Telomere length dynamics and the evolution of cancer genome architecture

Telomeres are progressively eroded during repeated rounds of cell division due to the end replication problem but also undergo additional more substantial stochastic shortening events. In most cases, shortened telomeres induce a cell-cycle arrest or trigger apoptosis, although for those cells that bypass such signals during tumour progression, a critical length threshold is reached at which telomere dysfunction may ensue. Dysfunction of the telomere nucleoprotein complex can expose free chromosome ends to the DNA double-strand break (DSB) repair machinery, leading to telomere fusion with both telomeric and non-telomeric loci. The consequences of telomere fusions in promoting genome instability have long been appreciated through the breakage–fusion–bridge (BFB) cycle mechanism, although recent studies using high-throughput sequencing technologies have uncovered evidence of involvement in a wider spectrum of genomic rearrangements including chromothripsis. A critical step in cancer progression is the transition of a clone to immortality, through the stabilisation of the telomere repeat array. This can be achieved via the reactivation of telomerase, or the induction of the alternative lengthening of telomeres (ALT) pathway. Whilst telomere dysfunction may promote genome instability and tumour progression, by limiting the replicative potential of a cell and enforcing senescence, telomere shortening can act as a tumour suppressor mechanism. However, the burden of senescent cells has also been implicated as a driver of ageing and age-related pathology, and in the promotion of cancer through inflammatory signalling. Considering the critical role of telomere length in governing cancer biology, we review questions related to the prognostic value of studying the dynamics of telomere shortening and fusion, and discuss mechanisms and consequences of telomere-induced genome rearrangements

Compuesto etileno acetato de vinilo (EVA) reforzado con neumáticos fuera de uso (GTR) propiedades dieléctricas, mecánicas y térmicas

La masiva producción de neumáticos, así como su posterioralmacenamiento después de su utilización constituye un grave problema medioambiental al que se pretende dar salida con diversas soluciones. Una de ellas pasa por mezclar estos neumáticos reutilizados (Ground Tire Rubber GTR) con diversos polímeros termoplásticos, siendo esta opción la analizada en el presente trabajo, el cual tiene como objetivo obtener materiales aislantes adecuados para la industria eléctrica a partir de la mezcla del Etil-vínil Acetato (EVA) con neumáticos fuera de uso. Como exigencia principal se ha partido de unos costes mínimos de reciclado, es decir, mediante el uso del GTR sin desvulcanizary sin ningún pretratamiento previo con ácidos, pero en cambio actuando sobre la concentración y tamaños de las partículas del GTR, la cual se ha conseguido de una forma sencilla y económica como es el tamizado. Con este fin se han realizado análisis dieléctricos, mecánicos y térmicos de un gran número de compuestos obtenidos a partir de los tres tamaños de partículas del GTR (p<200μm, 200<p<500μm y p>500μm), y las siete concentraciones con las que se han formado los compuestos (0%, 5%, 10%, 20%, 40%, 50% y 70% en GTR). Todos estos datos han permitido una caracterización precisa de las propiedades de los nuevos compuestos y, de acuerdo con estos resultados y las Normativas vigentes, se han determinadoposibles aplicaciones eléctricas de los compuestos.

Structural and physico-mechanical properties of natural rubber/GTR composites devulcanized by microwaves: influence of GTR source and irradiation time

Ground tire rubber from car and truck was modified using microwave irradiation at variable time. The irradiated ground tire rubber was used as filler in composites based on natural rubber. The composites, with high content of ground tire rubber, were prepared using an internal batch mixer and subsequently cross-linked at 160¿. The influence of the ground tire rubber source (car/truck) and irradiation time on structure, physico-mechanical behaviour, thermal properties and morphology of natural rubber/ground tire rubber composites was studied. The interfacial interactions between ground tire rubber and natural rubber as function of ground tire rubber source and irradiation time were evaluated by Fourier transform infrared spectroscopy, thermogravimetric analysis, tensile tests, swelling measurements and scanning electron microscopy. The results showed that irradiation of ground tire rubber slightly enhanced tensile properties and cross-link density of natural rubber/ground tire rubber composites. This effect was more evident in the case of ground tire rubbertruck because of its higher content of natural rubber and was reflected in changes in the interfacial adhesion, which were confirmed by the results of Fourier transform infrared spectroscopy, thermogravimetric analysis and scanning electron microscopy analysisPostprint (author's final draft

Formation of iron-carbonate scale-layer and corrosion mechanism of API X70 pipeline steel in carbon dioxide-saturated 3% sodium chloride

This work presents the electrochemical results obtained during the study of the corrosion of X70 pipeline steel samples immersed in a 3 % wt NaCl solution saturated with CO2 at 20ºC under static and controlled turbulent flow conditions. In order to control the hydrodynamic conditions of the system, a rotating cylinder electrode (RCE) was used. Electrochemical techniques, Linear Polarization Resistance (LPR) and polarization curves, were used to determine the effect of turbulent flow upon the corrosion kinetics of the steel. We also elucidate the effect of temperature on the corrosion rate of the API X70 steel under the experimental conditions studied. Often, the corrosion of carbon steel in aqueous environments containing CO2 involves the formation of solid iron carbonate, FeCO3, as a corrosion product. The protective property of the formed FeCO3 scale layer to corrosion in brine solutions containing CO2 was established as the possible cause of the corrosion rate decrease above 60ºC

Influence of design parameters in the optimization of linear switched reluctance motor under thermal constraints

