Univariate analysis of various variables.

*<p>Statistical significant.</p

Immunohistochemical analysis of KIF26B in breast cancer.

<p>Normal breast contains rare KIF26B-positive cells <b>(A)</b>, whereas breast cancer tissues show weak <b>(B)</b>, moderate <b>(C)</b>, and strong <b>(D)</b> staining of KIF26B. Numerical scores: A = 0, B = 2, C = 6, D = 12.</p

Relationship between clinicopathologic factors and KIF26B expression.

*<p>Statistical significant.</p

Kaplan-Meier analysis of cancer-specific survival.

<p>KIF26B protein levels showed prognostic role in overall survival <b>(A)</b> and disease-free survival <b>(B)</b>.</p

Supplementary document for Analog multiplexing of laser clock and computational photon counting for fast fluorescence lifetime imaging microscopy - 6881315.pdf

Supplementary document containing Notes S1-S2, Fig. S1-S10, and Tables S1-S

Additional file 1 of Circ-CSNK1G1 promotes cell proliferation, migration, invasion and glycolysis metabolism during triple-negative breast cancer progression by modulating the miR-28-5p/LDHA pathway

Additional file 1

KIF26B mRNA and protein expression in breast cancer.

<p><b>A,</b> KIF26B mRNA was upregulation in 27 of 30 breast cancer cases. The relative KIF26B mRNA expression was indicated by histogram (Tumor, primary breast cancer tissues; Normal, the paired adjacent normal breast tissues). <b>B,</b> The KIF26B mRNA levels was significantly upregulation in breast cancer as determined by matched paired Students' <i>t</i> test. <b>C,</b> KIF26B protein expression was upregulation in 26 of 30 breast cancer cases by western blot. β-actin was used as the loading control.</p

Image_1_Increased plasma genistein after bariatric surgery could promote remission of NAFLD in patients with obesity.tif

BackgroundBariatric surgery is associated with a positive effect on the progress of non-alcoholic associated fatty liver disease (NAFLD). Although weight loss is the obvious mechanism, there are also weight-independent mechanisms.MethodsWe collected blood samples from 5 patients with obesity before and 3 months after surgery and performed an LC-MS-based untargeted metabolomics test to detect potential systemic changes. We also constructed sleeve gastrectomy (SG) mice models. The plasma, liver and intestine samples were collected and analyzed by qPCR, ELISA and HPLC. Cohousing experiments and feces transplantation experiments were performed on mice to study the effect of gut microbiota. Genistein administration experiments were used to study the in vivo function of the metabolites.ResultsPlasma genistein (GE) was identified to be elevated after surgery. Both clinical data and rodent models suggested that plasma GE is negatively related to the degree of NAFLD. We fed diet-induced obese (DIO) mice with GE, and we found that there was significant remission of NAFLD. Both in vivo and in vitro experiments showed that GE could restrict the inflammation state in the liver and thus relieve NAFLD. Finally, we used co-housing experiments to alter the gut microbiota in mice, and it was identified that sleeve gastrectomy (SG) mice had a special gut microbiota phenotype, which could result in higher plasma GE levels. By feces transplantation experiment (FMT), we found that only feces from the SG mice (and not from other lean mice) could induce higher plasma GE levels.ConclusionOur studies showed that SG but not calorie restriction could induce higher plasma GE levels by altering the gut microbiota. This change could promote NAFLD remission. Our study provides new insights into the systemic effects of bariatric surgery. Bariatric surgery could affect remote organs via altered metabolites from the gut microbiota. Our study also identified that additional supplement of GE after surgery could be a therapy for NAFLD.</p

Two-Dimensional Organic–Inorganic Hybrid Perovskite Photonic Films

Organic–inorganic hybrid perovskites have created enormous expectations for low-cost and high-performance optoelectronic devices. In prospect, future advancements may derive from reaping novel electrical and optical properties beyond pristine perovskites through microscopic structure design and engineering. Herein, we report the successful preparation of two-dimensional inverse-opal perovskite (IOP) photonic films, featuring unique nanostructures and vivid colors. Further compositional and structural managements promise optical property and energy level tunability of the IOP films. They are further functionalized in solar cells, resulting in colorful devices with respectable power conversion efficiency. Such concept has not been previously applied for perovskite-based solar cells, which could open a route for more versatile optoelectronic devices

Image_1_GLS as a diagnostic biomarker in breast cancer: in-silico, in-situ, and in-vitro insights.jpeg

BackgroundRecently, a novel programmed cell death mechanism, Cuproptosis, has been discovered and found to play an important role in the development and progression of diverse tumors. In the present study, we comprehensively investigated the core gene of this mechanism, GLS, in breast cancer.Materials and methodsBulk RNA sequencing data were curated from the TCGA repository to investigate the aberrant expression of GLS over diverse cancer types. Then, we examined its efficacy as a diagnostic biomarker in breast cancer by Area Under Curve (AUC) of the Receiver Operative Characteristic (ROC) curve. Furthermore, by applying siRNA technique, we knocked down the GLS expression level in cancerous cell lines, measuring the corresponding effects on cell proliferation and metastasis. Afterward, we explored the potential implications of GLS expression in the tumor immune microenvironment quantitatively by using several R packages and algorithms, including ESTIMATE, CIBERSORT, etc.ResultsPan-cancer analysis suggested that GLS was aberrantly over-expressed in many cancer types, with breast cancer being typical. More in-depth analyses revealed the expression of GLS exerted a high ROC-AUC value in breast cancer diagnosis. Through the knock-down of GLS expression, it was found that GLS expression was strongly relevant to the growth and metastasis of tumor. Furthermore, it was also found to be correlated with the immune tumor microenvironment.ConclusionWe highlighted that GLS expression might be applicable as a diagnostic biomarker in breast cancer and possess significant implications in the growth and metastasis of tumor and the immune tumor microenvironment, sharing new insights into ontological and personalized medicine.</p