Two subforms of eukaryotic topoisomerase I Purification and structure-function relationships

AbstractA new method for isolation of eukaryotic topoisomerase 1 from calf thymus and from Jurkat-1 cells using HPLC has been developed. The method allows quantitative purification of high molecular weight topo I and of two low molecular weight fractions differing by their isoelectric points. It has been suggested that these fractions be characterized as two subforms of the enzyme possessing structural and functional differences. The differences in their specific activities, sensitivity to camptothecin and in their proteolytic digestion maps have been demonstrated for the two enzymes

An Effect of Co–W Barrier Sublayer on the Functional Characteristics of Au–Ru Contact Coatings

Funding Information: Funding: This work was supported by the Ministry of Education and Science of the Russian Federation in the frame of the state assignment FSSN-2020-0003. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.The performance characteristics (microhardness, porosity, roughness, and transient resistance) of Au–Ru coatings with and without the Co–W alloy barrier sublayer electrodeposited on the surface of contact blades of commercially produced reed switches were investigated. It was found that the barrier sublayer reduces the average roughness of the coatings without significant change in their porosity. The Au–Ru coatings without sublayer exhibited a greater variation in the transient resistance during an increase in the pressing force. The service time of reed switches with the barrier sublayer increased in both testing modes: in low-power (50 mV, 5 µA, 50 Hz) by more than 4 × 106 switching cycles, and in mean-power (12 V, 0.25 A, 50 Hz) by more than 1.8 × 106 switching cycles.publishersversionpublishe

Switching Properties of the Contact Coatings Based on Refractory Metals Alloys

Switching the properties of the Ag contact groups of an electromagnetic relay with the electrodeposited Ni-W and Co-W alloy coatings was studied. It is shown that these coatings are able to stabilize the spread of contact resistance within the entire test interval and to increase the service life of Ag contact groups from 2.3 × 106 to 4.5 × 106 commutation cycles. According to our research, the service life of coated contact groups is mainly determined by a change in the surface morphology caused by the electrical erosive transfer of the contact materials and to a lesser extent by the formation of poorly conductive adsorbed hydrocarbon films

Electrochemical Deposition of Ni–W Crack-Free Coatings

The main features of electrochemical deposition of coatings based on Ni–W binary alloy in the pulse current mode using pyrophosphate electrolytes were studied. Two electrolytes with a pH of 8.7 and 9.5 were used. The deposition was carried out with the current density varying in the range of 0.01–0.1 A·cm−2, and the duty cycle (the relative pulse duration) was changed within the range 20–100%. The surface morphology and elemental and phase composition of the coatings were studied by scanning electron microscopy, energy-dispersive X-ray microanalysis and X-ray diffractometry. The experimental conditions allowing us to achieve the maximum Faradaic efficiency and W content in the coatings were determined. It was found that the pulse current mode enabled the fabrication of crack-free coatings with a thickness greater than 6 μm