The objective of this paper is to present an original study for optimizing the size of the LongitudinalFlux Double-Sided Linear Switched Reluctance Motor (LSRM) under thermal and weight constraints. The performance is evaluated taken into account duty cycle operating conditions and thermal restrictions. The proposed approach couples Finite Element Analysis for magnetic propulsion force computation and Lumped Parameter Thermal Network for thermal transient analysis. The LSRMs design parameters are characterized by the number of phases and by their size denoted by the pole stroke. The operating conditions are the current density, the duty cycle and the admissible temperature rise of the insulation system. The grid search algorithm is used for solving the optimization problem. From the results, with the help of a novel multivariable optimization chart, a set of optimal configurations regarding to miniaturizations and downsizing of LSRMs is provided.Peer ReviewedPreprin

Aplicaciones eléctricas del acrilonitrilo butadieno estireno (ABS) reforzado con neumáticos fuera de uso (GTR)

La masiva fabricación de neumáticos, así como su posterior almacenamiento una vez utilizados, constituye un grave problema medioambiental al que se intenta dar salida de diversas formas, entre las que se encuentra el mezclar estos neumáticos fuera de uso (old used tires, GTR) con diferentes polímeros termoplásticos y termoestables. Estas mezclas se realizan variando el tratamiento previo al que se somete el GTR, el grado de desvulcanizado, las condiciones de mezcla o prensado, etc. Posteriormente, se analizan estas mezclas estructural y mecanicamente, buscando posibles aplicaciones industriales para ellas. El presente trabajo, pretende conseguir materiales aptos para la industria eléctrica a partir de la mezcal del Acrilonitrilo butadieno estireno (ABS) reutilizado con neumáticos fuera de uso (GTR), pero partiendo del requisito de unos mínimos costes de reciclado posibles, es decir, utilizando GTR vulcanizado y sin aplicar ningún pretratamiento previo, y en cambio, actuando sobre el tamaño de sus partículas, el cual se puede conseguir con un simple y economico tamizado. Otra novedad que introduce el presente estudio, es el elevado número de compuestos analizados, aparte del profundo analisis a los que se les ha sometido (dieléctrico, mecánico, térmico y de microestructura), obteniendose de cada ensayo una gran cantidad de variables. Los compuestos se obtuvieron tomando como variables los tres tamaños de particula del GTR (p<200μm, 200<p<500μm, y p>500μm), y las siete concentraciones de GTR en la mezcla (0%, 5%, 10%, 20%, 40%, 50% y70%), lo que representa, un total de 21 nuevos compuestos. Aparte, para que los ensayos dieléctricos fueran lo más exhaustivos posibles y mostraran el comportamiento del compuesto en condiciones muy diversas, se consideró un amplio régimen de temperaturas (30ºC hasta 120ºC) así como de frecuencias (1・10  -2 Hz hasta 3・106 Hz). Todos estos datos, han permitido caracterizar con bastante exactitud las propiedades de los nuevos compuestos, y dependiendo de estos resultados, se han buscado posibles aplicaciones eléctricas, con el requisito de que estas debían ajustarse a las Normativas Oficiales

Recreation noise in acoustic mapping

As far as most of citizens complains are about noise coming from recreational activities, usually related with the presence of people in the street around bars, pubs, parties and other activities carried out in public spaces, it seems reasonable to include this kind of noise source in the strategic noise maps, especially if the main objective of the noise mapping is to draw action plans. A methodology is proposed to analyze that kind of noise environments including short-term and long-term measurements, obtaining the acoustic indicators Ln and Lden as well as number of people exposed to those indicators. The results obtained in six different cities show that similar trends can be found for commercial streets in different cities, showing an increase of 5 dBA in Leq between 5 p.m and 8 p.m compared to the average value of Leq during the rest of day time. Night recreational noise seems more variable and an in field study must be carried out. It has also been found that around 10% of population is exposed to noise coming from commercial streets.Peer ReviewedPostprint (published version

Two-phase linear hybrid reluctance actuator with low detent force

In this paper, a novel two-phase linear hybrid reluctance actuator with the double-sided segmented stator, made of laminated U cores, and an interior mover with permanent magnets is proposed. The permanent magnets are disposed of in a way that increases the thrust force of a double-sided linear switched reluctance actuator of the same size. To achieve this objective, each phase of the actuator is powered by a single H-bridge inverter. To reduce the detent force, the upper and the lower stator were shifted. Finite element analysis was used to demonstrate that the proposed actuator has a high force density with low detent force. In addition, a comparative study between the proposed linear hybrid reluctance actuator, linear switched reluctance, and linear permanent magnet actuators of the same size was performed. Finally, experimental tests carried out in a prototype confirmed the goals of the proposed actuator.Peer ReviewedPostprint (published version

Waste aluminum application as energy valorization for hydrogen fuel cells for mobile low power machines applications

This article proposes a new model of power supply for mobile low power machines applications, between 10 W and 30 W, such as radio-controlled (RC) electric cars. This power supply is based on general hydrogen from residual aluminum and water with NaOH, so it is proposed energy valorization of aluminum waste. In the present research, a theoretical model allows us to predict the requested aluminum surface and the required flow of hydrogen has been developed, also considering, in addition to the geometry and purity of the material, two key variables as the temperature and the molarity of the alkaline solution used in the hydrogen production process. Focusing on hydrogen production, isopropyl alcohol plays a key role in the reactor’s fuel cell vehicle as it filters out NaOH particles and maintains a constant flow of hydrogen for the operation of the machine, keeping the reactor temperature controlled. Finally, a comparison of the theoretical and experimental data has been used to validate the developed model using aluminum sheets from ring cans to generate hydrogen, which will be used as a source of hydrogen in a power fuel cell of an RC car. Finally, the manuscript shows the parts of the vehicle’s powertrain, its behavior, and mode of operationPostprint (published version