Airway leukocyte number following LESB65 infection.

<p>Average B lymphocyte, CD4+ve T cell, CD8+ve T cell and neutrophil count shown as mean and standard deviation per mg of tissue at 24, 48, and 72 hours post infection [N = 5 each time point]. * = p<0.05, ** = p<0.01.</p

BAFF expression in BAL fluid.

<p>Expression was measured by ELISA in BAL fluid from <i>P. aeruginosa</i> positive [n = 6] and negative [n = 14] CF patients and healthy controls [n = 7]. BAFF expression was significantly elevated [p<0.01] in both the PA infected group and non PA infected group in comparison to the control group.</p

Expression of BAFF, CXCL13, CCL19 and CCL21 in mouse lung tissue.

<p>Each cytokine was measured at days 1, 2, 3 and 7 days after infection with 10⁶ CFU of <i>P. aeruginosa</i> LESB65 by ELISA. [n = 5]. BAFF was significantly expressed at days 1 [p<0.001], 2 [p<0.01], 3 [p<0.001], and 7 [p<0.05] in comparison to day 0 control. CXCL13 at days 1, 2, 3, [all p<0.001] and 7 [p<0.01]. CCL19 at days 1 [p<0.01], 2 [p<0.001], and 7 [p<0.001]. CCL21 at days 2 [p<0.001], 3 [p<0.01] and 7 [p<0.001]. * = p<0.05, ** = p<0.01, *** = p<0.001.</p

Kinetics of airway infection by <i>P. aeruginosa</i> LESB65.

<p>Mice were challenged [intranasal] with 10⁶ CFU. Values shown as an average Log CFU/mg of lung tissue homogenised, n = 5 mice at each time point. Time zero samples were taken at 15 minutes post infection to confirm entry into the lung and determine levels immediately post infection.</p

FACS analysis of lung inflammatory cells following <i>P. aeruginosa</i> LESB65 infection of mice.

<p>Representative FACS plots showing immune cell staining of airway cells. Each column shows plots from an individual mouse at each time point. All plots are lung CD45+ cells and gates were drawn relative to isotype control antibody staining for each marker. Top row shows B cells [CD45+, CD19+], middle row shows CD4 T cells [CD45+, CD3+, CD4+] and CD8 T cells [CD45+, CD3+, CD8+], bottom row shows neutrophils [CD4+, Gr-1hi, F4/80-] and monocytes/macrophages [CD45+, F4/80+, Gr-1int/low]. Numbers on gates are percentages of total CD45+ cells.</p