A method for large-scale identification of protein arginine methylation.

The lack of methods for proteome-scale detection of arginine methylation restricts our knowledge of its relevance in physiological and pathological processes. Here we show that most tryptic peptides containing methylated arginine(s) are highly basic and hydrophilic. Consequently, they could be considerably enriched from total cell extracts by simple protocols using either one of strong cation exchange chromatography, isoelectric focusing, or hydrophilic interaction liquid chromatography, the latter being by far the most effective of all. These methods, coupled with heavy methyl-stable isotope labeling by amino acids in cell culture and mass spectrometry, enabled in T cells the identification of 249 arginine methylation sites in 131 proteins, including 190 new sites and 93 proteins not previously known to be arginine methylated. By extending considerably the number of known arginine methylation sites, our data reveal a novel proline-rich consensus motif and identify for the first time arginine methylation in proteins involved in cytoskeleton rearrangement at the immunological synapse and in endosomal trafficking

Is the timing of spawning in sparid fishes a response to sea temperature regimes?

Published spawning seasons of sparid fish were investigated to determine if there were consistent patterns that could be related to large-scale physical variability, and whether these relationships were species-specific or characteristic of higher taxonomic groupings. For individual species, genera and the family Sparidae as a whole, there was a consistent pattern; spawning at lower latitudes was concentrated close to the month of lowest sea surface temperature, while spawning at higher latitudes was more variable with greater deviations from the month of minimum sea surface temperature. The distribution of sparids may be limited by a lack of tolerance of one or more early life-history stage to high water temperatures, so targeting spawning to the coolest part of the year could be a tactic allowing maximum penetration into warmer waters. Such a link between the physiology of early life-history stages and timing of spawning could have direct consequences for patterns of distributions over a number of taxonomic scales. If there are similar constraints on the reproduction of other species, even minor increases in water temperature due to global warming that may be within the tolerance of adults, may impose constraints on the timing of spawning, with flow-on effects for both species and whole ecosystems

Kreislaufstillstand in besonderen Situationen

Truhlář A, Deakin CD, Soar J, et al. Kreislaufstillstand in besonderen Situationen. Notfall + Rettungsmedizin. 2015;18(8):833-903