X-ray absorber is consistent with the velocity structure measured simultaneously in the ultraviolet spectra. We find that the highest velocity outflow component, at -1040 km/s, becomes increasingly important for higher ionization parameters. This velocity component spans at least three orders of magnitude in ionization parameter, producing both highly ionized X-ray absorption lines (Mg XII, Si XIV) as well as UV absorption lines. A similar conclusion is very probable for the other four velocity components. Based upon our observations, we argue that the warm absorber probably does not manifest itself in the form of photoionized clumps in pressure equilibrium with a surrounding wind. Instead, a model with a continuous distribution of column density versus ionization parameter gives an excellent fit to our data. From the shape of this distribution and the assumption that the mass loss through the wind should be smaller than the accretion rate onto the black hole, we derive upper limits to the solid angle as small as 10^{-4} sr. From this we argue that the outflow occurs in density-stratified streamers. The density stratification across the stream then produces the wide range of ionization parameter observed in this source. Abridged.Comment: 21 pages, 12 figures accepted for publication in A&

Genomic mapping of phosphorothioates reveals partial modification of short consensus sequences

Bacterial phosphorothioate (PT) DNA modifications are incorporated by Dnd proteins A-E and often function with DndF-H as a restriction-modification (R-M) system, as in Escherichia coli B7A. However, bacteria such as Vibrio cyclitrophicus FF75 lack dndF-H, which points to other PT functions. Here we report two novel, orthogonal technologies to map PTs across the genomes of B7A and FF75 with >90% agreement: single molecule, real-time sequencing and deep sequencing of iodine-induced cleavage at PT (ICDS). In B7A, we detect PT on both strands of G[subscript ps]AAC/G[subscript ps]TTC motifs, but with only 12% of 40,701 possible sites modified. In contrast, PT in FF75 occurs as a single-strand modification at C[subscript ps]CA, again with only 14% of 160,541 sites modified. Single-molecule analysis indicates that modification could be partial at any particular genomic site even with active restriction by DndF-H, with direct interaction of modification proteins with GAAC/GTTC sites demonstrated with oligonucleotides. These results point to highly unusual target selection by PT-modification proteins and rule out known R-M mechanisms.National Natural Science Foundation (China)Ministry of Science and Technology of the People's Republic of China (973 and 863 Programs)Shanghai Municipal Council of Science and Technology. Shanghai Pujiang ProgramNational Science Foundation (U.S.) (CHE-1019990)National Institute of Environmental Health Sciences (ES002109)Singapore. National Research Foundation (Singapore-MIT Alliance for Research and Technology

Synergy Between Intercellular Communication and Intracellular Ca2+ Handling in Arrhythmogenesis

Calcium is the primary signalling component of excitation-contraction coupling, the process linking electrical excitability of cardiac muscle cells to coordinated contraction of the heart. Understanding Ca2þ handling processes at the cellular level and the role of intercellular communication in the emergence of multicellular synchronization are key aspects in the study of arrhythmias. To probe these mechanisms, we have simulated cellular interactions on large scale arrays that mimic cardiac tissue, and where individual cells are represented by a mathematical model of intracellular Ca2þ dynamics. Theoretical predictions successfully reproduced experimental findings and provide novel insights on the action of two pharmacological agents (ionomycin and verapamil) that modulate Ca2þ signalling pathways via distinct mechanisms. Computational results have demonstrated how transitions between local synchronisation events and large scale wave formation are affected by these agents. Entrainment phenomena are shown to be linked to both ntracellular Ca2þ and coupling-specific dynamics in a synergistic manner. The intrinsic variability of the cellular matrix is also shown to affect emergent patterns of rhythmicity, providing insights into the origins of arrhythmogenic Ca2þ perturbations in cardiac tissue in situ

Decolonizing Science and Science Education in a Postcolonial Space (Trinidad, a Developing Caribbean Nation, Illustrates)

The article addresses how remnant or transformed colonialist structures continue to shape science and science education, and how that impact might be mitigated within a postcolonial environment in favor of the development of the particular community being addressed. Though cognizant of, and resistant to, the ongoing colonial impact globally and nationally (and any attempts at subjugation, imperialism, and marginalization), this article is not about anticolonial science. Indeed, it is realized that the postcolonial state of science and science education is not simply defined, and may exist as a mix of the scientific practices of the colonizer and the colonized. The discussion occurs through a generic postcolonial lens and is organized into two main sections. First, the discussion of the postcolonial lens is eased through a consideration of globalization which is held here as the new colonialism. The article then uses this lens to interrogate conceptions of science and science education, and to suggest that the mainstream, standard account of what science is seems to represent a globalized- or arguably a Western, modern, secular-conception of science. This standard account of science can act as a gatekeeper to the indigenous ways of being, knowing, and doing of postcolonial populations. The article goes on to suggest that as a postcolonial response, decolonizing science and science education might be possible through practices that are primarily contextually respectful and responsive. That is, localization is suggested as one possible antidote to the deleterious effects of globalization. Trinidad, a postcolonial developing Caribbean nation, is used as illustration

Modelling molecular interaction pathways using a two-stage identification algorithm

In systems biology, molecular interactions are typically modelled using white-box methods, usually based on mass action kinetics. Unfortunately, problems with dimensionality can arise when the number of molecular species in the system is very large, which makes the system modelling and behavior simulation extremely difficult or computationally too expensive. As an alternative, this paper investigates the identification of two molecular interaction pathways using a black-box approach. This type of method creates a simple linear-in-the-parameters model using regression of data, where the output of the model at any time is a function of previous system states of interest. One of the main objectives in building black-box models is to produce an optimal sparse nonlinear one to effectively represent the system behavior. In this paper, it is achieved by applying an efficient iterative approach, where the terms in the regression model are selected and refined using a forward and backward subset selection algorithm. The method is applied to model identification for the MAPK signal transduction pathway and the Brusselator using noisy data of different sizes. Simulation results confirm the efficacy of the black-box modelling method which offers an alternative to the computationally expensive conventional approach