Profiling the mouse brain endothelial transcriptome in health and disease models reveals a core blood-brain barrier dysfunction module.

Blood vessels in the CNS form a specialized and critical structure, the blood-brain barrier (BBB). We present a resource to understand the molecular mechanisms that regulate BBB function in health and dysfunction during disease. Using endothelial cell enrichment and RNA sequencing, we analyzed the gene expression of endothelial cells in mice, comparing brain endothelial cells with peripheral endothelial cells. We also assessed the regulation of CNS endothelial gene expression in models of stroke, multiple sclerosis, traumatic brain injury and seizure, each having profound BBB disruption. We found that although each is caused by a distinct trigger, they exhibit strikingly similar endothelial gene expression changes during BBB disruption, comprising a core BBB dysfunction module that shifts the CNS endothelial cells into a peripheral endothelial cell-like state. The identification of a common pathway for BBB dysfunction suggests that targeting therapeutic agents to limit it may be effective across multiple neurological disorders

Investigation of the feasibility of relative 3D dosimetry in the Radiologic Physics Center Head and Neck IMRT phantom using Presage/optical-CT

This study presents the application of the Presage∕optical-CT 3D dosimetry system for relative dosimetry in the Radiologic Physics Center (RPC) Head and Neck (H&N) IMRT phantom. Performance of the system was evaluated by comparison with the “gold-standard” RPC credentialing test. A modified Presage cylindrical insert was created that extended the capability of the RPC H&N phantom to 3D dosimetry. The RPC phantom was taken through the entire treatment planning procedure with both the standard RPC insert and the modified Presage insert. An IMRT plan was created to match the desired dose constraints of the credentialing test. This plan was delivered twice to the RPC phantom: first containing the standard insert, and then again containing the Presage insert. After irradiation, the standard insert was sent for routine credentialing analysis; including point dose measurements (TLD) and planar Gafchromic® EBT film measurement. The 3D dose distribution from Presage was read out at Duke using the OCTOPUS™ 5X optical-CT scanner. The Presage distribution was compared with gold-standard EBT measurement (determined by the RPC) and the calculated Eclipse distribution. The agreement between the normalized EBT, Presage, and Eclipse distributions, in the central axial plane was evaluated using profiles and gamma-map comparisons (4% dose difference and 3 mm distance to agreement). Profiles showed good agreement between EBT, Presage, and Eclipse distributions. 2D gamma-map comparisons between all three modalities showed at least 98% pass rate. The excellent agreement between Presage and EBT in the central plane established Presage as a standard against which to evaluate the accuracy of the 3D calculated Eclipse distribution. A gamma comparison between normalized Presage and Eclipse 3D distributions gave an overall pass rate of ∼94%. In conclusion, the Presage∕optical-CT system was found to be feasible for relative 3D dosimetry in the RPC IMRT H&N phantom. The potential to extend the RPC IMRT credentialing procedure to 3D may be feasible provided accurate calibration to dose (Gy) and robustness to shipping stress are demonstrated

PARAID: A Gear-shifting Power-aware RAID

Reducing power consumption for server-class computers is important, since increased energy usage causes more heat dissipation, greater cooling requirements, reduced computational density, and higher operating costs. For a typical data center, storage accounts for 27% of energy consumption. Conventional server-class RAIDs cannot easily reduce power because loads are balanced to use all disks, even for light loads. We have built the power-aware RAID (PARAID), which reduces energy use of commodity server-class disks without specialized hardware. PARAID uses a skewed striping pattern to adapt to the system load by varying the number of powered disks. By spinning disks down during light loads, PARAID can reduce power consumption, while still meeting performance demands, by matching the number of powered disks to the system load. Reliability is achieved by limiting disk power cycles and using different RAID encoding schemes. Based on our five-disk prototype, PARAID uses up to 34% less power than conventional RAIDs while achieving similar performance and reliability